(data stored in ACNUC8465 zone)

HOGENOM: YEASTX_PE104

ID   YEASTX_PE104                         STANDARD;      PRT;   668 AA.
AC   YEASTX_PE104; P08018; D6VW57;
DT   00-JAN-0000 (Rel. 1, Created)
DT   00-JAN-0000 (Rel. 2, Last sequence update)
DT   00-JAN-0000 (Rel. 3, Last annotation update)
DE   RecName: Full=MAP kinase kinase PBS2; EC=2.7.12 2;AltName: Full=Polymyxin
DE   B resistance protein 2;AltName: Full=Suppressor of fluoride sensitivity
DE   4; (YEASTX.PE104).
GN   Name=PBS2; Synonyms=HOG4, SFS4, SSK4; OrderedLocusNames=YJL128C;
OS   SACCHAROMYCES CEREVISIAE.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomyceta; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [0]
RP   -.;
RG   -.;
RL   -.;
CC   -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS YEASTX.PE104.
CC       Saccharomyces cerevisiae chromosome X EF2 full sequence 1..745741 annot
CC       by Ensembl
CC   -!- ANNOTATIONS ORIGIN:PBS2_YEAST
CC   -!- FUNCTION: Kinase involved in a signal transduction pathway that is
CC       activated by changes in the osmolarity of the extracellular
CC       environment. Seems to phosphorylate HOG1 on a tyrosine residue.
CC   -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
CC   -!- SUBUNIT: Interacts with NBP2, PTC1, SHO1 AND STE11.
CC   -!- INTERACTION:
CC       P40073:SSU81; NbExp=2; IntAct=EBI-12972, EBI-18140;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DOMAIN: Alternative way of activation involves binding the
CC       proline-rich motif to the SH3 domain of SHO1.
CC   -!- PTM: Activated by phosphorylation by SSK2 or SSK22. Ser/Thr
CC       phosphorylation is also necessary for SHO1-mediated activation.
CC   -!- MISCELLANEOUS: Present with 2160 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr
CC       protein kinase family. MAP kinase kinase subfamily.
CC   -!- SIMILARITY: Contains 1 protein kinase domain.
CC   -!- GENE_FAMILY: HOG000234206 [ FAMILY / ALN / TREE ]
DR   HOGENOM:Saccharomyces_cerevisiae;YJL128C;YJL128C;YJL128C.
DR   EMBL; J02946; - ;
DR   EMBL; U12237; - ;
DR   EMBL; Z49403; - ;
DR   UniProtKB/Swiss-Prot; P08018; D6VW57; -.
DR   EMBL; J02946; AAA16819.1; -; Unassigned_DNA.
DR   EMBL; U12237; AAA20392.1; -; Genomic_DNA.
DR   EMBL; Z49403; CAA89423.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08673.2; -; Genomic_DNA.
DR   PIR; S56909; S56909.
DR   RefSeq; NP_012407.2; NM_001181561.2.
DR   PDB; 2VKN; X-ray; 2.05 A; C=92-103.
DR   PDBsum; 2VKN; -.
DR   ProteinModelPortal; P08018; -.
DR   SMR; P08018; 355-639.
DR   DIP; DIP-2368N; -.
DR   IntAct; P08018; 19.
DR   MINT; MINT-546167; -.
DR   STRING; P08018; -.
DR   PeptideAtlas; P08018; -.
DR   EnsemblFungi; YJL128C; YJL128C; YJL128C.
DR   GeneID; 853313; -.
DR   KEGG; sce:YJL128C; -.
DR   NMPDR; fig|4932.3.peg.3374; -.
DR   CYGD; YJL128c; -.
DR   SGD; S000003664; PBS2.
DR   eggNOG; fuNOG05342; -.
