(data stored in ACNUC7421 zone)

HOGENOM: THIS3_1_PE1010

ID   THIS3_1_PE1010                       STANDARD;      PRT;   368 AA.
AC   THIS3_1_PE1010; D6CL24;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=tRNA-specific 2-thiouridylase mnmA; EC=2.8.1 -;
DE   (THIS3_1.PE1010).
GN   Name=trmU; Synonyms=mnmA; OrderedLocusNames=THI_1081;
OS   THIOMONAS SP. 3AS.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Thiomonas.
OX   NCBI_TaxID=426114;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS THIS3_1.PE1010.
CC       Thiomonas sp. (strain 3As) chromosome, complete sequence.
CC       genome.
CC   -!- ANNOTATIONS ORIGIN:D6CL24_THIS3
CC   -!- FUNCTION: Catalyzes the 2-thiolation of uridine at the wobble
CC       position (U34) of tRNA, leading to the formation of s(2)U34 (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the mnmA/TRMU family.
CC   -!- GENE_FAMILY: HOG000218046 [ FAMILY / ALN / TREE ]
DR   UniProtKB/Swiss-Prot; D6CL24; -.
DR   EMBL; FP475956; CAZ87777.1; -; Genomic_DNA.
DR   ProteinModelPortal; D6CL24; -.
DR   GenomeReviews; FP475956_GR; THI_1081.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_00144; tRNA_thiouridyl_MnmA; 1; -.
DR   InterPro; IPR023382; Adenine_a_hdrlase_dom.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR004506; tRNA-specific_2-thiouridylase.
DR   Gene3D; G3DSA:2.30.30.280; G3DSA:2.30.30.280; 1.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR11933; TrmU_mtfrase; 1.
DR   Pfam; PF03054; tRNA_Me_trans; 1.
DR   TIGRFAMs; TIGR00420; TrmU; 1.
DR   HOGENOMDNA; THIS3_1.PE1010; -.
KW   CAZ87777.123.19671820036002503210000011;
KW   tRNA-specific 2-thiouridylase mnmA ;
KW   ATP-binding; Complete proteome; Cytoplasm; Disulfide bond;
KW   Methyltransferase; Nucleotide-binding; RNA-binding; Transferase;
KW   tRNA processing; tRNA-binding.
SQ   SEQUENCE   368 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MTSSSRGIVV VGLSGGVDSS VSAWLLKQQG YTVVGLFMKN WEDDDDSEYC STRQDWLDAA
     SVADRIGIDI EAVNFAAEYK ERVFAEFLRE YAAGRTPNPD VLCNAEIKFK AFLDHAMRLG
     ADRIATGHYA RVRETDHGFE LLRGLDASKD QSYFLHRLNQ AQLARTLFPV GELNKTEVRK
     VAETIGLHNA RKKDSTGICF IGERPFRDFL SRYLPTQPGA MKTPEGKTVG EHLGLAFYTL
     GQRKGIGLGG SNSGNGEPWF VAGKDLERNQ LIVAQGHDHP LLLSHTLQAL SASWVSGTAP
     TEEVFGYGAK TRYRQVDAAC RFQDQGPERF TLKFDAPQWA VTPGQSAVLY DGEVCLGGGI
     IEAASRND
//

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