Alignment of the gene Family HOG000293426 of HOGENOM

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Threshold: 50% 80% 100%
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Isoforms are ON
AILMEGL192808_1_1_PE1 ENSAMEG00000001973 ------------------------------------------------------------ AILMEGL192808_1_1_PE2 ISOFORMIN -PG--------------LGTSANLPGPASRPAEARTVKSL----NLNKQTTAIPDDSSQA ANOCA79_5_PE1 NOISO ------------------------------------------------------------ BOVIN19_30_PE7 ENSBTAG00000000120 DPS--------------AGAAAEEEPPAPRPRRRRSLRRLFGRFLLALGSRSRLGDSPPR BOVIN19_30_PE8 ISOFORMIN DPS--------------AGAAAEEEPPAPRPRRRRSLRRLFGRFLLALGSRSRLGDSPPR BOVIN19_30_PE9 ISOFORMIN ------------------------------------------------------------ CALJA5_74_PE21 ISOFORMIN GLRV-------------WSCAGGSHGPGPRGRGRRSLRRLFSRFLLALGSRSRPGDLPPW CALJA5_74_PE22 ENSCJAG00000012679 D-PG-------------PEDAAGGGPPAPRPRRRRSLRRLFSRFLLALGSRSRPGDLPPW CANFA5_38_PE5 NOISO ------------------------------------------------------------ CHOHOGS_5925_PE2 NOISO -------------------------------------------------SRSRPGDAPPP CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 ------------------------------------------------------------ DANRE3_27_PE27 NOISO D------KRDKKANKDSGSKSTKRTGKLFRRKSFKSVESLVSRILKSLGSLASGGETAE- DANRE6_20_PE8 ENSDARG00000060632 DSLRETRKQTIKAKSAKRKNKTHKSQKLFRRKSLKSLGNFMSRIVKTLGTLAHLGDADQP DANRE6_20_PE9 ISOFORMIN ------------------------------------------------------------ DASNOGS_5350_PE2 NOISO DSD--------------PGPAAGEGPPAPRPRRRRSLRRLLGRFLLALGSRSRPGASPPQ DIPORGS_4986_PE2 NOISO DPG--------------PGDPAGERPAAPRPRRRRSLRRLFSRFLLALGSRSRPRDSPPR ERIEUGS_5458_PE1 NOISO ------------------------------------------------------------ FELCAGS_3581_PE2 NOISO ------------------------------------------------------------ FUGRU26_1_PE135 NOISO ------------------------------------------------------------ FUGRU65_1_PE42 NOISO ------------------------------------------------------------ GASACGROUPIX_18_PE15 NOISO ------------------------------------------------------------ GASACGROUPXI_4_PE54 ISOFORMIN ------------------------------------------------------------ GASACGROUPXI_4_PE55 ENSGACG00000006879 ------------------------------------------------------------ GORGO17_11_PE4 NOISO -------------------------------RPRRSLRRLFSRFLLALGSRSRPGDSPPR HORSE11_55_PE3 NOISO ---G-------------PGGAAGEGLPAPGPRRRRSLRRLFSRFLLALGSRSRPGDSPPR HS17_PE534 ENSG00000154914 DLGP--------------GDAAGGGPLAPRPRRRRSLRRLFSRFLLALGSRSRPGDSPPR LOXAF47_14_PE7 ENSLAFG00000014238 --AG-------------PGDASAQGP--PLPRRRSSLRRLLSRFLLALGSRARPGASPLR LOXAF47_14_PE8 ISOFORMIN ----------------------------PLPRRRSSLRRLLSRFLLALGSRARPGASPLR MACEUGS_6207_PE1 NOISO DPG--------------RGGAAGEGPPAPL-RRRRSFRRLLGRFLRVLGVLSRSEDSPPR MACMU16_10_PE4 ENSMMUG00000014039 DPGP--------------GDAAGGGPPAPRPRRRRSLRRLFSRFLLALGSRSRPGDSPPR MICMUGS_287_PE4 NOISO ------------------------------------------------------------ MONDO2_262_PE1 NOISO ------------------------GGPSAPPRRRRSFRRLLGRFLRVLGVLPSSGVSSPE MOUSE11_PE1552 ENSMUSG00000020905 DPG--------------VGNALGEGPPAPRPRRRRSLRRLLNRFLLALGSRSRSGDSPPR MOUSE11_PE1553 ISOFORMIN DPG--------------VGNALGEGPPAPRPRRRRSLRRLLNRFLLALGSRSRSGDSPPR MOUSE11_PE1554 ISOFORMIN ------------------------------------------------------------ OCHPRGS_5271_PE1 NOISO DPGP-------------GDAVAEGPPAAPRPRRRRSLRRLFSRFLLALGSRSRSGDSPPR ORYLA1_12_PE4 NOISO ------------------------------------------------------------ PANTR17_49_PE8 NOISO DLGP--------------GDAAGGGLLAPRPRRRRSLRRLFSRFLLALGSRSRPGDSPPR PIG12_54_PE2 NOISO DPG--------------AGAAAAEGPPAPRPRRRRSLRRLFTRFLLALGSRSRPGDSPSR PONPY17_11_PE3 NOISO DLGP--------------GDAAGGGLLAPRPRRRRSLRRLFSRFLLALGSRSRPGDSPPR PROCAGS_5529_PE2 NOISO ------------------------------------------------------------ PTEVAGS_2827_PE2 NOISO EPG--------------SEAAAGEGRPAPRPRRRRSLRRLLSRFLLALGSRSRPEDSPPR RABIT19_11_PE2 NOISO ------------------------------------------------------------ RAT10_PE1005 ENSRNOG00000003785 --------------------------------RRRSLRRLLNRFLLALVTRSRSGDSPPR RAT10_PE1006 ISOFORMIN ------------------------------------------------------------ RAT10_PE1007 ISOFORMIN --------------------------------RRRSLRRLLNRFLLALVTRSRSGDSPPR RAT10_PE1008 ISOFORMIN --------------------------------RRRSLRRLLNRFLLALVTRSRSGDSPPR SORARGS_4441_PE1 NOISO ------------------------------------------------------------ TARSYGS_5169_PE1 NOISO ------------------------------------------------------------ TETNGUNRD_PE1034 NOISO ------------------------------------------------------------ TETNGUNRD_PE266 NOISO ------------------------------------------------------------ TURTRGS_195_1_PE4 NOISO DPG--------------AGAATGEGPPAPRPRRRRSLRRLFGRFLLALGSRSRPGDSPPR XENTR255_2_PE8 NOISO ------------------------------------------------------------ MATCH CONSENSUS ????????????????????????????????????????????????????????????

