(data stored in ACNUC14238 zone)

HOGENOM: YEAST2-MICRON_PE4

ID   YEAST2-MICRON_PE4                    STANDARD;      PRT;   296 AA.
AC   YEAST2-MICRON_PE4; P03872;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Partitioning protein REP2; Short=R2;AltName: Full=Protein
DE   Charlie;AltName: Full=Trans-acting factor C; (YEAST2-MICRON.PE4).
GN   Name=REP2; OrderedLocusNames=R0040C;
OS   SACCHAROMYCES CEREVISIAE.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomyceta; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS YEAST2-MICRON.PE4.
CC       Saccharomyces cerevisiae chromosome 2-micron EF2 full sequence 1..6318
CC       annotated by Ensembl
CC   -!- ANNOTATIONS ORIGIN:REP2_YEAST
CC   -!- FUNCTION: Part of the plasmid partitioning system, which ensures
CC       the equal distribution of replicated plasmid molecules to daugther
CC       cells. The plasmids exist as well-organized plasmid foci within
CC       the nucleus that stay together throughout the cell-cycle and act
CC       as entity during segregation, effetively reducing copy number to
CC       one. Plasmid partitioning requires the proteins REP1, REP2, and a
CC       cis-acting locus STB (REP3). REP1-REP2 stably associate with CSE4-
CC       containing chromatin at STB during S-phase, marking the locus with
CC       a centromeric tag, and thereby probably catching mitotic spindle
CC       microtubules to the plasmid cluster and coupling plasmid
CC       segregation to chromosome segregation. REP1-REP2 are required to
CC       recruit the cohesin complex to the STB locus for pairing of the
CC       replicated plasmid cluster, a prerequisite for successful plasmid
CC       segregation. REP1-REP2 also negatively regulate expression of
CC       site-specific recombinase FLP and of RAF1.
CC   -!- SUBUNIT: Interacts with REP1.
CC   -!- INTERACTION:
CC       Self; NbExp=5; IntAct=EBI-2125362, EBI-2125362;
CC       P03871:REP1; NbExp=7; IntAct=EBI-2125362, EBI-14929;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with the STB locus
CC       of the 2-micron plasmid as foci in the nucleus near the spindle
CC       pole body. Is expelled from STB during a short interval between
CC       late G1 and early S phases.
CC   -!- MISCELLANEOUS: The plasmid 2-micron circle is a extrachromosomal
CC       element that resides in the nucleus and propagates itself stably
CC       in host cell populations. It provides no obvious advantage to the
CC       host but imposes no significant disadvantage either at its steady-
CC       state copy number of 40-60 molecules/cell.
CC   -!- GENE_FAMILY: HBG000000000 [ FAMILY / ALN / TREE ]
DR   HOGENOM:Saccharomyces_cerevisiae;R0040C;R0040C;R0040C.
DR   EMBL; J01347; - ;
DR   UniProtKB/Swiss-Prot; P03872; -.
DR   EMBL; J01347; AAB59343.1; -; Genomic_DNA.
DR   PIR; A04504; PDBYC.
DR   RefSeq; NP_040491.1; NC_001398.1.
DR   ProteinModelPortal; P03872; -.
DR   IntAct; P03872; 3.
DR   STRING; P03872; -.
DR   EnsemblFungi; R0040C; R0040C; R0040C.
DR   CYGD; R0040c; -.
DR   SGD; S000029676; REP2.
DR   ArrayExpress; P03872; -.
DR   Genevestigator; P03872; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0030541; P:plasmid partitioning; IEA:UniProtKB-KW.
DR   HOGENOMDNA; YEAST2-MICRON.PE4; -.
KW   R0040C37.100026476820036002503210000011; R0040C41.1; REP2_YEAST; J01347;
KW   Complete proteome; Nucleus; Phosphoprotein; Plasmid;
KW   Plasmid partition; Reference proteome.
SQ   SEQUENCE   296 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MDDIETAKNL TVKARTAYSV WDVCRLFIEM IAPDVDIDIE SKRKSDELLF PGYVIRPMES
     LTTGRPYGLD SSAEDSSVSS DSSAEVILPA AKMVKERFDS IGNGMLSSQE ASQAAIDLML
     QNNKLLDNRK QLYKSIAIII GRLPEKDKKR ATEMLMRKMD CTQLLVPPAP TEEDVMKLVS
     VVTQLLTLVP PDRQAALIGD LFIPESLKDI FNSFNELAAE NRLQQKKSEL EGRTEVNHAN
     TNEEVPSRRT RSRDTNARGA YKLQNTITEG PKAVPTKKRR VATRVRGRKS RNTSRV
//

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