Alignment of the gene Family HOG000231362 of HOGENOM

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Isoforms are ON
AILMEGL192563_1_1_PE14 NOISO ADAE----------DGSLRPAGPSAAPISFSFSRTSARRRLADPGD------DAGAAPEE ANOCA553_PE5 NOISO -------------------------APVSFGFTRTTGRRRFLV----VTGQ-DGGEAKQE BOVINX_57_PE10 NOISO ADAE----------DGVLRPAGSSTAPISFSFNRTSARRWLAGD-----------AAPEE CALJAX_48_PE57 NOISO ADSE----------EVVLPLTAASTAPISFGFTRTSARRRLADSGD------GAGPSPEE CANFAX_43_PE41 NOISO ADAE----------EYPLRPAGSSAAPISFSFSRTTARRRLADPVD------DAGAAPEE CAVPO132_3_PE19 NOISO --AE----------EEVSEPATLSAAPVSFGFTRTSVRRRLADKAD------GTGSAPEE DANRE8_40_PE13 ENSDARG00000029612 ASPERYQERESLKKDISQDDGGKKAAPISFGFSKTLIKVK-------SG-------KAEE DANRE8_40_PE14 ISOFORMIN ASPERYQERESLKKDISQDDGGKKAAPISFGFSKTLIKVK-------SG-------KAEE DASNOGS_505_PE1 NOISO EDAE----------ERVSRPAAASTGPISFGFSRTSVRRRVADAGN------SGGTAPEE DIPORGS_488_PE1 NOISO EDHE----------EGILRPAEISTATVSFGFTRTSSRRQLADLGA------SAKTAPEE ERIEUGS_624_PE1 NOISO EEAE----------EGVSEPAVPSSAPISFTFSRTSARRRLAESGD------GAGAALEE FELCAGS_330_PE1 NOISO ------------------------------------------------------------ FUGRU226_PE32 ENSTRUG00000000616 ASNG--ESV-----LALEDQEERKPAAVSFGFTKTISKFK-------SSGA-DVSSNKDE FUGRU226_PE33 ISOFORMIN ASNG--ESV-----LALEDQEERKPAAVSFGFTKTISKFK-------SSGA-DVSSNKDE FUGRU226_PE34 ISOFORMIN ASNG--ESV-----LALEDQEERKPAAVSFGFTKTISKFK-------SSGA-DVSSNKDE GASACGROUPXII_17_PE10 ENSGACG00000012127 ASLG--EDTGSPKSAPPGDDEERKPMAVSFGFTKTVNKFK-------PSTG-E--FRKDD GASACGROUPXII_17_PE11 ISOFORMIN ASLG--EDTGSPKSAPPGDDEERKPMAVSFGFTKTVNKFK-------PSTG-E--FRKDD GORGOX_52_PE26 NOISO ADSE----------EGVLPLTAASTAPISFGFTRTSARRRLADSGD------GAGPSPEE HORSEX_41_PE12 NOISO ADAE----------EGVLRPAGASTAPISFGFSRTSARRRLADPGD------GAGAPPEE HSX_PE902 ENSG00000068394 ADSK----------EGVLPLTAASTAPISFGFTRTSARRRLADSGD------GAGPSPEE HSX_PE903 ISOFORMIN ADSK----------EGVLPLTLPLPQFHSASLARPHGGGRL---GR------RRGAISGG LOXAF56_2_PE8 NOISO ADAE----------EGVSRPAAVSTAPISFGFSRTSSRRRVVDLGD------RARAAPEE MACEUGS_741_PE1 NOISO ------------------------------------------------------------ MACMUX_48_PE33 NOISO ------------------------------------------------------------ MICMU15422_PE1 NOISO ADAE----------DRVLQLAATSTAPISFGFTRKSARRRLXXXXX------XXXXXXXX MONDO4_393_PE11 NOISO V-NGQ----D--GATATPAQVSGPPGLVSFGFSRTAPRKRLAEGREGPPGQ-RGEEEEEE MONDOUN_64_PE2 NOISO ------------------------------------------------------------ MOUSEX_PE58 ENSMUSG00000031148 -MAG----------RESPPPSAPSMAPISFGFTRTSVRRRLADLGD------SERQAPEE MOUSEX_PE59 ISOFORMIN ------------------------------------------------------------ NEMVE_9979_PE55 NOISO -------------------MADEASKPLSFGFTKKRESKQLINTGKGVLGNEHGIEKEEE OCHPRGS_331_PE1 NOISO ADAE----------DNVWPPAAPSTASVSFAFSRTSSRKRLVVLTD------GAESASEE ORNANULTRA295_PE13 NOISO A--------------DDDEGPPVAPAPVSFSFTRTAARKRVVEP----KGG---KDAVEE ORYLA7_22_PE39 NOISO AAQG--EGTAAFKSVPFEDKGQKKTSMVSFGFTKTVNKFK-------TPSA-EESKKKEE OTOGAGS_386_PE1 NOISO ------------------------------------------------------------ PIGX_44_PE8 NOISO ADAK----------DGVLRPAGSSTAPVSFSFSRTSNRKRLAGD-----------AAPEE PONPYX_50_PE28 NOISO ADSE----------EGVLPLTAASTAPISFGFTRTSARRRLADSGD------GAGPSPEE RABITX_35_PE16 NOISO ADAE----------DGVLQPTAPSTAPISFAFTRTSSRRRLVALSD------GAESAPEE RATX_PE254 NOISO -MAG----------RESPPPAAASTAPISFGFTRTSVRRRLADLGD------SEGQAPEK SORARGS_498_PE1 NOISO ------------------------------------------------------------ SPETRGS_404_PE1 NOISO ------------------------------------------------------------ TETNG9_PE628 ENSTNIG00000012502 ASDG--EDV-----LAFEDREERKPTAVSFGFTKTISKFK-------PQSA-DASSNKDE TETNG9_PE629 ISOFORMIN ASDG--EDV-----LAFEDREERKPTAVSFGFTKTISKFK-------PQSA-DASSNKDE TRIAD_1217_PE476 NOISO -------------------MDDKKTSTISFTLNKNFAPKKLNTSKY-----NEEEKIEDD TUPGBGS_385_PE1 NOISO ADAE----------DLVLQPAAASSAPISFGFTRTSARRRLADLGD------GVGPTPED TURTR89660_PE1 NOISO ADAK----------DGVLWPAGASSAPISFTFSRTSARRWLAG------------DAAPE XENTR154_2_PE15 NOISO -----------------MAAGGSSQAPVSFGFSRSSKK-KFLV----ANGD-E--REEDE MATCH CONSENSUS ????????????????????????????SF??????????????????????????????