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 ------------------------------------------------------------ AILMEGL192808_1_1_PE2 ISOFORMIN NARLPVLESLLDGADGL--EGGDATCPHTPAPAG-GTPA-------PPP----RRSPLPV ANOCA79_5_PE1 NOISO ------------------------------------------------------------ BOVIN19_30_PE7 ENSBTAG00000000120 PR----PQPQSGHGDGD-GEGGFE---GK-DPAAPGSPGE-----ERPP-GPQPQL---- BOVIN19_30_PE8 ISOFORMIN PR----PQPQSGHGDGD-GEGGFE---GK-DPAAPGSPGE-----ERPP-GPQPQL---- BOVIN19_30_PE9 ISOFORMIN ------------------------------------------------------------ CALJA5_74_PE21 ISOFORMIN P--------QPGPCDGD-GEGGFACAAGP-APEAPGSPGE-----ERPP-GPHPQLQLPA CALJA5_74_PE22 ENSCJAG00000012679 P--------QPGPCDGD-GEGGFACAAGP-APEAPGSPGE-----ERPP-GPHPQLQLPA CANFA5_38_PE5 NOISO ------------------------------------------------------------ CHOHOGS_5925_PE2 NOISO P--------QPGHGDGD-GEGGFASAPGS-TPGAPGSPEE-----ERPP-RPQPQL---- CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 ------------------------------------------------------------ DANRE3_27_PE27 NOISO R------------SDAEDDDGGFGESAPCAGNSVKKEDSSTSSSQVRKSSSLTSRSSAKE DANRE6_20_PE8 ENSDARG00000060632 D------------AAAEDDDGGFGDTASVHNSGNYRESSA------RK---SSSYGSVKE DANRE6_20_PE9 ISOFORMIN ------------------------------------------------------------ DASNOGS_5350_PE2 NOISO A--------QPGRGDGD-GDGGFAGAPGP-APGAPGSPGE-----ERAP-SPQPQL---- DIPORGS_4986_PE2 NOISO SQ----PQPQPGHCDGD-GEGGFACAPSP-ALAAPGSPVA-----ERLL-GPPSQL---- ERIEUGS_5458_PE1 NOISO ----------PGRCDGD-GEGGFACPAGP-GPTAPGSPGE-----ERAA-RPQPRP---- FELCAGS_3581_PE2 NOISO ------------------------------------------------------------ FUGRU26_1_PE135 NOISO ------------------------------------------------------------ FUGRU65_1_PE42 NOISO ------------------------------------------------------------ GASACGROUPIX_18_PE15 NOISO ------------------------------------------------------------ GASACGROUPXI_4_PE54 ISOFORMIN ------------------------------------------------------------ GASACGROUPXI_4_PE55 ENSGACG00000006879 ------------------------------------------------------------ GORGO17_11_PE4 NOISO P--------QPGHCDGD-GEGGFACAPGP-VPAAPGSPGE-----ERPP-GPQPQ--LPA HORSE11_55_PE3 NOISO PQ----PQPQPGHCDGD-GEGGFTCAPGP-APAAPGSPGE-----ERPP-RSQTQL---- HS17_PE534 ENSG00000154914 P--------QPGHCDGD-GEGGFACAPGP-VPAAPGSPGE-----ERPP-GPQPQLQLPA LOXAF47_14_PE7 ENSLAFG00000014238 ----------PGSCNGE-GEGGFAHARVP-APSAPGSPGE-----ERQP-RPQPQL---- LOXAF47_14_PE8 ISOFORMIN ----------PGSCNGE-GEGGFAHARVP-APSAPGSPGE-----ERQP-RPQPQL---- MACEUGS_6207_PE1 NOISO PEP--------GSGGGD-GS---GHSPWP-AGEVPWEAGS-----AR-----------PA MACMU16_10_PE4 ENSMMUG00000014039 P--------QPGYCDGD-GEGGFACAPGP-APAAPGSPGE-----ERPP-GPQPQLQLPA MICMUGS_287_PE4 NOISO ------------------------------------------------------------ MONDO2_262_PE1 NOISO TQPGVN---QSGTGRGSNSSEGFGDSARS-ANEVSREAGV-----ARLP-Q--------- MOUSE11_PE1552 ENSMUSG00000020905 PP----AQ--PSPYDGD-GEGGFACAPGP-APASAGSPGP-----DRPP-GSQPQI---- MOUSE11_PE1553 ISOFORMIN PP----AQ--PSPYDGD-GEGGFACAPGP-APASAGSPGP-----DRPP-GSQPQI---- MOUSE11_PE1554 ISOFORMIN ------------------------------------------------------------ OCHPRGS_5271_PE1 NOISO P--------QPGYCDGD-GEGGFACAPGAPASGAPGSPGE-----EHPP-GSQPQF---- ORYLA1_12_PE4 NOISO ------------------------------------------------------------ PANTR17_49_PE8 NOISO P--------QPGHCDGD-GEGGFACAPGP-VPAAPGSPGE-----ERPP-GPQPQLQLPA PIG12_54_PE2 NOISO PR----SQPPPGHGDGD-GEGGFASAPGA-DPAAPGSPGE-----ERPP-RPQPQL---- PONPY17_11_PE3 NOISO P--------QPGHCDGD-GEGGFACAPGP-VPAAPGSPGE-----ERPP-GPQPQLQLPA PROCAGS_5529_PE2 NOISO ------------------------------------------------------------ PTEVAGS_2827_PE2 NOISO P--------QPGHCDGD-GEGGFACAPAP-APDAPGSPGE-----ERQP-GPQSQL---- RABIT19_11_PE2 NOISO ------------------------------------------------------------ RAT10_PE1005 ENSRNOG00000003785 AP----AQ--PSPYDGD-GEGGFACAPGP-APASSGSPGP-----DRPL-GSQPQI---- RAT10_PE1006 ISOFORMIN -------------------------------------------------------M---- RAT10_PE1007 ISOFORMIN AP----AQ--PSPYDGD-GEGGFACAPGP-APASSGSPGP-----DRPL-GSQPQI---- RAT10_PE1008 ISOFORMIN AP----AQ--PSPYDGD-GEGGFACAPGP-APASSGSPGP-----DRPL-GSQPQI---- SORARGS_4441_PE1 NOISO ------------------------------------------------------------ TARSYGS_5169_PE1 NOISO ------------------------------------------------------------ TETNGUNRD_PE1034 NOISO ------------------------------------------------------------ TETNGUNRD_PE266 NOISO ------------------------------------------------------------ TURTRGS_195_1_PE4 NOISO PQ------PQPGHGNGD-GEGGFAGPPGP-DPAAPGSPGE-----ERPP-RPQPQL---- XENTR255_2_PE8 NOISO ------------------------------------------------------------ MATCH CONSENSUS ????????????????????????????????????????????????????????????

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 -----------GAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- AILMEGL192808_1_1_PE2 ISOFORMIN RVSRCARGCPLGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- ANOCA79_5_PE1 NOISO ------------------------------------------------------------ BOVIN19_30_PE7 ENSBTAG00000000120 --PAGDAARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALRRYRA----------- BOVIN19_30_PE8 ISOFORMIN --PAGDAARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALRRYRA----------- BOVIN19_30_PE9 ISOFORMIN ----GDAARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALRRYRA----------- CALJA5_74_PE21 ISOFORMIN GSPAGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGH-------------- CALJA5_74_PE22 ENSCJAG00000012679 ----GDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGHYRA----------- CANFA5_38_PE5 NOISO ------------------------------------------------------------ CHOHOGS_5925_PE2 NOISO --PGGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 GFDLSSINSMPGVVGLTNH-NTCFMNSIVQCLSNTDVLAEYFVLGQYKNDLKNCRKDR-- DANRE3_27_PE27 NOISO RMFSLYGDKTPGVLGLKNHGNTCFMNAVVQCLSNTDLLAEYLGLERYKRDVCHNGINGFI DANRE6_20_PE8 ENSDARG00000060632 