(data stored in ACNUC14238 zone)

HOGENOM: YEAST2-MICRON_PE1

ID   YEAST2-MICRON_PE1                    STANDARD;      PRT;   423 AA.
AC   YEAST2-MICRON_PE1; P03870;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Site-specific recombinase Flp; Short=FLP;AltName:
DE   Full=Protein Able; (YEAST2-MICRON.PE1).
GN   Name=FLP1; OrderedLocusNames=R0010W;
OS   SACCHAROMYCES CEREVISIAE.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomyceta; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS YEAST2-MICRON.PE1.
CC       Saccharomyces cerevisiae chromosome 2-micron EF2 full sequence 1..6318
CC       annotated by Ensembl
CC   -!- ANNOTATIONS ORIGIN:FLP_YEAST
CC   -!- FUNCTION: Part of the plasmid amplification system, which corrects
CC       any decrease in copy number caused by a rare missegregation event.
CC       Catalyzes the recombination between the large inverted repetitions
CC       of the 2-micron plasmid during plasmid replication. This
CC       recombination event changes the direction of one of the two
CC       replication forks in the bidirectionally replicating molecule,
CC       effectively resulting in multiple rounds of replication from a
CC       single initiation event. Binds specifically to the FLP recognition
CC       target (FRT) site where it induces DNA to bend. Three types of
CC       bend exist. Type I is approximately 60 degrees and results from 1
CC       FLP molecule binding to 1 symmetry element. Type II is >144
CC       degrees and results from FLP molecules binding to symmetry
CC       elements a and b. Type III is approximately 65 degrees and results
CC       from FLP molecules binding to symmetry elements b and c.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- INDUCTION: Repressed by the negative regulatory complex REP1-REP2.
CC   -!- MISCELLANEOUS: The plasmid 2-micron circle is a extrachromosomal
CC       element that resides in the nucleus and propagates itself stably
CC       in host cell populations. It provides no obvious advantage to the
CC       host but imposes no significant disadvantage either at its steady-
CC       state copy number of 40-60 molecules/cell.
CC   -!- SIMILARITY: Belongs to the 'phage' integrase family.
CC   -!- GENE_FAMILY: HBG000000000 [ FAMILY / ALN / TREE ]
DR   HOGENOM:Saccharomyces_cerevisiae;R0010W;R0010W;R0010W.
DR   EMBL; J01347; - ;
DR   EMBL; Y10429; - ;
DR   UniProtKB/Swiss-Prot; P03870; -.
DR   EMBL; J01347; AAB59340.1; -; Genomic_DNA.
DR   PIR; A04502; PDBYA.
DR   RefSeq; NP_040488.1; NC_001398.1.
DR   PDB; 1FLO; X-ray; 2.65 A; A/B/C/D=2-423.
DR   PDB; 1M6X; X-ray; 2.80 A; A/B/C/D=3-423.
DR   PDB; 1P4E; X-ray; 2.70 A; A/B=3-423, C/D=3-422.
DR   PDBsum; 1FLO; -.
DR   PDBsum; 1M6X; -.
DR   PDBsum; 1P4E; -.
DR   ProteinModelPortal; P03870; -.
DR   SMR; P03870; 2-422.
DR   STRING; P03870; -.
DR   EnsemblFungi; R0010W; R0010W; R0010W.
DR   CYGD; R0010w; -.
DR   SGD; S000029654; FLP1.
DR   ArrayExpress; P03870; -.
DR   Genevestigator; P03870; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013762; Integrase-like_cat-core.
DR   InterPro; IPR010998; Integrase_Lambda-type_N.
DR   InterPro; IPR005626; Recombinase_Flp.
DR   InterPro; IPR022647; Recombinase_Flp_N.
DR   Gene3D; G3DSA:1.10.443.10; Phage_intgr_like; 1.
DR   Pfam; PF05202; Flp_C; 1.
DR   Pfam; PF03930; Flp_N; 1.
DR   SUPFAM; SSF56349; DNA_brk_join_enz; 1.
DR   SUPFAM; SSF47823; L_intgrse_like_N; 1.
DR   HOGENOMDNA; YEAST2-MICRON.PE1; -.
KW   R0010W37.100026476820036002503210000011; R0010W41.1; FLP_YEAST;
KW   J01347; Y10429;
KW   3D-structure; Complete proteome; Direct protein sequencing;
KW   DNA integration; DNA recombination; DNA-binding; Plasmid;
KW   Reference proteome.
SQ   SEQUENCE   423 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MPQFGILCKT PPKVLVRQFV ERFERPSGEK IALCAAELTY LCWMITHNGT AIKRATFMSY
     NTIISNSLSF DIVNKSLQFK YKTQKATILE ASLKKLIPAW EFTIIPYYGQ KHQSDITDIV
     SSLQLQFESS EEADKGNSHS KKMLKALLSE GESIWEITEK ILNSFEYTSR FTKTKTLYQF
     LFLATFINCG RFSDIKNVDP KSFKLVQNKY LGVIIQCLVT ETKTSVSRHI YFFSARGRID
     PLVYLDEFLR NSEPVLKRVN RTGNSSSNKQ EYQLLKDNLV RSYNKALKKN APYSIFAIKN
     GPKSHIGRHL MTSFLSMKGL TELTNVVGNW SDKRASAVAR TTYTHQITAI PDHYFALVSR
     YYAYDPISKE MIALKDETNP IEEWQHIEQL KGSAEGSIRY PAWNGIISQE VLDYLSSYIN
     RRI
//

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