AILMEGL192563_1_1_PE14 NOISO KDFLKTVEGRELKSVKP-SETPK---ELVIPLIQNGHRRQPPTQALGPST-----NTDA- ANOCA553_PE5 NOISO PDFLSAVEGRELQSVRP-APQPK---ELVIPLIQKNQWKKPAALLEPDA----------- BOVINX_57_PE10 NOISO KDFLKTVEGRELQSVKP-SEAPK---ELVIPLIQNGHRRQPPTQAPGPST-----HTEV- CALJAX_48_PE57 NOISO KDFLKSVEGRELQSVKP-QEAPT---ELIIPLIQSGYRRQPPAPSPGPSA-----DSGA- CANFAX_43_PE41 NOISO KDFLKTVEGRELQSVKP-SENPK---DLIIPLIQNGHRRQPPAQAPGPST-----NTEA- CAVPO132_3_PE19 NOISO KDFLKVVEGRELQSVKP-LEAPK---ELVIPLIQSGFRRQPLAQTPMPSV-------EA- DANRE8_40_PE13 ENSDARG00000029612 RDYLVEVEGKELKGTKP-VEKPK---ELIIPLIHKNRWYRQDAERADKTSEEKTEEPSEV DANRE8_40_PE14 ISOFORMIN RDYLVEVEGKELKGTKP-VEKPK---ELIIPLIHKNRWYRQDAERADKTSEEKTEEPSEV DASNOGS_505_PE1 NOISO KDFLKTVEGRELQSVKP-SEVPK---ELIIPLIQNGHRRQPPTQAPGPSS-----NTET- DIPORGS_488_PE1 NOISO KEFLNTIEGEFQXXXXX-XXXXX---XXXXXXXXXXXXXXXXXXXXXXXX-----XXXX- ERIEUGS_624_PE1 NOISO KDFLRTVEGRELHSVKP-TEAPK---ELVIPLIKNACRRQPATQAPGAST-----DTE-- FELCAGS_330_PE1 NOISO --------------VKP-SETPK---ELVIPLIQNGHRRQPPTQAPGPST-----NTEA- FUGRU226_PE32 ENSTRUG00000000616 KEYLTGVDKRLLQS--S-SEKPK---ELIIPLIVKNRWHKQGQSE------GDG-EKTAQ FUGRU226_PE33 ISOFORMIN KEYLTGVDKRLLQS--S-SEKPK---ELIIPLIVKNRWHKQGQSE------GDG-EKTAQ FUGRU226_PE34 ISOFORMIN KEYLTGVDKRLLQRYDS-SEKPK---ELIIPLIVKNRWHKQGQSE------GDG-EKTAQ GASACGROUPXII_17_PE10 ENSGACG00000012127 RDYLTGIDGKELQSTKP-SEKPK---ELIIPLIQKNRWHRPDQAVQAETRAVKT-PKNTE GASACGROUPXII_17_PE11 ISOFORMIN RDYLTGIDGKELQSTKP-SEKPK---ELIIPLIQKNRWHRPDQAVQAETRAVKT-PKNTE GORGOX_52_PE26 NOISO KDFLKTVEGRELQSVKP-QEAPK---ELVIPLIQNGHRRQPPAWPPGPST-----DTGA- HORSEX_41_PE12 NOISO KDFLKTVEGRELQSVKP-SEAPK---ELVIPLIQNGHRRQPLTQAPGPST-----DTEA- HSX_PE902 ENSG00000068394 KDFLKTVEGRELQSVKP-QEAPK---ELVIPLIQNGHRRQPPARPPGPST-----DTGA- HSX_PE903 ISOFORMIN EGFLENRGREGAAECEA-PGGPQGTRHPFDPEIAGS-------HQPGPLI-----HRYW- LOXAF56_2_PE8 NOISO KDFLKTVEGRELQSVKP-SEAPK---ELVIPLIQNGHHRQLLTQTPGPST-----DAET- MACEUGS_741_PE1 NOISO ------------------------------------------------------------ MACMUX_48_PE33 NOISO ------------------------------------------------------------ MICMU15422_PE1 NOISO XXXXXXXXXXXXXXVKP-SEAPK---ELIIPLIQNGHRRQPLAQPPGPSM-----DTGA- MONDO4_393_PE11 NOISO KDFLRAVEGRELHSVRPPAEAPR---ELVIPLLPRGRGRG------------------AA MONDOUN_64_PE2 NOISO -DFLRAVEGRELRSVRPPAEAPR---ELVIPLLPRGRGRG------------------AA MOUSEX_PE58 ENSMUSG00000031148 KDFLATVEGRKLQSVNP-PEAPK---ELVIPLIQNGSRRQPLSKNPKPSS-----ETSTV MOUSEX_PE59 ISOFORMIN ------------------------------------------------------------ NEMVE_9979_PE55 NOISO KDFLHSAERNSLKSVKP-KEEAK---ELVIPLMKKNRWILPT------------------ OCHPRGS_331_PE1 NOISO KDFLKTVEGRQLQXXXX-XXXXX---XXXXXXXXXXXXXXXXXXXXXXXX-----XXXX- ORNANULTRA295_PE13 NOISO KDFLKAVERNEVQSVKP-AEAPK---ELIIPLIKNNQWRKPAPAPAVQ-----KESKPPP ORYLA7_22_PE39 NOISO KDYLTGIHEKELQSSKQ-TEKPK---ELIIPLIQKNRWHKPDRAGQNTEDDGKA-QEKGQ OTOGAGS_386_PE1 NOISO ------------------------------------------------------------ PIGX_44_PE8 NOISO KDFLKTVEGRELQSVKP-SEAPT---ELVIPLIKNGHRRQPTTQAPGPST-----LPEV- PONPYX_50_PE28 NOISO KDFLKTVEGRELQSVKP-QEAPK---ELVIPLIQNGHCRQPPARPPGPST-----DTGA- RABITX_35_PE16 NOISO KDFLKTVEGRQLQSVKP-SEVPK---EPVIPLIQNGYHRQPLVQPSGLST-----DTKT- RATX_PE254 NOISO KDFLATVEGRELQSVNP-PEAPK---ELVIPLIQNGSRRQLLSKNPKPS------ETSTV SORARGS_498_PE1 NOISO --------------VKP-LEAPK---ELVIPLIQNGRQRRQPPAQTQELS-----TDDP- SPETRGS_404_PE1 NOISO ------------------------------------------------------------ TETNG9_PE628 ENSTNIG00000012502 KEYLTRIDKHLLQ---S-SEKPK---ELIIPLIVKNRWNKQGQTE------GNK-EKTAQ TETNG9_PE629 ISOFORMIN KEYLTRIDKHLLQ---S-SEKPK---ELIIPLIVKNRWNKQGQTE------GNK-EKTAQ TRIAD_1217_PE476 NOISO KEIITGLEGNVIEGSKS-SDRNKG--PAVIPMVKRNRWVIPNEKVIANLI-----NQDKS TUPGBGS_385_PE1 NOISO KDFLKTVEGRELQXXXX-XXXXX---XXXXXXXXXXXXXXXXXXXXXXXX-----XXXX- TURTR89660_PE1 NOISO KDFLKTEKRRELQSVQP-SEAPK---ELVIPLIQHGHLRQPPTQAPGPST-----HTEV- XENTR154_2_PE15 NOISO KEYLVGAEGKELLSANP-TPEAK---ELVIPLIHKNRWSKPNKKKDNEEDAVP-----GT MATCH CONSENSUS ???L?????????????????????????IP?????????????????????????????

AILMEGL192563_1_1_PE14 NOISO LMDGV-LSQAVKELIEDSKKSLEERENS------GVDPTLAIPMIQKGCIPN--GEGA-- ANOCA553_PE5 NOISO -INGD-EAQAIRELIEESKKSQEQWESG---NKPD--PAFAIPLLMQNRVPD--GF---E BOVINX_57_PE10 NOISO LMDGM-LSQAVKELIEESKKSLEERENT------GVDPTLAIPMIQKGCTPN--REGT-- CALJAX_48_PE57 NOISO LVDGV-LSQAVKELIEESKKSLEERENA------HVNPTLAIPMIQKGCTPS--GEGA-- CANFAX_43_PE41 NOISO LMDGV-LSQAVKELIEDSKKSLEERENS------GVDPTLAIPMIQKGCIPN--GEGA-- CAVPO132_3_PE19 NOISO LGDGV-LSQAVKELIEESKKSLEERENA------GVDPSLAIPMIQKGCIPG--EEGA-- DANRE8_40_PE13 ENSDARG00000029612 KQDTV-ESQAVKELIEESQKYQDRWKNG---PQSD--PNFAIPLLMQNQVPD--GF---E DANRE8_40_PE14 ISOFORMIN KQDTV-ESQAVKELIEESQKYQDRWKNG---PQSD--PNFAIPLLMQNQVPD--GF---E DASNOGS_505_PE1 NOISO LVDGV-LSQAVKELIXXXXXXXXXXXXX------XXXXXXXXXXXXXXXXXX--XXXX-- DIPORGS_488_PE1 NOISO XXXXX-XX-XXXXXX--XXXXXXXXXXX------XXXXXXXXXXXXXXXXXXXXXXXX-- ERIEUGS_624_PE1 NOISO -VDGV-LSQAVKELIEESKKSLDERENA------GIDPTLAIPMIQKGCTPS--GEWA-- FELCAGS_330_PE1 NOISO LVEGV-LSQAVKEIIEDSKKSLEERENS------GIDPMLAIPMIQKGCIPN--GEGA-- FUGRU226_PE32 ENSTRUG00000000616 DHNSV-DSQAVKELIEDSRRQLEEWQNG---PQPEKLLNLNIPLLMQNKVPE--GF---E FUGRU226_PE33 ISOFORMIN DHNSV-DSQAVKELIEDSRRQLEEWQNG---PQPEKLLNLNIPLLMQNKVPE--GF---E FUGRU226_PE34 ISOFORMIN DHNSV-DSQAVKELIEDSRRQLEEWQNG---PQPEKLLNLNIPLLMQNKVPE--GF---E GASACGROUPXII_17_PE10 ENSGACG00000012127 DNDSV-ESQAVKELIEDSRRQLEEWQNG---SQSEINPNLAIPLLMQNKVPD--GF---E GASACGROUPXII_17_PE11 ISOFORMIN DNDSV-ESQAVKELIEDSRRQLEEWQNG---SQSEINPNLAIPLLMQNKVPD--GF---E