RLSWCYGEKTPGVQGLKNHGNTCFMNAVVQCLSNTELLAEYLGLEQYKYELNERRINGMM DANRE6_20_PE9 ISOFORMIN -----------GVQGLKNHGNTCFMNAVVQCLSNTELLAEYLGLEQYKYELNERRINGMM DASNOGS_5350_PE2 NOISO --PAGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYQA----------- DIPORGS_4986_PE2 NOISO --PAGNGVRPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- ERIEUGS_5458_PE1 NOISO --LAGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- FELCAGS_3581_PE2 NOISO ------------------------------------------------------------ FUGRU26_1_PE135 NOISO ------GDKTPGVLGLKNHGNTCFMNAVVQCLSNTDLLAEYLGLEQYRP----------- FUGRU65_1_PE42 NOISO ------GDQVPGVMGLKNHGNTCFMNAVVQCLSNTNVLAEYLGLKRYKHDLVQTGLNGVL GASACGROUPIX_18_PE15 NOISO ------GDKTPGVLGLKNHGNTCFMNAVVQCLSNTDLLAEHLGLEQYKS----------- GASACGROUPXI_4_PE54 ISOFORMIN ----QFADKIPGVVGLKNHGNTCFMNAVVQCLNNTDLLAEYLGLERYKLDLGHAAVRAHG GASACGROUPXI_4_PE55 ENSGACG00000006879 ------------------------MNAVVQCLNNTDLLAEYLGLERYKLDLGHAAVRAHG GORGO17_11_PE4 NOISO ----GDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- HORSE11_55_PE3 NOISO --PAGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- HS17_PE534 ENSG00000154914 ----GDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- LOXAF47_14_PE7 ENSLAFG00000014238 --PAGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- LOXAF47_14_PE8 ISOFORMIN --PAGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- MACEUGS_6207_PE1 NOISO QPGPGDKARPPGTQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGHYRA----------- MACMU16_10_PE4 ENSMMUG00000014039 ----GDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- MICMUGS_287_PE4 NOISO ------------------------------------------------------------ MONDO2_262_PE1 NOISO -PPPGDKARPPGTQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGHYQA----------- MOUSE11_PE1552 ENSMUSG00000020905 --SSGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- MOUSE11_PE1553 ISOFORMIN --SSGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- MOUSE11_PE1554 ISOFORMIN ------------------------MNAVVQCLSNTDLLAEFLALGRYRA----------- OCHPRGS_5271_PE1 NOISO --AGDDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- ORYLA1_12_PE4 NOISO ------GDKTPGVLGLKNHGNTCFMNAVVQCLSNTDLLAEYLGLEQYKS----------- PANTR17_49_PE8 NOISO ----GDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYQA----------- PIG12_54_PE2 NOISO --PAGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- PONPY17_11_PE3 NOISO ----GDGARPPGA--------------------NTDLLAEFLALGRYRA----------- PROCAGS_5529_PE2 NOISO ------------------------------------------------------------ PTEVAGS_2827_PE2 NOISO --SASDGARPPGTQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- RABIT19_11_PE2 NOISO ------------------------------------------------------------ RAT10_PE1005 ENSRNOG00000003785 --SSGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- RAT10_PE1006 ISOFORMIN --AMEKG-------------A--LL---APRARHQPLLAAQGQIVLWDL----------- RAT10_PE1007 ISOFORMIN --SSGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- RAT10_PE1008 ISOFORMIN --SSGDGARPPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- SORARGS_4441_PE1 NOISO ------------------------------------------------------------ TARSYGS_5169_PE1 NOISO ------------------------------------------------------------ TETNGUNRD_PE1034 NOISO ------GDKTPGVLGLKNHGNTCFMNAVVQCLSNTDLLAEYLGLEQYRDR-----VNGEL TETNGUNRD_PE266 NOISO ------------------------------------------------------------ TURTRGS_195_1_PE4 NOISO --SAGDGARIPGAQGLKNHGNTCFMNAVVQCLSNTDLLAEFLALGRYRA----------- XENTR255_2_PE8 NOISO ------------------------MNAVVQCLSNTDMLAEFLGMERYKAQ---------- MATCH CONSENSUS ?????????????????????????????????NT??LAE????????????????????

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 -----APXXXXXXX---XXXXXXXTREYTPQLSAEFK---NAVSKYGSQFQGNAQHDALE AILMEGL192808_1_1_PE2 ISOFORMIN -----APX----------------TREYTPQLSAEFK---NAVSKYGSQFQGNAQHDALE ANOCA79_5_PE1 NOISO ---------------------------------------QNVVSKYGAQFRGNSQHDALE BOVIN19_30_PE7 ENSBTAG00000000120 -----EPGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGAQFQGNAQHDALE BOVIN19_30_PE8 ISOFORMIN -----EPGRAEVTEQLAALVRALWTREYTPQLSAEFKFLQNAVSKYGAQFQGNAQHDALE BOVIN19_30_PE9 ISOFORMIN -----EPGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGAQFQGNAQHDALE CALJA5_74_PE21 ISOFORMIN ----------EVTEQLAALVRALWTREYTPQLSAEFK--QNAVSKYGSQFQGNSQHDALE CALJA5_74_PE22 ENSCJAG00000012679 -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE CANFA5_38_PE5 NOISO ------------------------------------------------------------ CHOHOGS_5925_PE2 NOISO -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK-FQNAVSKYGSQFQGNAQHDALE CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 --SKKYGTKGEVIELLASVVRSLWTVTYSQDMTKSLK---DSISKYGAQYKGSNQHDSQE DANRE3_27_PE27 NOISO RNGNGQNEKGEVTERLAVLVRALWTFQYTPQLSAEF---KMTVSKYGAQFRGNSQHDALE DANRE6_20_PE8 ENSDARG00000060632 KSE---EVRGEVTDKLASLVRALWTNDYTPQLSVDF---KSVVAKYGSQFKGNAQHDALE DANRE6_20_PE9 ISOFORMIN KSE---EVRGEVTDKLASLVRALWTNDYTPQLSVDF---KSVVAKYGSQFKGNAQHDALE DASNOGS_5350_PE2 NOISO -----APGRAEVTEQLAALVRALWTRDYTPQLSAEFKXXXXXXXXXXXXXXXXXXXXXX- DIPORGS_4986_PE2 NOISO -----APGRAEVTEQLAALVRALWTREYTPQLSAEFKXXXXXXXXXXXXXXXXXXXXXXX ERIEUGS_5458_PE1 NOISO -----APGRAKVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE FELCAGS_3581_PE2 NOISO --------------------------------------CQNAVSKYGSQFQGNAQHDALE FUGRU26_1_PE135 NOISO -----DAARGEVTEHLASLVRALWTLEYTPQLSVEFK-FRSTVAKYASQFRGNSQHDALE FUGRU65_1_PE42 NOISO ISDGTRHVKGEVTEQLATLVRALWTLEYTPQLSVDFK-FKLIVSKYGSQFRGNSQHDALE GASACGROUPIX_18_PE15 NOISO -----EAARGEVTEQLASLVRGLWTLEYTPQVSVEFK--QSIVAKYGSQFRGNSQHDALE GASACGROUPXI_4_PE54 ISOFORMIN RSAETRPAGGEVTEQLASLVRALWTLEYTPQLSVDF---KTVVSKYGSQFRGNSQHDALE GASACGROUPXI_4_PE55 ENSGACG00000006879 RSAETRPAGGEVTEQLASLVRALWTLEYTPQLSVDF---KTVVSKYGSQFRGNSQHDALE GORGO17_11_PE4 NOISO -----APGRAEVTEQLAALVRALWTREYTPQLSAEF---KNAVSKYGSQFQGNSQHDALE HORSE11_55_PE3 NOISO -----APGRAEVTEQLAALVRALWTREYTPQLSAEF---KNAVSKYGSQFQGNSQHDALE