DR   GeneTree; EFGT00050000000597; -.
DR   OMA; LQKIPER; -.
DR   OrthoDB; EOG4RV60P; -.
DR   PhylomeDB; P08018; -.
DR   BRENDA; 2.7.12.2; 984.
DR   NextBio; 973654; -.
DR   ArrayExpress; P08018; -.
DR   Genevestigator; P08018; -.
DR   GermOnline; YJL128C; Saccharomyces cerevisiae.
DR   GO; GO:0005935; C:cellular bud neck; IDA:SGD.
DR   GO; GO:0005934; C:cellular bud tip; IDA:SGD.
DR   GO; GO:0031416; C:NatB complex; IDA:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004708; F:MAP kinase kinase activity; IMP:SGD.
DR   GO; GO:0005078; F:MAP-kinase scaffold activity; IPI:SGD.
DR   GO; GO:0004596; F:peptide alpha-N-acetyltransferase activity; IMP:UniProtKB.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0007015; P:actin filament organization; IMP:SGD.
DR   GO; GO:0000169; P:activation of MAPK activity involved in osmosensory signaling pathway; IMP:SGD.
DR   GO; GO:0034605; P:cellular response to heat; IMP:SGD.
DR   GO; GO:0006972; P:hyperosmotic response; IMP:SGD.
DR   GO; GO:0000208; P:MAPK import into nucleus involved in osmosensory signaling pathway; IMP:SGD.
DR   GO; GO:0017196; P:N-terminal peptidyl-methionine acetylation; IGI:UniProtKB.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR011009; Kinase-like_dom.
DR   InterPro; IPR000719; Prot_kinase_cat_dom.
DR   InterPro; IPR017442; Se/Thr_kinase-like_dom.
DR   InterPro; IPR008271; Ser/Thr_kinase_AS.
DR   InterPro; IPR002290; Ser/Thr_kinase_dom.
DR   Pfam; PF00069; Pkinase; 1.
DR   SMART; SM00220; S_TKc; 1.
DR   SUPFAM; SSF56112; Kinase_like; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; FALSE_NEG.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR   HOGENOMDNA; YEASTX.PE104; -.
KW   YJL128C-A100026476820036002503210000011; YJL128C-A1; PBS2_YEAST; J02946;
KW   Z49403;
KW   3D-structure; Antibiotic resistance; ATP-binding; Complete proteome;
KW   Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Serine/threonine-protein kinase; Transferase;
KW   Tyrosine-protein kinase.
SQ   SEQUENCE   668 AA;  UNKNOWN MW;  UNKNOWN CRC64;
     MEDKFANLSL HEKTGKSSIQ LNEQTGSDNG SAVKRTSSTS SHYNNINADL HARVKAFQEQ
     RALKRSASVG SNQSEQDKGS SQSPKHIQQI VNKPLPPLPV AGSSKVSQRM SSQVVQASSK
     STLKNVLDNQ ETQNITDVNI NIDTTKITAT TIGVNTGLPA TDITPSVSNT ASATHKAQLL
     NPNRRAPRRP LSTQHPTRPN VAPHKAPAII NTPKQSLSAR RAVKLPPGGM SLKMPTKTAQ
     QPQQFAPSPS NKKHIETLSN SKVVEGKRSN PGSLINGVQS TSTSSSTEGP HDTVGTTPRT
     GNSNNSSNSG SSGGGGLFAN FSKYVDIKSG SLNFAGKLSL SSKGIDFSNG SSSRITLDEL
     EFLDELGHGN YGNVSKVLHK PTNVIMATKE VRLELDEAKF RQILMELEVL HKCNSPYIVD
     FYGAFFIEGA VYMCMEYMDG GSLDKIYDES SEIGGIDEPQ LAFIANAVIH GLKELKEQHN
     IIHRDVKPTN ILCSANQGTV KLCDFGVSGN LVASLAKTNI GCQSYMAPER IKSLNPDRAT
     YTVQSDIWSL GLSILEMALG RYPYPPETYD NIFSQLSAIV DGPPPRLPSD KFSSDAQDFV
     SLCLQKIPER RPTYAALTEH PWLVKYRNQD VHMSEYITER LERRNKILRE RGENGLSKNV
     PALHMGGL
//

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