GORGOX_52_PE26 NOISO LADGV-LSQAVKELIAESKKSLEERENA------GVDPTLAIPMIQKGCTPS--GEGA-- HORSEX_41_PE12 NOISO LADGM-LSQAVKELIEESKKSLEERENA------GVDPTLAIPMIQKGCTPS--GEGA-- HSX_PE902 ENSG00000068394 LADGV-VSQAVKELIAESKKSLEERENA------GVDPTLAIPMIQKGCTPS--GEGA-- HSX_PE903 ISOFORMIN GLDGV-VSQAVKELIAESKKSLEERENA------GVDPTLAIPMIQKGCTPS--GEGA-- LOXAF56_2_PE8 NOISO LVDGVLLSQAVKELIEESKKSLEERENE------GVDPTLAIPMIQKGCIPS--GEGA-- MACEUGS_741_PE1 NOISO --------QAEKELTTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--------- MACMUX_48_PE33 NOISO ------------------------------------------------------------ MICMU15422_PE1 NOISO LVDGV-LSQAVKEIIEESKKSLEERENA------GIDPTLAIPMIQKGCTPN--REGA-- MONDO4_393_PE11 NOISO PEDAV-LCQAVQELIEESQRALQQPS----DGAEAADPSLAIPLLARAGAE--------- MONDOUN_64_PE2 NOISO PEDAV-LCQAVQELIEESQRALQQPS----DGAEAADPSLAIPLLARAGAE--------- MOUSEX_PE58 ENSMUSG00000031148 LMSDGVLSQAVKELIEESKKSLEERENA------GVDPTLTIPMIQKGCTPI--EEGS-- MOUSEX_PE59 ISOFORMIN -------------------------------------------MIQKGCTPI--EEGS-- NEMVE_9979_PE55 NOISO -VEGL-DKQAMEEVIKEAQKDLQSKE--------SEEGDLTIPILMRNKLPD--ISGSDK OCHPRGS_331_PE1 NOISO XXXXX-X--XXXXXXXXXXXXXXXXXXX------XXXXXXXXXXXXXXXXXXXXXXXX-- ORNANULTRA295_PE13 NOISO PEDGV-LTQAVKEIIEESKKSLEERENG-----SQVDPNLAIPLLMQNRVPD--GF---E ORYLA7_22_PE39 NOISO DSDSV-ESQAVKELIEDSRRQLEQWQNG---SESEKNLNLSIPLLMQNKVPE--GF---E OTOGAGS_386_PE1 NOISO ------------------------------------------------------------ PIGX_44_PE8 NOISO LADGM-LSQAVKEIIEESKKSLEERENA------GVDPTLAIPMIQKGCTPAHPGQGA-- PONPYX_50_PE28 NOISO LADGV-LSQAVKELIEESKKSLEERENA------GVDPTLAIPMIQKGCTPS--GEGA-- RABITX_35_PE16 NOISO LMDEV-LSQAVKELIEETEKSLEERKNA------NADSMLTIPMIQKGYTSN--KEGV-- RATX_PE254 NOISO LTSDGVLSQAVKELIEESKKSLEERENA------GVDPTLTIPMIQKGCTPI--GEGT-- SORARGS_498_PE1 NOISO MMDGV-LSQAVKELIEESKKSLEERENA------GVDPTLAIPMIQKGCTSS--ERGE-- SPETRGS_404_PE1 NOISO ------------------------------------------------------------ TETNG9_PE628 ENSTNIG00000012502 DSDSV-DSQAVKELIEDSRRQLDQWQNG---SQPEDVLNLNIPLLMQNKVPD--GF---E TETNG9_PE629 ISOFORMIN DSDSV-DSQAVKELIEDSRRQLDQWQNG---SQPEDVLNLNIPLLMQNKVPD--GF---E TRIAD_1217_PE476 NOISO NLD----AEAAQEILQEINSQVE---VN------DAEEEHIVPLLMQNCAPRVKGIES-- TUPGBGS_385_PE1 NOISO XXXXX-XXXXXXXXX--XXXXXXXXXXX------XXXXXXXXXXXXXXXXXXXXXXXX-- TURTR89660_PE1 NOISO LVDGM-LSQAVKELIEESTKSLEERENA------GVDPTLAIPMIRKGCTPI--GEGA-- XENTR154_2_PE15 NOISO EEEAV-LSQAVKELIEESRRTLEDN--------SETDQTLSIPLLMQNRIPD--GY---E MATCH CONSENSUS ????????QA??E?I???????????????????????????P?????????????????

AILMEGL192563_1_1_PE14 NOISO -DSEPRAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP ANOCA553_PE5 NOISO DGEKVDVSLRPES---ADYEEVPVEAYGFAMLKGMGWKAGEGIGRTFKQDVKPLENRLRP BOVINX_57_PE10 NOISO -DSEPQAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFSQVVKPRVNSLRP CALJAX_48_PE57 NOISO -DSEPRAET---VPEEANYEVVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP CANFAX_43_PE41 NOISO -DSEPRAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNLVVKPRLNLLRP CAVPO132_3_PE19 NOISO -DSTPRAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNLLRP DANRE8_40_PE13 ENSDARG00000029612 DGDKVNVDLRPESSTEADYERVPVEAYGLAMLKGMGWKQEEGIGRTFKQDVKPIEHQLRP DANRE8_40_PE14 ISOFORMIN DGDKVNVDLRPESSTEADYERVPVEAYGLAMLKGMGWKQEEGIGRTFKQDVKPIEHQLRP DASNOGS_505_PE1 NOISO -XXXXXXXX---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVKPHVNSLRP DIPORGS_488_PE1 NOISO -XXXXXXXX---VPKEAVYEVVPVEAYGMSMLRGMGWKPGKGIGR-LNPVVKLQNHSVRP ERIEUGS_624_PE1 NOISO -DKEPQADT---KPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNALRP FELCAGS_330_PE1 NOISO -DSEPRAET---XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVVKPRVNSLRP FUGRU226_PE32 ENSTRUG00000000616 DGDHIKVDLRPESSAEADYEQVPVEAYGYAMIRGMGWNKSEGIGRTFQQHVTAVEHQVRP FUGRU226_PE33 ISOFORMIN DGDHIKVDLRPESSAEADYEQVPVEAYGYAMIRGMGWNKSEGIGRTFQQHVTAVEHQVRP FUGRU226_PE34 ISOFORMIN DGDHIKVDLRPESSAEADYEQVPVEAYGYAMIRGMGWNKSEGIGRTFQQHVTAVEHQVRP GASACGROUPXII_17_PE10 ENSGACG00000012127 DGDRVKVDLRPESSTEADYESIPVEAYGLAMIKGMGWNKEEGIGRTFKQHVTPIEHELRP GASACGROUPXII_17_PE11 ISOFORMIN DGDRVKVDLRPESSTEADYESIPVEAYGLAMIKGMGWNKEEGIGRTFKQHVTPIEHELRP GORGOX_52_PE26 NOISO -DSEPQAET---VPEEANYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP HORSEX_41_PE12 NOISO -DSERWAET---VPEEADYEAVPVEAYGLALLRGMGWKPGEGIGRTFNQVVKPRVNSLRP HSX_PE902 ENSG00000068394 -DSEPRAET---VPEEANYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP HSX_PE903 ISOFORMIN -DSEPRAET---VPEEANYEAVPVEAYWPCCG---AWAGNLAHRPHLQSSSEAPCQLTEA LOXAF56_2_PE8 NOISO -DSEPQAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFSQVVKPRVNSLRP MACEUGS_741_PE1 NOISO ------XXXXXXXSVGADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFKQVVKPRENPLRP MACMUX_48_PE33 NOISO ------------VPEEANYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP MICMU15422_PE1 NOISO -DSEPQAET---MPEEADYESVPVEAYGMAMLRGMGWKPGKGIGRTFNQVVKPRVNSLRP MONDO4_393_PE11 NOISO ------TEAAAAPSVGADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFKQVVKPRENPLRP MONDOUN_64_PE2 NOISO ------TEAAAAPSVGADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFKQVVKPRENPLRP MOUSEX_PE58 ENSMUSG00000031148 -DSEPQAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGKGIGNTFSQVVKPRVNSIRP MOUSEX_PE59 ISOFORMIN -DSEPQAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGKGIGNTFSQVVKPRVNSIRP NEMVE_9979_PE55 NOISO DAVKMDIALRPEPTTLEEYEAMPVSAFGAAMLRGMGWKKGQAIGGSVKGLAEPIEYIPRS OCHPRGS_331_PE1 NOISO -XXXXXXXX---VPEEADYEAISVEAYGLAMLRGMGWKPGQGIGRTFSQVVKPRVNSLRP ORNANULTRA295_PE13 NOISO DGDKKET-QRTQVATEADYEVVPVEAYGIAMLRGMGWKAGEGIGRTFKHKTVQPPRAMAG ORYLA7_22_PE39 NOISO DGDLINVGLRPESSTEADYESVPVEAYGLAMLKGMGWKKSEGIGRTFKQDVKPIEHQLRP OTOGAGS_386_PE1 NOISO --------------------------------------------RTFNQVVKPVNT--LR PIGX_44_PE8 NOISO -DSEPRAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP PONPYX_50_PE28 NOISO -DSEPRAET---VPEEANYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP RABITX_35_PE16 NOISO -DSEHSAET---VPKEADYEAVSVEAYGLAMLRGMGWKPGDGIGRTFSQIVKPRVNSLRP RATX_PE254 NOISO -YSEPQAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGKGIGNTFSQVVKPRVNSIRP SORARGS_498_PE1 NOISO -DSEPQAET---TPEEANYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP SPETRGS_404_PE1 NOISO -------------------------------------------------VVKPRVNSLRP TETNG9_PE628 ENSTNIG00000012502 DGDHVKVDLRPESSAEADYEQVPVEAYGYAMIRGMGWKKSEGIGRTFKQNVTAVEHQVRP TETNG9_PE629 ISOFORMIN DGDHVKVDLRPESSAEADYEQVPVEAYGYAMIRGMGWKKSEGIGRTFKQNVTAVEHQVRP TRIAD_1217_PE476 NOISO -SEKLDIAMRPETSSLEDYENMPISNFGQAMLRGMGWSVGQGIGKTNKQVIKPIEFTPRY TUPGBGS_385_PE1 NOISO -XXXXXXXX---VPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRP TURTR89660_PE1 NOISO -DSEPRAET---VPEEADYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVTSLRP XENTR154_2_PE15 NOISO DGEKVDVSLRPDSAEAADYDAVPVEYYGMAMLRGMGWKEGEGIGRTFKQDVKPLEQKLRP MATCH CONSENSUS ????????????????A?YE?VPVEAYG?AM??GMGW????GIGRTF?Q?V?P?????RP

AILMEGL192563_1_1_PE14 NOISO KGLG--LGANLNEVQA--LVSTGPHHLPKPDEEQE---------------------KEKE ANOCA553_PE5 NOISO KGLG--LGADRSAAQE--LEPIGRRRPLKPGEE----------------------PPKGK BOVINX_57_PE10 NOISO KGLG--LGANLTEVQAQAQAPASPYRLPRPDEEQE---------------------KDKE CALJAX_48_PE57 NOISO KGLG--LGANLTEAQT--LTPTGPSHMPRPDE-QG---------------------KDKE CANFAX_43_PE41 NOISO KGLG--LGANLNEVQA--LVPTGPHHLPKPDEEQE---------------------KAKE CAVPO132_3_PE19 NOISO KGLG--LGASLTEAQA--LPSSGSYCPPKPGGEQK---------------------KGKE DANRE8_40_PE13 ENSDARG00000029612 KGLG--LGADRSAIKD--LEPGVPKRPPKPGDE----------------------KGKEE DANRE8_40_PE14 ISOFORMIN KGLG--LGADRSAIKD--LEPGVPKRPPKPGDE----------------------KGKEE DASNOGS_505_PE1 NOISO KGLG--LGANLAVTQA--LATTSPYRLPRPGEEQE---------------------KEKE DIPORGS_488_PE1 NOISO KGLG--LGVNLTEARA--LASIGSHHSSRPGGEQQ---------------------KDKD ERIEUGS_624_PE1 NOISO KGLG--LGANLAEVQD--LAPPGSQRLLKTD-EQE---------------------KDKE FELCAGS_330_PE1 NOISO KGLG--LGANLTEVQA--LAPTGPHRLPKPDEEQE---------------------KDKE FUGRU226_PE32 ENSTRUG00000000616 KGLG--LGADRSLIKD--LEPSKRKQPPKPGDE----------------------RVKEE FUGRU226_PE33 ISOFORMIN KGLG--LGADRSLIKD--LEPSKRKQPPKPGDE----------------------RVKEE FUGRU226_PE34 ISOFORMIN KGLG--LGADRSLIKD--LEPSKRKQPPKPGDE----------------------RVKEE GASACGROUPXII_17_PE10 ENSGACG00000012127 KGLG--LGADRSAIKD--LEPTRHKRPPKPGEE----------------------REKEE GASACGROUPXII_17_PE11 ISOFORMIN KGLG--LGADRSAIKD--LEPTRHKRPPKPGEE----------------------REKEE GORGOX_52_PE26 NOISO KGLG--LGANLTEAQA--LTPTGPSRMPRPDEEQE---------------------KDKE HORSEX_41_PE12 NOISO KGLG--LGANLTEVQA--LAPTGPHRLPRPDEEQE---------------------KDKE HSX_PE902 ENSG00000068394 KGLG--LGANLTEAQA--LTPTGPSRMPRPDEEQE---------------------KDKE HSX_PE903 ISOFORMIN QGVR--AGC---QPDRQALTPTGPSRMPRPDEEQE---------------------KDKE LOXAF56_2_PE8 NOISO KGLG--LGADLTKAKA--LASTGPHRLPKPDE-QE---------------------KEKE MACEUGS_741_PE1 NOISO KGLG--LGASLVLP----------SGPPGPPAR----------------------EPKDD MACMUX_48_PE33 NOISO KGLG--LGANLSEAQA--LTPTGPSHMPRPDEEQE---------------------KDKE MICMU15422_PE1 NOISO KGLG--LGANLTEAQA--LTPTSSSHVPRPDKEQE---------------------KDKE MONDO4_393_PE11 NOISO KGLG--LGASLAPS----------PAP-APPG-----------------------NKRAS MONDOUN_64_PE2 NOISO KGLG--LGASLAPS----------PAP-APPG-----------------------NKRAS MOUSEX_PE58 ENSMUSG00000031148 KGLG--LGANRMEAQD--LASVGSHHPPRPDGDRE---------------------NDKE MOUSEX_PE59 ISOFORMIN KGLG--LGANRMEAQD--LASVGSHHPPRPDGDRE---------------------NDKE NEMVE_9979_PE55 NOISO KGLG--LGAEVRAEM-----PSKRRR--KPGDKDQKHSGPIVGKDGRVRHVKGVGEEVKP OCHPRGS_331_PE1 NOISO KGLG--LGANVTDVQALT-TSTSSSCLSRTHH------------------------SQKD ORNANULTRA295_PE13 NOISO DGKGCWTGAKGWGVLG--VPYTRSHFPPHPQVG----------------------AGAKS ORYLA7_22_PE39 NOISO KGLG--LGADRSAIKD--LEPNRNQRPPKPGDE----------------------PGKEE OTOGAGS_386_PE1 NOISO KGLG--LGANLTEAT---LAPTGSSHVPRPDEKPD---------------------KDKE PIGX_44_PE8 NOISO KGLG--LGANLTEVQA--LAPSSPHRLPRPDEERE---------------------KDKE PONPYX_50_PE28 NOISO KGLG--LGANLTEAQA--LT-TGPSRMPRPDEEQD---------------------KDKE RABITX_35_PE16 NOISO KGLG--LGANLAEMKA--LTSTSSSCLPRTNVDQDQDD-----------------GQDTT RATX_PE254 NOISO KGLG--LGANLMETQA--LASTGSHHPPRPDGDRE---------------------KDKE SORARGS_498_PE1 NOISO KGLG--LGASLTDLQS--LAPIGPHRPPKPNEERE---------------------KDKE SPETRGS_404_PE1 NOISO KGLG--LGANLTEAQA--LASTGSNTLLRPDGEQE---------------------KDKE TETNG9_PE628 ENSTNIG00000012502 KGLG--LGADRSLIKD--LEPSKRKQPPKPGDE----------------------RTQEE TETNG9_PE629 ISOFORMIN KGLG--LGADRSLIKD--LEPSKRKQPPKPGDE----------------------RTQEE TRIAD_1217_PE476 NOISO KGLG--LGAERLVESDD-KNKNKPDKIRKPGEKYEEPER--------TSDV-CFAAKMVT TUPGBGS_385_PE1 NOISO KGLG--LGANLTETQA--LAPTGSSRLSKPDGEPE---------------------KDKE TURTR89660_PE1 NOISO NGLG--LGANMTEFQA--LAPTGPHHLLRPDEEQE---------------------KDKE XENTR154_2_PE15 NOISO KGLG--LGADRSALKD--LEPQKPRKPLKPGEK----------------------LEE-- MATCH .* * . CONSENSUS KGLG??LGA?????????L??????????P??????????????????????????????