HS17_PE534 ENSG00000154914 -----APGRAEVTEQLAALVRALWTREYTPQLSAEF---KNAVSKYGSQFQGNSQHDALE LOXAF47_14_PE7 ENSLAFG00000014238 -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE LOXAF47_14_PE8 ISOFORMIN -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE MACEUGS_6207_PE1 NOISO -----RPGRAEVTEQLAGLVRALWTRDYTPDLSVEFKXXXXXXXXXXXXXXXXXXXXXXX MACMU16_10_PE4 ENSMMUG00000014039 -----APGRAEVTEQLAALVRALWTREYTPQLSAEF---KNAVSKYGSQFQGNSQHDALE MICMUGS_287_PE4 NOISO ------------------------------------------------------------ MONDO2_262_PE1 NOISO -----WPGRAEVTEQLAGLVRSLWTQDYTPHLSVEFKVG-----RTGQGLAGPYP-FSGV MOUSE11_PE1552 ENSMUSG00000020905 -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE MOUSE11_PE1553 ISOFORMIN -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE MOUSE11_PE1554 ISOFORMIN -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE OCHPRGS_5271_PE1 NOISO -----APGSAEVTEQLAALVRALWTREYTPQLSAEFK-FQNAVSKYGSQFQGNSQHDALE ORYLA1_12_PE4 NOISO -----DICQGEVTEQLAALIRALWTLEYTPQLSVDFK--KSIVAKYSSQFRGNSQHDALE PANTR17_49_PE8 NOISO -----APGRAEVTEQLAALVRALWTREYTPQLSAEFKF-QNAVSKYGSQFQGNSQHDALE PIG12_54_PE2 NOISO -----VPGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE PONPY17_11_PE3 NOISO -----APGRAEVTEQLAALVRALWTREYTPQLSAEFKF-QNAVSKYGSQFQGNSQHDALE PROCAGS_5529_PE2 NOISO -----------------------------PQLSAEFK-SQNAVSKYGSQFQGNSQHDALE PTEVAGS_2827_PE2 NOISO -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK-FQNAVSKYGSQFRGNSQHDALE RABIT19_11_PE2 NOISO -------------------------RQRAPR--RPLSSFQNAVSKYGSQFQGNSQHDALE RAT10_PE1005 ENSRNOG00000003785 -----VPGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE RAT10_PE1006 ISOFORMIN -----NPRYLRVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE RAT10_PE1007 ISOFORMIN -----VPGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE RAT10_PE1008 ISOFORMIN -----VPGRAEVTEQLAALVRALWTREYTPQLSAEFK---NAVSKYGSQFQGNSQHDALE SORARGS_4441_PE1 NOISO ----------------------------------------NAVSKYGSQFQGNYQHDALE TARSYGS_5169_PE1 NOISO --------------------------------------FQNAVSKYGSQFQGNSQHDALE TETNGUNRD_PE1034 NOISO GADERQAARGDVTEHLASLVRALWTLEYTPQLSVEF---KSTVAKYASQFRGNSQHDALE TETNGUNRD_PE266 NOISO ------------------------------------------------------------ TURTRGS_195_1_PE4 NOISO -----APGRAEVTEQLAALVRALWTREYTPQLSAEFK-FQNAVSKYGSQFQGNSQHDALE XENTR255_2_PE8 NOISO -----LSKGGEVTERLASLVRGLWTLEYTPHLSAEFK---NVVSKYGTQFRGNSQHDALE MATCH CONSENSUS ????????????????????????T??YTP?LS??F??????V?KYG?QF?GN?QHDALE

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 FLLWLLDRLHEDLEGSARSPGSEKPQP-----EATRTPE--------NLPSPSAQLSLGQ AILMEGL192808_1_1_PE2 ISOFORMIN FLLWLLDRLHEDLEGSARSPGSEKPQP-----EATRTPE--------NLPSPSAQLSLGQ ANOCA79_5_PE1 NOISO FLLWLLDRMHEDLCSVGRGPRTRSVSA-----KRSQNSN--------RQIFTAAQPSKGQ BOVIN19_30_PE7 ENSBTAG00000000120 FLLWLLDRVHEDLEGSSRGPGSDKLQP-----EARKTSE--------NLLSPSAPLSMGQ BOVIN19_30_PE8 ISOFORMIN FLLWLLDRVHEDLEGSSRGPGSDKLQP-----EARKTSE--------NLLSPSAPLSMGQ BOVIN19_30_PE9 ISOFORMIN FLLWLLDRVHEDLEGSSRGPGSDKINE-----GETEFIL------ALEFREEYSRTECGQ CALJA5_74_PE21 ISOFORMIN FLLWLLDRVHEDLEGSSRGSVSEKLPP-----EANKTSE--------NCLSPSAQLSLGQ CALJA5_74_PE22 ENSCJAG00000012679 FLLWLLDRVHEDLEGSSRGSVSEKLPP-----EANKTSE--------NCLSPSAQLSLGQ CANFA5_38_PE5 NOISO ------------------------------------------------------------ CHOHOGS_5925_PE2 NOISO FLLWLLDRVHEDLDGSARGPVAAKLQP-----EVSRNSE--------NLPSPSPQPSLGR CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 FMLWLFDKMHEDLNMQPVKKRSFTRRPSFRKSVSRKPSENGKGIDDVGAATKLAVDLSPT DANRE3_27_PE27 NOISO FLLWLLDRLHEDSTSVVPNGNNINKSKNKGQEGGDEKPG------SDTLSPPQTQPGVRH DANRE6_20_PE8 ENSDARG00000060632 FLLWLLDCVHEDANLSTNNNQSRNSSSSKGSVGLEESSC------PG--PANSQQPGTRH DANRE6_20_PE9 ISOFORMIN FLLWLLDCVHEDANLSTNNNQSRNSSS--------------------------SKPGTRH DASNOGS_5350_PE2 NOISO XXXXXXXXXXXXXXXXXXXXXXXXLQP-----EVGRNSE--------NLPSPSPQLSLGQ DIPORGS_4986_PE2 NOISO -XXXXXXXXXXXXXXXXXXXXXXXVPP-----EGRRASE--------SLLSSPAQLSLGP ERIEUGS_5458_PE1 NOISO FLLWLLDRVNEDLEGSSRGPEPQK--P-----EVRRTSE--------NLLSPAAQLPLGQ FELCAGS_3581_PE2 NOISO FLLWLLDRVHEDLEVSSRGPGSEKPQP-----EASRTPE--------HLLSPSAQLPLGQ FUGRU26_1_PE135 NOISO FLLWLLDRVHEDVNEISSNAPNSTPQ------KAWISSQ-------IFTSSSTSQPRDRH FUGRU65_1_PE42 NOISO FLLWLLDRVHEDVNPATQNNNTMNVFG-EATKK-----I------GNNVNPASILPLGRH GASACGROUPIX_18_PE15 NOISO FLLWLLDRVHEDANHSPNNNNNSSHKT-----KASVKNL-------V---GVSIWIPISN GASACGROUPXI_4_PE54 ISOFORMIN FLLWLLDRVHEDVNLASHNNNNKTKAPGKGPGGA--EEV------LFPDPSQSEQLRIQH GASACGROUPXI_4_PE55 ENSGACG00000006879 FLLWLLDRVHEDVNLASHNNNNKTKAPGKPPCSQ--QSV------RYN-----PEVGLSH GORGO17_11_PE4 NOISO FLLWLLDRVHEDLEGSSRGPVSEKLPP-----EATKTSE--------NCLSPSAQLPLGQ HORSE11_55_PE3 NOISO FLLWLLDRVHEDLEGSSRGPGSEKLQP-----EASKTSE--------NLLSPPAQLSLGQ HS17_PE534 ENSG00000154914 FLLWLLDRVHEDLEGSSRGPVSEKLPP-----EATKTSE--------NCLSPSAQLPLGQ LOXAF47_14_PE7 ENSLAFG00000014238 FLLWLLDRVHEDLDGSSQRGVSDKLQP-----ETSKHSE---------NLSSTAQLPLGQ LOXAF47_14_PE8 ISOFORMIN FLLWLLDRVHEDLDGSSQRGVSDKLQP-----ETSKHSE---------NLSSTAQLPLGQ MACEUGS_6207_PE1 NOISO XXXXXXXXXXXXXXXXXX--XXXXXXX------XXXXXX---------XXXXXXXXXXXX MACMU16_10_PE4 ENSMMUG00000014039 FLLWLLDRVHEDLEGSSRGPVLEKLPP-----EANKTSE--------NCLSPSAQLPLGE MICMUGS_287_PE4 NOISO ------------------------LPP-----EASKTSE--------NCLSPSAQLSLAQ MONDO2_262_PE1 NOISO FLLWLYQMCHDWTEGSSQGATGSPLSL-----SVSRLS---------GCPRDWLRLFSGQ MOUSE11_PE1552 ENSMUSG00000020905 FLLWLLDRVHEDLEGSAHGLVSEQLPP-----EVSKISE--------DLRPSAAPTSLGP MOUSE11_PE1553 ISOFORMIN FLLWLLDRVHEDLEGSAHGLVSEQLPP-----EVSKISE--------DLRPSAAPTSLGP MOUSE11_PE1554 ISOFORMIN FLLWLLDRVHEDLEGSAHGLVSEQLPP-----EVSKISE--------DLRPSAAPTSLGP OCHPRGS_5271_PE1 NOISO FLLWLLDRMHEDLEGSSRGAASQKLPP-----QASNSE---------NLLASSSQLPSGQ ORYLA1_12_PE4 NOISO FLLWLLDRVHEDANTNPDNEVRALSPP-----QGTSSSE-------DRLNPSTQQPRSRH PANTR17_49_PE8 NOISO FLLWLLDRVHEDLEGSSRGPVSEKLPP-----EATKTSE--------NCLSPSAQLPLGQ PIG12_54_PE2 NOISO FLLWLLDRVHEDLEGSARGPTADKLLP-----EARKTSE--------NPPSPSAQLSLGQ PONPY17_11_PE3 NOISO FLLWLLDRVHEDLEGSSRGPVSEKLPP-----EATKTSE--------NCLSPSAQLPLGQ PROCAGS_5529_PE2 NOISO FLLWLLDRVHEDLDGSSQKGLSEKLQP-----EASKNPE---------NLSPAAQLPLGQ PTEVAGS_2827_PE2 NOISO FLLWLLDRVHEDLEGSTRAPGSEKLQP-----ETSKTSE--------NLLSPSAQLSLGQ RABIT19_11_PE2 NOISO FLLWLLDRVHEDLGGSSRGPASQKLPP-----EASKAPE--------NILSPSARLPPGQ RAT10_PE1005 ENSRNOG00000003785 FLLWLLDRVHEDLEGSTHGQGSEQLPP-----EASKISE--------DLRSSAAPPSLGP RAT10_PE1006 ISOFORMIN FLLWLLDRVHEDLEGSTHGQGSEQLPP-----EASKISE--------DLRSSAAPPSLGP RAT10_PE1007 ISOFORMIN FLLWLLDRVHEDLEGSTHGQGSEQLPP-----EASKISE--------DLRSSAAPPSLGP RAT10_PE1008 ISOFORMIN FLLWLLDRVHEDLEGSTHGQGSEQLPP-----EASKISE--------DLRSSAAPPSLGP SORARGS_4441_PE1 NOISO FLLWLLDRVHKDLEDSPQGLHHK----------ASKTSL--------NSVSPSTQLPLGQ TARSYGS_5169_PE1 NOISO FLLWLLDRVHEDLEGSSRGPVSEKLPP-----EASKTSE--------NRLSPSAELTLGQ TETNGUNRD_PE1034 NOISO FLLWLLDRVHEDEQLKAHKLLQLYPDL-----GEWCNEL-------T---PAALPVLTEP TETNGUNRD_PE266 NOISO ----MMSILHFQYTLASSKPFYQSVHV-----PFTHSSR------SWAAFSSAPPSDLIS TURTRGS_195_1_PE4 NOISO FLLWLLDRVHEDLEGAARGLGSDKLQP-----EARKTSE--------DLPSPSAQLPLGQ XENTR255_2_PE8 NOISO FLLWLLDRVHEDLNATCGQKAKAKAGA-----EQNQ-----------VVHSQSPEPGGSQ MATCH CONSENSUS FLLWLLDR?HED????????????????????????????????????????????????

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS AILMEGL192808_1_1_PE2 ISOFORMIN SFVQSHFQAQYSPPRSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS ANOCA79_5_PE1 NOISO SFVQNHFQAQY---RSSLTCPHCL-KQSNTFDPFLCISLPIPLR-QTRPLNVTLVFQSKS BOVIN19_30_PE7 ENSBTAG00000000120 SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS BOVIN19_30_PE8 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS BOVIN19_30_PE9 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS CALJA5_74_PE21 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS CALJA5_74_PE22 ENSCJAG00000012679 SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS CANFA5_38_PE5 NOISO ------------------------------------------------------------ CHOHOGS_5925_PE2 NOISO SFVQSHFQAQY---XXXXXXXXXXXXX-XXXXXXXXXXXXXXXX-XXXFLSVTLVFPSKT CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 SFIQKLFQGHY---RSSLTCPNCK-KRSDTTDPFLCVSLPLKQR-TTRPMYANVVYLPNK DANRE3_27_PE27 NOISO SFVQEHFQAQY---KSSLTCPHCL-KQSNTFDPFLCISLPIPLR-QTRPVCVTLVFSSKG DANRE6_20_PE8 ENSDARG00000060632 SFVQEHFQAQY---RSSLTCPHCL-KQSNTFDPFLCISLPIPLR-QTRALNVTLVFSTEG DANRE6_20_PE9 ISOFORMIN SFVQEHFQAQY---RSSLTCPHCL-KQSNTFDPFLCISLPIPLR-QTRALNVTLVFSTEG DASNOGS_5350_PE2 NOISO SFVQSHFQAQYXXXXXXXXXXXXX----XXXXXXXXXXXXXXXX-XXXFLSVTLVFPSKS DIPORGS_4986_PE2 NOISO SFVQSHFQAQYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-----XXXXXXXXXXXX ERIEUGS_5458_PE1 NOISO SFVQSPFQAQYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----XXXXXXXXXXX- FELCAGS_3581_PE2 NOISO SFVQSHFQAQYXXXXXXXXXX----XXXXXXDPFLCVSLPIPLR-QTRFLSVTLVFPSKS FUGRU26_1_PE135 NOISO SFVQEHFQAQY---RSSLTCPHCL-KQSNTFDPFLCISLPIPLR-QTRSMCVTLVFNTKG FUGRU65_1_PE42 NOISO SFVKEHFQAQY---KSSLTCPHCR-KQSNTFDPFLCISLPIPLC-QTRLLHVILVFSNKG GASACGROUPIX_18_PE15 NOISO SFVQEHFQAQY---RSSLTCPHCL-KQSNTFDPFSCISLPIPLR-QTRPMCVTLVFTTKG GASACGROUPXI_4_PE54 ISOFORMIN SFVQEHFQAQY---KSSLTCPHCM-KQSNTFDPFLCISLPIPLR-QTRSLCVILVFSTKG GASACGROUPXI_4_PE55 ENSGACG00000006879 SFVQEHFQAQY---KSSLTCPHCM-KQSNTFDPFLCISLPIPLR-QTRSLCVILVFSTKG GORGO17_11_PE4 NOISO SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS HORSE11_55_PE3 NOISO SFVQSHFQAQY---RSSLTCPHCR-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFLSKS HS17_PE534 ENSG00000154914 SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS LOXAF47_14_PE7 ENSLAFG00000014238 SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLAFPSKS LOXAF47_14_PE8 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLAFPSKS MACEUGS_6207_PE1 NOISO XXXXXXXXXXXXXXXSSLTCPHCQ-KQSNTFDPFLCVSLPIPAC-QTRSLNVTLVFQSKS MACMU16_10_PE4 ENSMMUG00000014039 SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRYVSVAACPPIRR MICMUGS_287_PE4 NOISO SFVQSHFQAQY---XXXXXXPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLAFPSKG MONDO2_262_PE1 NOISO SFVQNHFQAQY---RSSLTCPHCQ-KQSNTFDPFLCVSLPIPSC-QTRSLNVTLVFQSKS MOUSE11_PE1552 ENSMUSG00000020905 SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS MOUSE11_PE1553 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS MOUSE11_PE1554 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS OCHPRGS_5271_PE1 NOISO SFVQSHFQAQY---RSSLTCPHCQ-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFLSKS ORYLA1_12_PE4 NOISO SFVQEHFQAQY---RSSLTCPHCL-KQSNTFDPFLCISLPIPLR-QTRALCVTLVFSAKG PANTR17_49_PE8 NOISO SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS PIG12_54_PE2 NOISO SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFLSQS PONPY17_11_PE3 NOISO SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS PROCAGS_5529_PE2 NOISO SFVQSHFQAQYXXXXXXXXXXXXX----XXXXXXXXXXXXXXXX-XXXFLSVTLVFSSKS PTEVAGS_2827_PE2 NOISO SFVQSHFQAQY---XXXXXXXXXXXXXXXXXXXHLCVSLPIPLR-QTRFLSITLVFPSKS RABIT19_11_PE2 NOISO SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFLSKS RAT10_PE1005 ENSRNOG00000003785 SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS RAT10_PE1006 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS RAT10_PE1007 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS RAT10_PE1008 ISOFORMIN SFVQSHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTRFLSVTLVFPSKS SORARGS_4441_PE1 NOISO SFVQSHFQAQYXXXXXXXXXXXXX----XXXXXXXXXXXXXXXX-XXXFLSITLAFPSKS TARSYGS_5169_PE1 NOISO SFVQNHFQAQY---RSSLTCPHCL-KQSNTFDPFLCVSLPIPLR-QTXXXXXXXXXXXXX TETNGUNRD_PE1034 NOISO DFVQEHFQAQY---RSSLTCPHCL-KQSNTFDPFLCISLPIPLR-QTRPMCVTLVFNAKG TETNGUNRD_PE266 NOISO VFYSNL--FRYGKPESSLTCPHCR-KQSNTFDPFLCISLPIPLC-QTRLLCVILVFSNKG TURTRGS_195_1_PE4 NOISO SFVQSHFQAQY---XXXXXXXXXX-XXXXXXXXXXXXXXXXXXX-XXXFLSVTLVFPSKS XENTR255_2_PE8 NOISO SFVQEHFQAQY---RYGPERP----RGSKFYCPPLRVSLSVPLSLPTRALNLTLVFQTKQ MATCH CONSENSUS SFVQ?HFQAQY?????????P??????S???DPFLC?SLPIP???QTR?L?VTLVF??K?

