(data stored in ACNUC9543 zone)

HOGENOM: YEASTXI_PE327

ID   YEASTXI_PE327                        STANDARD;      PRT;   1071 AA.
AC   YEASTXI_PE327; P15938; D6VXE6;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16;
DE   EC=3.6.4 13; (YEASTXI.PE327).
GN   Name=PRP16; OrderedLocusNames=YKR086W; ORFNames=YKR406;
OS   SACCHAROMYCES CEREVISIAE.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomyceta; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS YEASTXI.PE327.
CC       Saccharomyces cerevisiae chromosome XI EF2 full sequence 1..666454
CC       annotated by Ensembl
CC   -!- ANNOTATIONS ORIGIN:PRP16_YEAST
CC   -!- FUNCTION: Influences the fidelity of branchpoint recognition in
CC       yeast splicing. This is RNA-dependent ATPase which is essential
CC       for viability. It may mediate one of the many ATP-requiring steps
CC       of spliceosome assembly and that accuracy of branchpoint
CC       recognition may be coupled to ATP binding and/or hydrolysis.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Note=Binds to the spliceosome.
CC   -!- MISCELLANEOUS: Present with 1250 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH
CC       subfamily. PRP16 sub-subfamily.
CC   -!- SIMILARITY: Contains 1 helicase ATP-binding domain.
CC   -!- SIMILARITY: Contains 1 helicase C-terminal domain.
CC   -!- GENE_FAMILY: HOG000175261 [ FAMILY / ALN / TREE ]
DR   HOGENOM:Saccharomyces_cerevisiae;YKR086W;YKR086W;YKR086W.
DR   EMBL; M31524; - ;
DR   EMBL; Z27116; - ;
DR   EMBL; Z28311; - ;
DR   UniProtKB/Swiss-Prot; P15938; D6VXE6; -.
DR   EMBL; M31524; AAA34911.1; -; Genomic_DNA.
DR   EMBL; Z27116; CAA81637.1; -; Genomic_DNA.
DR   EMBL; Z28311; CAA82165.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09236.1; -; Genomic_DNA.
DR   PIR; S38164; S38164.
DR   RefSeq; NP_013012.1; NM_001179876.1.
DR   ProteinModelPortal; P15938; -.
DR   SMR; P15938; 335-988.
DR   DIP; DIP-2824N; -.
DR   IntAct; P15938; 20.
DR   MINT; MINT-1699731; -.
DR   STRING; P15938; -.
DR   PeptideAtlas; P15938; -.
DR   EnsemblFungi; YKR086W; YKR086W; YKR086W.
DR   GeneID; 853961; -.
DR   KEGG; sce:YKR086W; -.
DR   NMPDR; fig|4932.3.peg.3999; -.
DR   CYGD; YKR086w; -.
DR   SGD; S000001794; PRP16.
DR   eggNOG; fuNOG04053; -.
DR   GeneTree; EFGT00050000000163; -.
DR   OMA; IDCGYSK; -.
DR   OrthoDB; EOG4GQTD2; -.
DR   PhylomeDB; P15938; -.
DR   NextBio; 975386; -.
DR   ArrayExpress; P15938; -.
DR   Genevestigator; P15938; -.
DR   GermOnline; YKR086W; Saccharomyces cerevisiae.
DR   GO; GO:0071007; C:U2-type catalytic step 2 spliceosome; IDA:SGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0008186; F:RNA-dependent ATPase activity; IDA:SGD.
DR   GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IMP:SGD.
DR   InterPro; IPR014001; DEAD-like_helicase.
DR   InterPro; IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR011709; DUF1605.
DR   InterPro; IPR007502; Helicase-assoc_dom.
DR   InterPro; IPR001650; Helicase_C.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF04408; HA2; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF07717; OB_NTP_bind; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00847; HA2; 1.
DR   SMART; SM00490; HELICc; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   HOGENOMDNA; YEASTXI.PE327; -.
KW   YKR086W-A100026476820036002503210000011; YKR086W-A1; PRP16_YEAST; M31524;
KW   Z28311;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing;
KW   mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome.
SQ   SEQUENCE   1071 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MGHSGREERI KDIFKELTSK ELTPGLLLTL QKLAQKPNTN LEQFIASCKA LTKLSSNNPI
     IFNELLELLK NKSEEDSTGP KKIAPSINKR KKFKIQLDLD DNEDELDSPV QKKPAPTRTL
     FKRIDKLKAK QLRQYSPTVK DPSPNSEQQT QNGHAETKDY EPTRSEVVEE DREWYDNDDD
     YGNLVPEPLS ELPEEAKLLP VIRNIDNDDA LRNTVQLYPI PLKQRMEWIP PFLSKFALEN
     KVPTSIIIGS ISETSSQVSA LSMVNPFRNP DSEFSANAKR GSKLVALRRI NMEHIQQSRD
     NTTVLNTAMG EVLGLENNNK AKDKSNQKIC DDTALFTPSK DDIKHTKEQL PVFRCRSQLL
     SLIRENQVVV IIGETGSGKT TQLAQYLYEE GYANDRGKSI VVTQPRRVAA ISVAKRVAME
     MQVPLGKEVG YSIRFEDVTD SECTKLKFVT DGILLRETLL DDTLDKYSCV IIDEAHERSL
     NTDILLGFFK ILLARRRDLK LIITSATMNA KKFSAFFGNA PQFTIPGRTF PVQTIYTSNP
     VQDYVEAAVS QAVKIHLAND CSSGDILIFM TGQEDIETTF DTLQEKFLQV YSKKFGTANF
     EEINDIEILP IYSALPADLQ FKIFQDLHGT KRKIIIATNI AETSLTIKGI RYVIDCGYSK
     LKVYNPKIGL DSLVITPISK ANADQRSGRA GRTAPGTAYR LYTEDTFKED MYLQTIPEIQ
     RTNLSNTLLL LKSLDVTDEL SKFPFIDKPP LQTFLSSLYE LWFIGAIDTS GQLTPLGLQM
     AKFPLQPSLS KILLIAVRNG CSDEMLTIVS MLSVPQVFYR PKERQKEADI ARNKFFIAKS
     DHLTLLNVFE QWRANNFSSH WCNKHFVQYK SLVRARDIRD QLLTILKSQK IPVISSGKDW
     DIIKKCICSG FAHQAAKITG LRNYVHLKTG VSVQLHPTSA LHGLGDLPPY VVYHELLMTS
     KEYICCVTSV DPFWLMEYGG LLYDIKRIKN DQEATTTGLF GEHYEHTLDK VEDDIDINIR
     RCKDMRDSVI QELKMTDNSN KEDKKQKTKK QNILNGKENS MKPFKRRKPF F
//

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