AILMEGL192563_1_1_PE14 NOISO DQPQGLVPGGAVMVLSGPHR--GLYGKIEGLDPD----N--VRAMVRLAVGNRMVTVSEY ANOCA553_PE5 NOISO DEPLGLVPGGAVFIEAGPHK--ELYGKIEGVDGD----N--ARIMVKLAIGGKTVTVSQH BOVINX_57_PE10 NOISO DQPKGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGSRMVTVSEY CALJAX_48_PE57 NOISO DQPQGLVPGGAVMVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGSRMVTVSEY CANFAX_43_PE41 NOISO DQPQGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGNRMVTVSEY CAVPO132_3_PE19 NOISO DQPQGLVPGRTVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLALGSRMVTVSEY DANRE8_40_PE13 ENSDARG00000029612 -EALVLGPGGCVQVLAGAHK--DLYGKIEGVDPD----N--GRVVVKLAIGGKTVTIIQH DANRE8_40_PE14 ISOFORMIN -EALVLGPGGCVQVLAGAHK--DLYGKIEGVDPD----N--GRVVVKLAIGGKTVTIIQH DASNOGS_505_PE1 NOISO DQPQGLVPGGAVVILSGPYR--GLYGKVEGLDPD----N--VRAMVRLAMVSRVVTVIEY DIPORGS_488_PE1 NOISO IEPQGLVIGGAVLIFSGPHR--GLYGKVEGIDPD----N--VRAMVRLAESSRIVTVSEY ERIEUGS_624_PE1 NOISO DQPKGLVPGGAVLVLSGPHR--GLYGKVEGLDPD----N--ARAMVRLASGSRVVTVSEY FELCAGS_330_PE1 NOISO DQPQGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----S--VRAMVHLAMGSRMVTVSEY FUGRU226_PE32 ENSTRUG00000000616 --ELVMGPGSCVLVESGPHK--DKYGKIEGVYPD----N--ARVVVRLAIGGRTVDISQY FUGRU226_PE33 ISOFORMIN --ELVMGPGSCVLVESGPHK--DKYGKIEGVYPD----N--ARVVVRLAIGGRTVDISQY FUGRU226_PE34 ISOFORMIN --ELVMGPGSCVLVESGPHK--DKYGKIEGVYPD----N--ARVVVRLAIGGRTVDISQY GASACGROUPXII_17_PE10 ENSGACG00000012127 --ELVMGPGGCILVESGAHK--DMYGKIEGVDAD----N--ARVVVKLAIGGRSATISQY GASACGROUPXII_17_PE11 ISOFORMIN --ELVMGPGGCILVESGAHK--DMYGKIEGVDAD----N--ARVVVKLAIGGRSATISQY GORGOX_52_PE26 NOISO DQPQGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLSVGSRVVTVSEY HORSEX_41_PE12 NOISO DQPQGLVPGGAVVVRSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGSRVVTLSEY HSX_PE902 ENSG00000068394 DQPQGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGSRVVTVSEY HSX_PE903 ISOFORMIN DQPQGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGSRVVTVSEY LOXAF56_2_PE8 NOISO DQPQGLVTGGAVVVLSGPHR--GIYGKVEGFDPD----N--VRAMVRLAAGSRVVTVSEY MACEUGS_741_PE1 NOISO EGLVGLAPGRAVVVLSGPHR--GLYGQVQGLDPD----N--ARAVVKLAPAGHVATLSEH MACMUX_48_PE33 NOISO DQPQGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGSRVVTVSEY MICMU15422_PE1 NOISO DQPEALVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAAGSRV-TVSEY MONDO4_393_PE11 NOISO EEPEGLAPGRAVLVLAGPHR--GLYGKVEGLDPD----N--ARAVVRLAASGQAATLSEY MONDOUN_64_PE2 NOISO EEPEGLAPGRAVLVLAGPHR--GLYGKVEGLDPD----N--ARAVVRLAASGQAATLSEY MOUSEX_PE58 ENSMUSG00000031148 GQPQGLMHGRAVVVLSGPYR--GLYGKVEGLDPD----N--VRAMVRLAVGNRIVTVSEY MOUSEX_PE59 ISOFORMIN GQPQGLMHGRAVVVLSGPYR--GLYGKVEGLDPD----N--VRAMVRLAVGNRIVTVSEY NEMVE_9979_PE55 NOISO SEPLDYRVGTCAVIGRGAHK--DLHGKIISVDVD----N--SRVTVSLHISNENITLHQY OCHPRGS_331_PE1 NOISO EQPDGLKNGTTVMVISGHYR--GRYGKVEGLDPD----N--VRAMVRLSRENRVVTVSEY ORNANULTRA295_PE13 NOISO KESR---PAGVTGQECGPAGVTVSLPFLQGIFPDLSASNYSAVILILLSSGSQLIPITEP ORYLA7_22_PE39 NOISO --ELVMGPGGCVLVESGAHK--DLYGKIEGVDAD----N--ARVVVKLAVGGKTVTLSQH OTOGAGS_386_PE1 NOISO DKPQTLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLVVGNRMVTVSEY PIGX_44_PE8 NOISO DQPRGLVPGGAVVVLSGPHR--GLYGKIEGLDPD----N--VRAMVRLAVGSRMVTVSEY PONPYX_50_PE28 NOISO DQPQGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGNRVVTVSEY RABITX_35_PE16 NOISO RQPEGLKTGTAVTVVSGHYR--GRYGKVEGLDPD----N--ARAMVRLTGENSVVTVSEY RATX_PE254 NOISO GQPQGLMHGGAVVVLSGPYR--GLYGKVEGLDPD----N--VRAMVRLAVGNRVVNVSEY SORARGS_498_PE1 NOISO EQPKELVPGGAVVMLSGPHR--GLYGKVEGLDPD----N--ARAMVRLATKSHMVTVSEY SPETRGS_404_PE1 NOISO DQPQGLVPGGAVMVLSGPHR--GLYGKXXXXXXX----X--XXXXXXXXXXXXXXXXXXX TETNG9_PE628 ENSTNIG00000012502 --ELVMAPGSCVLVESGPHK--DKYGKIEGVYPD----N--ARVVVKLAIGGKTVDISQY TETNG9_PE629 ISOFORMIN --ELVMAPGSCVLVESGPHK--DKYGKIEGVYPD----N--ARVVVKLAIGGKTVDISQY TRIAD_1217_PE476 NOISO TISLGFARGVHVMVVAGPHA--DLYGKVEGIDGD----N--GRIFVALAINKEKTTISQN TUPGBGS_385_PE1 NOISO DQPQGLVPGGTVVVLSGPHR--GLYGKVEGLDPD----N--VRAMVRLAVGSRV-TVSEY TURTR89660_PE1 NOISO DQPQGLVPGGAVVVLSGPHR--GLYGKVEGLDPD----N--ARAMVRLAVGSRVVTVSEY XENTR154_2_PE15 NOISO -ESRGLGKGSSVQIQSGAHK--DLYGKVEGVDPD----N--SRAMIKLAIGGKVVTVSLF MATCH . * CONSENSUS ????????G??V?V?SGPH?????YGK?EG?DPD????N???R??V?LA??????T?S?Y

AILMEGL192563_1_1_PE14 NOISO CLRPISQKEFDKNS--LGLSQMSKTS----P--G------QQ---NGTALSWKALRDQ-D ANOCA553_PE5 NOISO GVRPVTQKEYDKLAKDLSRLSKAHKEKEAEQRNGTKLDS------DNR-----------V BOVINX_57_PE10 NOISO CLRPVSQKEFDKN---LNLSQVSRTS----P--G------EH---HRTTSPRKTLRDQ-D CALJAX_48_PE57 NOISO CLRPVSQQEFDKNA--LDLRQ--------------------Q---NRTASLQKTLRNQ-E CANFAX_43_PE41 NOISO CLRPVSQHEFDKNT--LDLSQMNKTS----P--G------QQ---NGTASSWKALRDQ-D CAVPO132_3_PE19 NOISO CLRPVSQQEFDKNLHSDKIGQASRTS----L--G------QH---NGSSLSRKAHQSE-D DANRE8_40_PE13 ENSDARG00000029612 SIKLVTRKEYDKYSKDLSRLSKAHKDKEREK-EREQKREQEQR-LNGKDERG-----KTD DANRE8_40_PE14 ISOFORMIN SIKLVTRKEYDNTAKTSVVLVKHIKTRKEKK-NG-NK--------RGNRSRG-------- DASNOGS_505_PE1 NOISO CLRPVSQKEFDKNSLEI-------------------------KQQNGTVSSCKALRNQ-E DIPORGS_488_PE1 NOISO CLKPVSQKEFDSNTLHTEQ--------------------------HNETSSVKADQNQ-D ERIEUGS_624_PE1 NOISO CLRPISQKEFDKKSSD--------------P--R------DQ---NGTA-TSKAQRGQ-E FELCAGS_330_PE1 NOISO WL-PVSQQEFDKNSL--------------------------------------------- FUGRU226_PE32 ENSTRUG00000000616 IVKLVGRKEYDRYSKDLSKKLSEMHHIK-AEKVKDPKRCEEKERSDGHEG-K-----HNS FUGRU226_PE33 ISOFORMIN IVKLVGRKEYDRYSKDLS------HFSK-AEKVKDPKRCEEKERSDGHEG-K-----HNS FUGRU226_PE34 