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 -----------QRFLRVGLAVPILSTVAALRKIVAEEGGVPADE-VILVELCPTGFQRSF AILMEGL192808_1_1_PE2 ISOFORMIN -----------QRFLRVGLAVPILSTVAALRKIVAEEGGVPADE-VILVELCPTGFQRSF ANOCA79_5_PE1 NOISO -----------QRFVRVGLTVPLFSTVAKLREMVAEEGKLSPDQ-VILAELGPSGFQCSF BOVIN19_30_PE7 ENSBTAG00000000120 -----------QRFLRIGLAVPILSTVAALRKMVAEEGGVSPEE-VILVELYPSGLQRSF BOVIN19_30_PE8 ISOFORMIN -----------QRFLRIGLAVPILSTVAALRKMVAEEGGVSPEE-VILVELYPSGLQRSF BOVIN19_30_PE9 ISOFORMIN -----------QRFLRIGLAVPILSTVAALRKMVAEEGGVSPEE-VILVELYPSGLQRSF CALJA5_74_PE21 ISOFORMIN -----------QRFLRVGLAVPILSTVAALRKMVAEEGGIPVDE-VILVELYPSGFQRSF CALJA5_74_PE22 ENSCJAG00000012679 -----------QRFLRVGLAVPILSTVAALRKMVAEEGGIPVDE-VILVELYPSGFQRSF CANFA5_38_PE5 NOISO --------------------------------MVSEEGDIPADE-VILVELCPNGFQRSF CHOHOGS_5925_PE2 NOISO -----------QQFLRVGLAIPILGTVAALRKMVAEEGDVPVDE-VILVEMDPSGFQRSF CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 RRSGIGRRHMAGKTIKIGVSVPLDGKIIDLRQAVAAECGIHSRL-LAFVDLQHDGFHKAY DANRE3_27_PE27 NOISO -----------QRYLRVGLAVPLFGSLACLRRMVADEGKIAPDQ-VILTEIYTTGFQRSF DANRE6_20_PE8 ENSDARG00000060632 -----------QRFLRIGLAVPLFGSISTLRAMVAEEGKLSPDQ-VILTEMNSGGFQRSF DANRE6_20_PE9 ISOFORMIN -----------QRFLRIGLAVPLFGSISTLRAMVAEEGKLSPDQ-VILTEMNSGGFQRSF DASNOGS_5350_PE2 NOISO -----------QRFLRVGLAVPILGTVAALRKTVGEEGNVPADE-VILVEMHPGGFQRSF DIPORGS_4986_PE2 NOISO -----------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-VILVELYPSGFQRSF ERIEUGS_5458_PE1 NOISO -----------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-XXXXXXXXXXXXXXX FELCAGS_3581_PE2 NOISO -----------QRFLRVGLAVPILSTVASLRKMVAEEGGVPADE-VILVELCPSGFQRSF FUGRU26_1_PE135 NOISO -----------QRYLRVGLAVPLFGSVSNLRSMVAEEGSISSNQ-VILAELYSSGFQRSF FUGRU65_1_PE42 NOISO -----------QRYLRVKVAVPLFGSVACLRRMVADEGKLSPDQ-VILTEVYSKGFCRSF GASACGROUPIX_18_PE15 NOISO -----------HRYLRVGLAVPLLGSLSCLRAMVAQEGNISPDQ-VILSELYSTGFQRSF GASACGROUPXI_4_PE54 ISOFORMIN -----------QRYLRVGLAVPLFGSVAFLRRMAADEGKISPDQ-VILTEVYSTGFQRSF GASACGROUPXI_4_PE55 ENSGACG00000006879 -----------QRYLRVGLAVPLFGSVAFLRRMAADEGKISPDQQVILTEVYSTGFQRSF GORGO17_11_PE4 NOISO -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPADE-VILVELYPSGFQRSF HORSE11_55_PE3 NOISO -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPVDE-VILVELHPSGLQRSF HS17_PE534 ENSG00000154914 -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPADE-VILVELYPSGFQRSF LOXAF47_14_PE7 ENSLAFG00000014238 -----------QRFLRVGLAVPILSTVAALRKMVADEGNVPADE-VILVELHPSGFHRSF LOXAF47_14_PE8 ISOFORMIN -----------QRFLRVGLAVPILSTVAALRKMVADEGNVPADE-VILVELHPSGFHRSF MACEUGS_6207_PE1 NOISO -----------QRFLRIGLAVPLFSTVATLRKMVAEEGKITANE-VVLVELSPSGFQRSF MACMU16_10_PE4 ENSMMUG00000014039 -----------KERVKMGISYLLISLSEIK------RENDLSFQ-VILVELYPSGFQRSF MICMUGS_287_PE4 NOISO -----------QQFLRVGLAVPILSTVAALRKMVAEEGGVPADE-XXXXXXXXXXXXXXX MONDO2_262_PE1 NOISO -----------QMFLRIGLAVPLFSTVATLRKMIAEEGKISANE-VVLVELHPSGFQRSF MOUSE11_PE1552 ENSMUSG00000020905 -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPAEE-VILVELYPNGFQRSF MOUSE11_PE1553 ISOFORMIN -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPAEE-VILVELYPNGFQRSF MOUSE11_PE1554 ISOFORMIN -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPAEE-VILVELYPNGFQRSF OCHPRGS_5271_PE1 NOISO -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPVDE-VILVELHPSGFQ-SF ORYLA1_12_PE4 NOISO -----------QRYVRVGLAVPLFGSISTLRTMTAAEGNIAPDQ-VILAELYSTGFQRSF PANTR17_49_PE8 NOISO -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPADE-VILVELYPSGFQRSF PIG12_54_PE2 NOISO -----------QRFLRVGLAVPILSTVAALRKMVAEEGGIPAAE-VILVELYPNGLQRSF PONPY17_11_PE3 NOISO -----------QRFLRVGLAVPILSTVAALRKMVAEEGGIPADE-VILVELYPSGFQRSF PROCAGS_5529_PE2 NOISO -----------QRFLRVGLAVPILSTVVALRRMVADEGNIPADE-XXXXXXXXXXXXXXX PTEVAGS_2827_PE2 NOISO -----------QQFLRVGLAVPILSTVAALRKMVAEEGRVPVDE-VILVELYPSGLQRSF RABIT19_11_PE2 NOISO -----------QRFLRVGLAVPILSTVAALRKMVAEEGGIPVDE-VILVELYPSGFQRSF RAT10_PE1005 ENSRNOG00000003785 -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPAEE-VILVELYPNGFQRSF RAT10_PE1006 ISOFORMIN -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPAEE-VILVELYPNGFQRSF RAT10_PE1007 ISOFORMIN -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPAEE-VILVELYPNGFQRSF RAT10_PE1008 ISOFORMIN -----------QRFLRVGLAVPILSTVAALRKMVAEEGGVPAEE-VILVELYPNGFQRSF SORARGS_4441_PE1 NOISO -----------QKFLRVGLAVPILSTVAALRRMVAEEGGAPVHE-VILVELCPTGLQRSF TARSYGS_5169_PE1 NOISO -----------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEAP-VILVELYPNGFQRSF TETNGUNRD_PE1034 NOISO -----------QRYLRVGLAVPLFGSVSNLRSMVAEEGSISSNQ-VILTELYSTGFQRSF TETNGUNRD_PE266 NOISO -----------QRYLRIRLAVPLFGSVACLRRMVADEGKLSPDQ-VNRAEIERDGARTRQ TURTRGS_195_1_PE4 NOISO -----------QQSLRVGLAVPILSTVAALRKMVAEEGGIPVDE-XXXXXXXXXXXXXXX XENTR255_2_PE8 NOISO -----------QRFVRVGLAVPLLGTVTSLRKMVADEGNMAPDQ-VILVELYPSGFQRSF MATCH CONSENSUS ???????????Q??LR?GLAVP???????LR?MVA?EG???????VIL?E????G?QRSF

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 FDEEDLNT--------ISEGDNVYAFHAPPP---PGQEMLSAHP---------------- AILMEGL192808_1_1_PE2 ISOFORMIN FDEEDLNT--------ISEGDNVYAFHAPPP---PGQEMLSAHP---------------- ANOCA79_5_PE1 NOISO SDDEDLNT--------IADGDSIYAFQAPLS---FSKAS-SSRL---------------- BOVIN19_30_PE7 ENSBTAG00000000120 FDEEDLNT--------IAEGDNVYAFQAPPS---PGQEMLSARP---------------- BOVIN19_30_PE8 ISOFORMIN FDEEDLNT--------IAEGDNVYAFQAPPS---PGQEMLSARP---------------- BOVIN19_30_PE9 ISOFORMIN FDEEDLNT--------IAEGDNVYAFQAPPS---PGQEMLSVQN---------------- CALJA5_74_PE21 ISOFORMIN FDEEDLNT--------IAEGDNVYAFQVPPS---PSQGTLSAHP---------------- CALJA5_74_PE22 ENSCJAG00000012679 FDEEDLNT--------IAEGDNVYAFQVPPS---PSQGTLSAHP---------------- CANFA5_38_PE5 NOISO FDEEDLNT--------ISEGDNVYAFHAPPP---PGQETLSAHP---------------- CHOHOGS_5925_PE2 NOISO FDEEDLNT--------IAEGDNVYAFHTPPS---PGQGTLSARP---------------- CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 GDDYSLSDFPINNSSTTAANMSLYAFEMPPIAKMTA-G---------------------- DANRE3_27_PE27 NOISO FDDDDLTI--------IAESDIIYAFQASPL---YIRGGSARIS---------------- DANRE6_20_PE8 ENSDARG00000060632 SDEDDMTS--------ISESDVIYAFQVPPH---FTRGGSMRFS---------------- DANRE6_20_PE9 ISOFORMIN SDEDDMTS--------ISESDVIYAFQVPPH---FTRGGSMRFS---------------- DASNOGS_5350_PE2 NOISO FDEEDLNT--------IAEGDDVYAFHAPPS---PGQGTLSAHP---------------- DIPORGS_4986_PE2 NOISO FDEEDLNS--------IAEGDNVYAFQAPPS---PGLGTLSAHP---------------- ERIEUGS_5458_PE1 NOISO XXXXXXXX--------XXXXXXXXXXXXXXX---XXXXXXXXRL---------------- FELCAGS_3581_PE2 NOISO FDEEDLNT--------IAEGDNVYAFHAPPP---PGQEMLSAHP---------------- FUGRU26_1_PE135 NOISO SDEDDLTA--------IGDSDVVYAFQAPPL---HSHGSSASSH---------------- FUGRU65_1_PE42 NOISO FDEEDLAS--------IAEHDVIYAFQAPAI---YIRGGSARIS---------------- GASACGROUPIX_18_PE15 NOISO ADDDDLTA--------ITDSDVIYAFQAPPP---HSRGGSVPHS---------------- GASACGROUPXI_4_PE54 ISOFORMIN FDEEDLTS--------IAENDVIYAFQAPPL---YIRGGSARIS---------------- GASACGROUPXI_4_PE55 ENSGACG00000006879 FDEEDLTS--------IAENDVIYAFQAPPL---YIRGGSARIS---EIRINGRLNIEFH GORGO17_11_PE4 NOISO FDEEDLNT--------IAEGDNVYAFQVPPS---PSQGTLSAHP---------------- HORSE11_55_PE3 NOISO FDEEDLNT--------IAEGDSIYAFQAPPP---TSRELLLARP---------------- HS17_PE534 ENSG00000154914 FDEEDLNT--------IAEGDNVYAFQVPPS---PSQGTLSAHP---------------- LOXAF47_14_PE7 ENSLAFG00000014238 FDDDDLNS--------IAEGDNVYAFHCPSS---PGQGRRSVHP---------------- LOXAF47_14_PE8 ISOFORMIN FDDDDLNS--------IAEGDNVYAFHCPSS---PGQGRRSVHP---------------- MACEUGS_6207_PE1 NOISO FDDEDLNT--------ISEGDNVYAFQAPWS---PIWGVPTTFS---------------- MACMU16_10_PE4 ENSMMUG00000014039 FDEEDLNT--------IAEGDNVYAFQVPPS---PSQGTLSAHP---------------- MICMUGS_287_PE4 NOISO XXXXXXXX--------XXXXXXXXXXXXXXX---XXXXXXXXHA---------------- MONDO2_262_PE1 NOISO FDEEDLNC--------ISDGDNVYAFQAPLS---PIWGVPASF----------------- MOUSE11_PE1552 ENSMUSG00000020905 FDEEDLNT--------IAEGDNVYAFQVPPS---PGLGTLSAHP---------------- MOUSE11_PE1553 ISOFORMIN FDEEDLNT--------IAEGDNVYAFQVPPS---PGLGTLSAHP---------------- MOUSE11_PE1554 ISOFORMIN FDEEDLNT--------IAEGDNVYAFQVPPS---PGLGTLSAHP---------------- OCHPRGS_5271_PE1 NOISO FDDEDLNT--------IAEGDNVYAFQVPPS---PGQGTLSAHP---------------- ORYLA1_12_PE4 NOISO SDEDELTM--------IADSDVVYAFQAPPV---SNRGGLTPHS---------------- PANTR17_49_PE8 NOISO FDEEDLNT--------IAEGDNVYAFQVPPS---PSQGTLSAHP---------------- PIG12_54_PE2 NOISO FDEEDLNT--------IAEGDNVYAFQAPPS---PGQEMLSACP---------------- PONPY17_11_PE3 NOISO FDEEDLNT--------IAEGDNVYAFQVPPS---PSPGTLSAHP---------------- PROCAGS_5529_PE2 NOISO XXXXXXXX--------XXXXXXXXXXXXXXX---XXXXX-XX------------------ PTEVAGS_2827_PE2 NOISO FDEEDLNT--------ITEGDNVYAFLAPPP---TGQEISSACP---------------- RABIT19_11_PE2 NOISO FDEEDLNS--------IAEGDNVYAFQACLA---PGQGTLAVHP---------------- RAT10_PE1005 ENSRNOG00000003785 FDEEDLNT--------IAEGDNVYAFQVPPL---PGPGTLAAHP---------------- RAT10_PE1006 ISOFORMIN FDEEDLNT--------IAEGDNVYAFQVPPL---PGPGTLAAHP---------------- RAT10_PE1007 ISOFORMIN FDEEDLNT--------IAEGDNVYAFQVPPL---PGPGTLAAHP---------------- RAT10_PE1008 ISOFORMIN FDEEDLNT--------IAEGDNVYAFQVPPL---PGPGTLAAHP---------------- SORARGS_4441_PE1 NOISO FDEDDLNT--------ISEGDSVYAFQTSLP---PVQEITAVQP---------------- TARSYGS_5169_PE1 NOISO FDEEDLNT--------ITEGDNVYAFQVPPS---PGQGTLSAHP---------------- TETNGUNRD_PE1034 NOISO SDEDDLTA--------IADSDVVYAFQAPPL---CSLGSSTSSH---------------- TETNGUNRD_PE266 NOISO -------G--------VSDSDAEKTFIQRVCILVTGKENILLHQFIYQKRVGVNQNFHIK TURTRGS_195_1_PE4 NOISO XXXXXXXX--------XXXXXXXXXXXXXXX---XXXXXXXXRP---------------- XENTR255_2_PE8 NOISO SDDEDLNS--------IAEGDPVYAFQAPMP---IPKASGPSRP---------------- MATCH CONSENSUS ?D??DL??????????I???D??YAF??P???????????????????????????????