ISOFORMIN IVKLVGRKEYDRYSKDLSKPTQGTRNAG-RGPLV-------------------------- GASACGROUPXII_17_PE10 ENSGACG00000012127 AIKLVGRKEYDKNGKDYMLTTKCHTNDFSSENVRSLARCYSLIFFGQRKRKC-----CR- GASACGROUPXII_17_PE11 ISOFORMIN AIKLVGRKEYDKNGKDLIEVLKNKHKSAIRAK---------------------------- GORGOX_52_PE26 NOISO YLRPVSQQEFDKNT--LDLRQ--------------------Q---NGTASSRKTLWNQ-E HORSEX_41_PE12 NOISO YLQPVSQQEFDKNS--MELRQ--------------------Q---NGTASSRKALRDQ-D HSX_PE902 ENSG00000068394 YLRPVSQQEFDKNT--LDLRQ--------------------Q---NGTASSRKTLWNQ-E HSX_PE903 ISOFORMIN CGLSPSRSLTRTPW----------------------------ISGNRTELPHKTLWNQ-E LOXAF56_2_PE8 NOISO CLRPISQQEFDKNS--SDLGQVSKTS----S--E------QQ---NRTASSWKALRDQ-D MACEUGS_741_PE1 NOISO VLRPVSRQEYDRQTLK-------------------------------------------- MACMUX_48_PE33 NOISO CLRPVSQQEFNKNT--LDLRQ--------------------Q---NGTASSRKTLRNQ-E MICMU15422_PE1 NOISO CLRSVSQQEFDN-S--LDLRQ--------------------Q---NGTASSRKALRDQ-D MONDO4_393_PE11 NOISO VLRPVSCQEYEKQAKGPGRPPGDPQA-------------------GGAGQ---------- MONDOUN_64_PE2 NOISO VLRPVSCQEYEKQAKGPGRPPGDPQP-------------------GGAGQ---------- MOUSEX_PE58 ENSMUSG00000031148 CLRPVSQQEFDSHT--SKPGHVSQTS----T--E------QQNRATGTASSLKAAQNQED MOUSEX_PE59 ISOFORMIN CLRPVSQQEFDSHT--SKPGHVSQTS----T--E------QQNRATGTASSLKAAQNQED NEMVE_9979_PE55 NOISO HVRLIDKDEYKELCQEGKSRSKSNKDKESSKDRD-----------NSHRS---------- OCHPRGS_331_PE1 NOISO CLRPVSQKEYDENTADPR--KL-----------------------RQPPSTQKAPQDQ-H ORNANULTRA295_PE13 NOISO ASRPLSGPEARLS-----RPGPELQNG-IDQR-----------LRGGSPS-I-----RAA ORYLA7_22_PE39 NOISO GVRLVGRKEYDKYSKDISDFSFIHVGRIKHKRIRQCVLSFICFLATGRLSKA-----RRA OTOGAGS_386_PE1 NOISO CLRAVSQQEFDKNS--LDLRQ--------------------E---NGTTSSRKALQNQ-D PIGX_44_PE8 NOISO CLRPVSQQEFDKNS--LDLGQVSQTS----P--E------RQ---NGTASSWKALRDQ-D PONPYX_50_PE28 NOISO CLRHVSQQEFDKNT--LDLS---------------------------------------- RABITX_35_PE16 NOISO CLRPVSQQEHDENSMDLN--QENTTP----T--G------RP---TDPALSQKTLQDQG- RATX_PE254 NOISO CLRPVSQQEFDSHT--SKPGHVSKTS----T--E------QQHRATGTASSVKALQNQED SORARGS_498_PE1 NOISO CLRPVSQQEFDKNTNPG-----------------------QL---NGKGS-------Q-D SPETRGS_404_PE1 NOISO XXX-------------XXXXXXXXXX----X--X------XXXQQNGTGSSRKALQNQ-D TETNG9_PE628 ENSTNIG00000012502 IVRLVGRKEYDRYSKDLSHFSKAEKVKE-KEREKDAKRHEERERSDGHSS---------- TETNG9_PE629 ISOFORMIN IVRLVGRKEYDRYSKDLSHFSKE--------------RHEERERSDGHSS---------- TRIAD_1217_PE476 NOISO CLKIVSEKEYTKNCMRLNKVDKVQSFINSRS--G------REETLNSTDSLKSSKRKRGD TUPGBGS_385_PE1 NOISO YXXXXXXXXXX----------XXXXX----X--X------QQ---NGAALSWKGVRNQ-D TURTR89660_PE1 NOISO CLRPVSQQEFDKNS--LDLRQ--------------------Q---NGTASSWKALQDQ-D XENTR154_2_PE15 NOISO SLRLVSSAEYTKFAKDLSECNCNKVCCTISK----------------------------- MATCH CONSENSUS ????V???E???????????????????????????????????????????????????

AILMEGL192563_1_1_PE14 NOISO LPGQREDSERKRKHLPDRQGEP-------AAKNEK-AGPWSQHWLHRDLRVRFVDKQHKG ANOCA553_PE5 NOISO EEDKKENRDKK---------RKHPAGTDRPTEIENCSSSSVPHWLRRDLRVRFVDKLYKG BOVINX_57_PE10 NOISO SHICWEDAERKRKHHPDRQDGP-------ATKNEK-AAPRSQHWLHRDLRVRFVDKLHKG CALJAX_48_PE57 NOISO LHIQQDNSERKRKHLPGRQNGL-------TAKIEK-AAPRSRHWLHRDLRVRFVDKMYKG CANFAX_43_PE41 NOISO LPSWREDSERKRKHLPDRQGEP-------AAKNEK-AGPQSQHWLHRDLRVRFVDRQHKG CAVPO132_3_PE19 NOISO LRGRQEDPEKKRKHPPDRQDGP-------VAKSEK-AASRSQHWLHRDLRVRFVDKLYKG DANRE8_40_PE13 ENSDARG00000029612 RSREKDRDQKKRKHRESSTDRE-----KPPPAKESRSSPPAPSWLQRDLRVRFIDKAFKG DANRE8_40_PE14 ISOFORMIN ------------------------------------------------------------ DASNOGS_505_PE1 NOISO LHVRWEDSERKRKHPPDRQDGP-------AAKSEK-ATPRSQHWLRRDLRVRFVDKLHKG DIPORGS_488_PE1 NOISO SQVHQ-ENSEKRKYPADRQDGP-------AAKNEK-VASRSHHWLHRDLRVRFVDKLYKG ERIEUGS_624_PE1 NOISO LHVRWEDTERKRKHLPDXXXXX-------XXXXXXXXXXXXXX-XXXXXXXXXXXXXXXX FELCAGS_330_PE1 NOISO ------------------------------------------------------------ FUGRU226_PE32 ENSTRUG00000000616 SEKHQRKDEKKRKQRESSPDRSIPIKREKHPEKETRKPPAPPSWLQRDLKVRFIDRDFKG FUGRU226_PE33 ISOFORMIN SEKHQRKDEKKRKQRESSPDRSIPIKREKHPEKETRKPPAPPSWLQRDLKVRFIDRDFKG FUGRU226_PE34 ISOFORMIN -------PDTFSRNNNTSLKSVLVFYREKHPEKETRKPPAPPSWLQRDLKVRFIDRDFKG GASACGROUPXII_17_PE10 ENSGACG00000012127 GLREIEVLKNKHKHRESSQ------DREKPPVKEARRKPAPPSWLQRDLKVRFIDKAFKG GASACGROUPXII_17_PE11 ISOFORMIN ------------------------------------------------------------ GORGOX_52_PE26 NOISO LHIQQDNSERKRKHLPDRQDGP-------AAKSEK-AAPRSQHWLHRDLRVRFVDNMYKG HORSEX_41_PE12 NOISO LHIRQEDSERKRKHLPDRQDGP-------AAKSEK-AAPRSQHWLHRDLRVRFVDKLHKG HSX_PE902 ENSG00000068394 LYIQQDNSERKRKHLPDRQDGP-------AAKSEK-AAPRSQHWLHRDLRVRFVDNMYKG HSX_PE903 ISOFORMIN LYIQQDNSERKRKHLPDHRMGL-------QPRVRKQPPEVS------------------- LOXAF56_2_PE8 NOISO LRVRQEDSERKRKHPPDREDGP-------AAKSEK-AAPRSQHWLRRDLRVRFVDKLHKG MACEUGS_741_PE1 NOISO ------------------------------EGAEKEAAPRSGHWLRRDLRVRFVDKGHYK MACMUX_48_PE33 NOISO LHVQQDNSERKRKHLPDRLDGP-------AAKSEK-AAPRSQHWLHRDLRVRFVDKMYKG MICMU15422_PE1 NOISO LHVQQENSERKRKHLPNRQDGP-------AAKSGK-AAPRSQHWLHRDLRVRFVNKLHKV MONDO4_393_PE11 NOISO --------------------------GAKKKGQKRKQPPTTPHWLRRDLRVRFVDKRHHK MONDOUN_64_PE2 NOISO --------------------------GAKKKGQKRKQPPTTPHWLRRDLRVRFVDKRHHK MOUSEX_PE58 ENSMUSG00000031148 SKRRQKGSEKKRKHSPDRQDGP-------VPKTEK-AAPRNKHWLHRDLRVRFIDKLHKS MOUSEX_PE59 ISOFORMIN SKRRQKGSEKKRKHSPDRQDGP-------VPKTEK-AAPRNKHWLHRDLRVRFIDKLHKS NEMVE_9979_PE55 NOISO TKRRKE----ENTHEKSSG---SYKQKSKHDKSEVNGEKKKKCWLHPELRVRIISKDYKK OCHPRGS_331_PE1 NOISO ARTQQQSSMKKRKHLVDPQEGP-------TTKSSEKTARRSPHWLRRDLRVRFVNKLYKN ORNANULTRA295_PE13 NOISO GAKSKES----RPAGVTGQ-EGGPAG-VTGKEGGPAGSSRAPHWLQRDLRVRFVDKLYKA ORYLA7_22_PE39 NOISO SEKDEPKEERKRKHKESNQ------DRDKPPVKEARQAAAPPSWLQRDLKVRFIDKAFKG OTOGAGS_386_PE1 NOISO LHVQRENCEKKRKHLPDRHDEP-------AAKGEK-AAPRRQHWLHRDLHVRFVDKLHKG PIGX_44_PE8 NOISO LHVRREDSERKRKHLPDRPDGP-------AAKSEK-AAPRSQHWLHRDLRVRFVDKQHKG PONPYX_50_PE28 NOISO ------------------------------------------------------------ RABITX_35_PE16 NOISO -AHAHLDSTRKRKHPVDQQEGP-------VAKSEK-TASRSPHWIHRDLRVRFVNKLYKN RATX_PE254 NOISO SKRRQKDSEKKRKHSPDRRDGP-------VPKTEK-AAPRNKHWLHRDLRVRFIDKVHKG SORARGS_498_PE1 NOISO -LDLWEDSHRKRKHLPDHQSGP-------SAKGNTATASRTQHWLHRDLRVRFVDKQHKG SPETRGS_404_PE1 NOISO LHVRWEDSERKRKHPPDRNDGP-------AAKNKK-ATSMTQHWLHRDLRVRFVDKLHKG TETNG9_PE628 ENSTNIG00000012502 SEKDWRKEEKKRKHGDLSQDKHIPNKREKHLEKDTRKPAAPPSWLQRDLKVRFIDKDFKG TETNG9_PE629 ISOFORMIN SEKDWRKEEKKRKHGDLSQDKHIPNKREKHLEKDTRKPAAPPSWLQRDLKVRFIDKDFKG TRIAD_1217_PE476 NOISO -EERYPSEDKNRSHHGNNYTGS-----KDHYHSNKHDETGSNVWVRPKTRVRLISKSYKH TUPGBGS_385_PE1 NOISO LHVRWEDSEKKRKHFPDRQDGP-------AAKTEK-AAPRSQHWLHRDLRVRFVDKLHKG TURTR89660_PE1 NOISO LHVRQEDSARKRKHHPDRQDEP-------AAKSEK-AAPRSQHWLHRDLRVRFVDKLHKG XENTR154_2_PE15 NOISO ------------------------------------------------------------ MATCH CONSENSUS ???????????????????????????????????????????WL?RDL?VRF???????

AILMEGL192563_1_1_PE14 NOISO GQYYNTKMTIEDVLSLDTCVCRTDEGRILEGVKEGMLETLVPKVEGNRVMVVLGPQAGRT ANOCA553_PE5 NOISO GKYYNTKMVIEDVLKPDTCVCRTEEGRILDGILESMLETVIPRSDSDQVMVVLGEHSGR- BOVINX_57_PE10 NOISO GQYYNTKMTIEDVLSPDTCVCRTDEGQVLEGLREDMLETLVPKVPGDQVMVVLGQWAGR- CALJAX_48_PE57 NOISO GQYYNTKMIIEDVLSPDTCVCQTDEGRVLEGLREDMLETLVPKAEGDRVMVVLGPQAGR- CANFAX_43_PE41 NOISO GQYYNTKMTIEDVLSPDTCVCRTDEGRILEGLKENMLETLVPKVEGNRVMVVLGPQAGR- CAVPO132_3_PE19 NOISO GQYYNTKMTIEDVLSPDTCVCRTDKGQVLEGLREDMLETLVPKVEGDRVMVVLGPQAGR- DANRE8_40_PE13 ENSDARG00000029612 GKYYNSKMRVEDVLTPHTCVCRTEEGRMLDDIRQKMLETIVPKNDSDYIMVVLGEHRGQ- DANRE8_40_PE14 ISOFORMIN ------------------------------------------------------------ DASNOGS_505_PE1 NOISO GQYYNTKMTIEDVLTPDTCVCRTDEGRVLEGLRENMLETLIPKAEGDCVMVVLGPQAGR- DIPORGS_488_PE1 NOISO GQYYNTKMIIEDVLSPDTCVCRTDNGQILEGLKENMLETLVPKEEGDLVMVVLGTQVGK- ERIEUGS_624_PE1 NOISO XXXXXXXMTIEDVLSPSTCVCKTDEGRILEXXXXXXLETLIPKVEGNRVMVVLGPKAGR- FELCAGS_330_PE1 NOISO -----DLMTIEDVLSPDTCECLTDEGLVLEGLREDMLETLVSKVQGN----VLGPQAGR- FUGRU226_PE32 ENSTRUG00000000616 GKYYNSKMFVEDVLTPTSCVCRTQEGRLVDDVKQEMLETIVPKGDNESIMVVLGEQRGQ- FUGRU226_PE33 ISOFORMIN GKYYNSKMFVEDVLTPTSCVCRTQEGRLVDDVKQEMLETIVPKGDNESIMVVLGEQRGQ- FUGRU226_PE34 ISOFORMIN GKYYNSKMFVEDVLTPTSCVCRTQEGRLVDDVKQEMLETIVPKGDNESIMVVLGEQRGQ- GASACGROUPXII_17_PE10 ENSGACG00000012127 GKYYNSKMRVEDVLSPTMCVCRTEEGRLLDDVKQNMLETIIPKSEDDPIMVVLGEHRGQ- GASACGROUPXII_17_PE11 ISOFORMIN ------------------------------------------------------------ GORGOX_52_PE26 NOISO GQYYNTKMIIEDVLSPDTCVCRTDEGRVLEGLREDMLETLVPKAEGDRVMVVLGPQTGR- HORSEX_41_PE12 NOISO GQYYNTKMTIEDVLSPSTCVCRTDEGQVLEGLREDMLETLVPKVEGNRVMVVLGPKAGR- HSX_PE902 ENSG00000068394 GQYYNTKMIIEDVLSPDTCVCRTDEGRVLEGLREDMLETLVPKAEGDRVMVVLGPQTGR- HSX_PE903 ISOFORMIN ------------TGCTGTCVCGLWTTCTK----EANIT--TPR----------------- LOXAF56_2_PE8 NOISO GQYYNIKMTVEDVLSPDTCVCRTDEGQILEGLREDMLETLIPKVEGNRVMVVLGPQAGR- MACEUGS_741_PE1 NOISO GRYYNTKMIIEDVLSPDTCVCRTDEG---------------------------------- MACMUX_48_PE33 NOISO GQYYNTKMIIEDVLSPDTCVCRTDEGRVLEGLREDMLETLVPKAEGDRVMVVLGPQAGR- MICMU15422_PE1 NOISO GQYYNTKMTIEDVLSPDTCVCRTDEGRVLEGLKEDMLETLVPKAEGDCVMVVLGPHAGR- MONDO4_393_PE11 NOISO GRYYNTKMIIEDVLSPDTCVCRTDEGRLLEGLREDMLETLIPKEEKATVMVVLGPQRGQ- MONDOUN_64_PE2 NOISO GRYYNTKMIIEDVLSPDTCVCRTDEGRLLEGLREDMLETLIPKEEKATVMVVLGPQRGQ- MOUSEX_PE58 ENSMUSG00000031148 GRYYNTKMTIEDVLSPDTCVCRTDEGRVLEDVREDMLETLIPKGEGHRVMVVLGPHAGK- MOUSEX_PE59 ISOFORMIN GRYYNTKMTIEDVLSPDTCVCRTDEGRVLEDVREDMLETLIPKGEGHRVMVVLGPHAGK- NEMVE_9979_PE55 NOISO GRYYNTKVKVVDVISRNECDCETEDGKLLEGVKQSMLETVVPKKQDAYIRIVRGPSKGQ- OCHPRGS_331_PE1 NOISO GQYYNTKMTIEDLLSPDTCVCRTDEGRVLEGVKENMLETLIPKAEGERVMVVLGPQAGR- ORNANULTRA295_PE13 NOISO GRYYNCKMIIEDVVSPDTCVCRTEDGRVLEGVKEAMLETLIPKDEDNWIMVVLGQFSGR- ORYLA7_22_PE39 NOISO GRFYNSKMQVEDVLTPFTCVCRTEEGRLLDDVKQDMLETIIPKSDGDSVMVVLGEHRGQ- OTOGAGS_386_PE1 NOISO GQYYNTKMTIEDVLSPDTCVCRTDEGLVLEGLREDMLETLVPKAEGDRVMVVLGSHAGR- PIGX_44_PE8 NOISO GQYYNTKMTIEDVLSPDTCVCRTDEGQVLEGLREDMLETLVPKVEGHRVMVVLGPRAGR- PONPYX_50_PE28 NOISO -------------------------------LREDMLETLVPKAEGDRVMVVLGPQAGR- RABITX_35_PE16 NOISO GQYYNTKMTIEDLLSPDTCVCRTDEGRVLEGVKEDMLETLVPKTEGERVMVVLGPQAGR- RATX_PE254 NOISO GRYYNTKMTIEDVLSPGTCVCRTDEGRVLEGVKEGMLETLVPRGEGHRVMVVLGPHAGK- SORARGS_498_PE1 NOISO GRYYNTKMTIEDVLSPGICVCRTDEGRILEGLKEDMLETLVPKVEGHRVMVVLGPQAGK- SPETRGS_404_PE1 NOISO GQYYNTKMT-EDVLSPDIXXXXXXXXXXXXXLREDMLETLVPKVEGNRVMVVLGPQAGR- TETNG9_PE628 ENSTNIG00000012502 GRYYNSKMLVEDVLTPTSCVCRTEEGRIVEDVQQDMLETVVPKSDSETIMVVLGEQRGQ- TETNG9_PE629 ISOFORMIN GRYYNSKMLVEDVLTPTSCVCRTEEGRIVEDVQQDMLETVVPKSDSETIMVVLGEQRGQ- TRIAD_1217_PE476 NOISO GRYYNHKVKIIDVIRAGTCICETDDGRLLENITEDMLETIVPKGNPAYVIVVKGKHAGM- TUPGBGS_385_PE1 NOISO GRYYNSKMTIEDVLSPDTCVCRTDEGQILEGLREDMLETLVPKAEGTRVMVVLGPQAGR- TURTR89660_PE1 NOISO GQYYNTKMTVEDVLSPDTCVCRTDEGQVLEGLREDMLETLVPKVQGNRVMVVLGPWAGR- XENTR154_2_PE15 NOISO ------------------------------------------------------------ MATCH CONSENSUS G?YYN?KM??EDVL?P??CVC?T??G?????????MLET??PK??????MVVLG???G??

AILMEGL192563_1_1_PE14 NOISO VGRLLGRDRERSRALVQLRR--ENQLVELHYDAVCQYLGPSDSDED ANOCA553_PE5 NOISO VGRILRRDKGQNRALVQLQQ-DEEKLLTLDYDAVCLYVGGSEED-- BOVINX_57_PE10 NOISO VGRLLDRDRARSRALVQLQR--EDRVVELHYDAICQFVGLWDVEED CALJAX_48_PE57 NOISO VGHLLSRDRARSRALVQLPR--ENQVVELHYDAVCQYTGPSDTDED CANFAX_43_PE41 NOISO VGRLLGRNRERSRALVQLRR--ENQLVELHYDAVCQYMGPSDSDED CAVPO132_3_PE19 NOISO VGHLLGRDRAHNRALVQLQR--ENQLVELHYDAVCQFMGPIDFDED DANRE8_40_PE13 ENSDARG00000029612 VGRILKRDREKCRAMVQLDR-YEERVFTLDYDTICHYVGGTDH--- DANRE8_40_PE14 ISOFORMIN ---------------------------------------------- DASNOGS_505_PE1 NOISO VGRLLDRDRERSRALVQLRR--NNQLVELHYDAVCQYMGPSDSDEN DIPORGS_488_PE1 NOISO LGHLLDRDRARNRALVQLQS--ENEAVELHYDAICQYMGPSNLDED ERIEUGS_624_PE1 NOISO VGRLLDQDRERSRALVQLRR--ENQVLELHYDAVCQYVGPSDSEED FELCAGS_330_PE1 NOISO VGRLLDWDRERSRALVQLWR--ENQFVELHYDAVCQYLGPSNSDED FUGRU226_PE32 ENSTRUG00000000616 VGRILQRDKNKCRALVQLDR-YEEKVFPLDYDTICHYVGGADR--- FUGRU226_PE33 ISOFORMIN VGRILQRDKNKCRALVQLDR-YEEKVFPLDYDTICHYVGGAD---- FUGRU226_PE34 ISOFORMIN VGRILQRDKNKCRALVQLDR-YEEKVFPLDYDTICHYVGGAD---- GASACGROUPXII_17_PE10 ENSGACG00000012127 VGRILQRDKNKCRATVQLDR-SEEKLFTLDYDTICHYVGATDR--- GASACGROUPXII_17_PE11 ISOFORMIN ---------------------------------------------- GORGOX_52_PE26 NOISO VGHLLSRDRARSRALVQLPR--ENQVVELHYDAICQYMGPSDTDDD HORSEX_41_PE12 NOISO VGRLLGRDGERSRALVQLRR--ENRLVELHYDAVCQYVGPSDSEED HSX_PE902 ENSG00000068394 VGHLLSRDRARSRALVQLPR--ENQVVELHYDAICQYMGPSDTDDD HSX_PE903 ISOFORMIN ---------------------------------------------- LOXAF56_2_PE8 NOISO VGRLLGWDTEKSQALVQLQR--ENQLVELHYDAVCQYMGPSDSDED MACEUGS_741_PE1 NOISO ---------------------------------------------- MACMUX_48_PE33 NOISO VGHLLSRDRARSRALVQLPR--EDQVVELHYDAICQYMGPSDTDDD MICMU15422_PE1 NOISO VGHLLGRDIARSRALVQLRR--ENQVVELHYDAVCQYVGPSDTDED MONDO4_393_PE11 NOISO VGRILARDKERSQALVQLLQEGEGTVLRLDFDAVCQYVGPVAED-- MONDOUN_64_PE2 NOISO VGRILARDKERSQALVQLLQEGEGTVLRLDFDAVCQYVGPVAED-- MOUSEX_PE58 ENSMUSG00000031148 VGLLRSRDRAQSHALVQLRR--ENQVVELHYNAICQYMGPGDSDED MOUSEX_PE59 ISOFORMIN VGLLRSRDRAQSHALVQLRR--ENQVVELHYNAICQYMGPGDSDED NEMVE_9979_PE55 NOISO LATIMQRDTSQSEAIIQLLL--DKSIETYPYDDICEHLGSIREDLM OCHPRGS_331_PE1 NOISO VGQLLRRSTTHSRALVRLRR--DTQLLELHYDAVCQYMGPSDSDED ORNANULTRA295_PE13 NOISO LGRILRRDRERSRALVQLQRDEEGPVLKLDYDAICHYVGHVDED-- ORYLA7_22_PE39 NOISO VGRILQRDKNKCKATVQLNR-YEEKLFTLDYDFICHYVGAEDQ--- OTOGAGS_386_PE1 NOISO VGHLLGRDRARSQALVQLQR--ENQVVELHYDAVCQYVGPSDTDED PIGX_44_PE8 NOISO GGRLLKRDRERSRALVQLRK--ENQVVELHYDAICQYVGPSDSDED PONPYX_50_PE28 NOISO VGHLLSRDRARSRALVQLPR--ENQVVELHYDAICQYMGPSDTDDD RABITX_35_PE16 NOISO VGQLLRRDTARSRALVQLHR--DDQLVELHYDAVCQYMGPSDSDED RATX_PE254 NOISO VGLLLRRDRAQSHAMVQLGR--ENQVVELHYDAICQYMGPGDSDED SORARGS_498_PE1 NOISO VGRLLGRDQARSRAMVQLQR--ENQLLELHYDAVCQYMGPSDAEDD SPETRGS_404_PE1 NOISO VGHLLSRDRAKSRALVQLRR--DKQLVELHYDAVCQYMGPSDSDED TETNG9_PE628 ENSTNIG00000012502 VGRILQRDKNKCRAMVQLDR-YEEKVFTLDYDTICHYVGGA----- TETNG9_PE629 ISOFORMIN VGRILQRDKNKCRAMVQLDR-YEEKVFTLDYDTICHYVGGA----- TRIAD_1217_PE476 NOISO VGKIVSRDKSNYKAVAYLLS--NQKAAEFDYDDICEYTGDIDHEDY TUPGBGS_385_PE1 NOISO VGHLLRRDRARSQALVQLQR--ENQLVELHYDAICQYVGPSDADED TURTR89660_PE1 NOISO VGRLLDRDREQSRALVQLQR--ENQVVELHYDAICQYMGPSDSED- XENTR154_2_PE15 NOISO ---------------------------------------------- MATCH CONSENSUS VG??L?RD?????A?VQL??????????L?YD??C?Y?G???????


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