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 ---SGLSVSPRLAAR-----------EGPRSSLPVHS-ENKVLVLFCNLVGSGQQAS--- AILMEGL192808_1_1_PE2 ISOFORMIN ---SGLSVSPRLAAR-----------EGPRSSLPVHS-ENKVLVLFCNLVGSGQQAS--- ANOCA79_5_PE1 NOISO ---SGMSGAKGLCES-----------KSQMLSLLLHL-CGRILLLVCNVAGTGQQAV--- BOVIN19_30_PE7 ENSBTAG00000000120 ---SGLLVSPCLAAR-----------EGQRLSLPVHN-ETSVLILFCNLVGSGQQAS--- BOVIN19_30_PE8 ISOFORMIN ---SGLLVSPCLAAR-----------EGQRLSLPVHN-ETSVLILFCNLVGSGQQAS--- BOVIN19_30_PE9 ISOFORMIN ---G-CWS------------------------------------NLCLQESYGSRIS--- CALJA5_74_PE21 ISOFORMIN ---LGLSLSPRLAAR-----------ESQRFSLSLHS-ESKVLILFCNLVGSGQQAS--- CALJA5_74_PE22 ENSCJAG00000012679 ---LGLSLSPRLAAR-----------ESQRFSLSLHS-ESKVLILFCNLVGSGQQAS--- CANFA5_38_PE5 NOISO ---CGLSVSPRLAAR-----------EGQRVPLSVPS-KNKVLILICNLVGSGQQAS--- CHOHOGS_5925_PE2 NOISO ---SGLPVSPRLAAR-----------EGQWLSLPLQS-ENKVLILFCNLVGSGQQASXXX CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 --------------------------TLPRNFASSHK---KRATL---GGKFGKEG---- DANRE3_27_PE27 NOISO GFHHSLPSSPYS-SSGPEGHRLAASGALSSEFLNQTDGMLKVLLLVCNAAGAGQQA---- DANRE6_20_PE8 ENSDARG00000060632 GYHHSLPSSPYT--ADFKGHRLPPSGTLSTEFLNHGG-SSKVLLLICNVAGSGQQA---- DANRE6_20_PE9 ISOFORMIN ---------------DFKGHRLPPSGTLSTEFLNHGG-SSKVLLLICNVAGSGQQA---- DASNOGS_5350_PE2 NOISO ---PGLSVSPRLAAR-----------EGRRLSLPVQS-ENKVLILFCNLVGSGQQAS--- DIPORGS_4986_PE2 NOISO ---SGLSVSPRLVAR-----------EGQRLSLSHHS-ENKVLILFCNLVGSGQQAS--- ERIEUGS_5458_PE1 NOISO ---HGLPVSPRLATR-----------EGLQLALPIHS-ENKVLILFCNLVGSGQQAS--- FELCAGS_3581_PE2 NOISO ---PGLSISPRLAAH-----------GGQRLPPPVQS-ENKVLILLCNLAGSGQQAS--- FUGRU26_1_PE135 NOISO ---SGKLLSDCSTAS-PEGQRLPPSGTLSSDYLNQGG-SSKVLLLICNTAGGGQQAV--- FUGRU65_1_PE42 NOISO ---GGLPSSPYS--TGPMGQRLLPSGTLSSDFLNQGV-SVKILLLVCNAAGAGQHV---- GASACGROUPIX_18_PE15 NOISO ---ENLF--------------ARAGRTTRREYLNQGG-STKVLLLVCNTAGSGQQAV--- GASACGROUPXI_4_PE54 ISOFORMIN -----------------------------------GV-PVKILLLVCNAAGGGQQAV--- GASACGROUPXI_4_PE55 ENSGACG00000006879 GYHHSLPSSPYC--TGPEGQRLPSSGTVSSEFLNQGV-PVKILLLVCNAAGGGQQAV--- GORGO17_11_PE4 NOISO ---LGLSASPRLAAR-----------EGQRFSLSLHS-ESKVLILFCNLVGSGQQAS--- HORSE11_55_PE3 NOISO ---PGLSVSPHLAAR-----------EGQRASLPAHG-ENKVLLLFCNVVGSGLQAS--- HS17_PE534 ENSG00000154914 ---LGLSASPRLAAR-----------EGQRFSLSLHS-ESKVLILFCNLVGSGQQAS--- LOXAF47_14_PE7 ENSLAFG00000014238 ---SGLPVSPRLAAR-----------EGQR--------GNKVLILCCNVVGSGQQAS--- LOXAF47_14_PE8 ISOFORMIN ---SGLPVSPRLAAR-----------EGQR--------GNKVLILCCNVVGSGQQAS--- MACEUGS_6207_PE1 NOISO -------------------------------SLSSGN-GSKVLLLLCNLVGSGQKAK--- MACMU16_10_PE4 ENSMMUG00000014039 ---LALSVSPRLAAR-----------EGQRFSLSLHS-ESKVLILFCNLVGSGQQAS--- MICMUGS_287_PE4 NOISO ---SGLPVSPRLAAH-----------EGQRFPLSLHS-ENKVLILFCNLVGSGQQAS--- MONDO2_262_PE1 NOISO ---SGIVVPSTL----------------STESLTSGS-GSKVLILLCNLVGSGHKAK--- MOUSE11_PE1552 ENSMUSG00000020905 ---SGLSVSPRLPVR-----------DSQRFSGPLHS-ENRVVFLFCNLVGSGQQAS--- MOUSE11_PE1553 ISOFORMIN ---SGLSVSPRLPVR-----------DSQRFSGPLHS-ENRVVFLFCNLVGSGQQAS--- MOUSE11_PE1554 ISOFORMIN ---SGLSVSPRLPVR-----------DSQRFSGPLHS-ENRVVFLFCNLVGSGQQAS--- OCHPRGS_5271_PE1 NOISO ---PALSVSPRLAAR-----------EAPRPSLPLRS-ENKVLILLCNLVGSGQQAS--- ORYLA1_12_PE4 NOISO ---ASPPLLTPP------RL-GLQSGRLSSEYLNHSS-SSKVILLMCNTVGSGQHAA--- PANTR17_49_PE8 NOISO ---LGLSASPRLAAR-----------EGQRFSLSLHS-ESKVLILFCNLVGSGQQAS--- PIG12_54_PE2 NOISO ---PGLPVSPRLAAR-----------EGQRLSRPVPG-ENKVLILFCNLVGSGQQAS--- PONPY17_11_PE3 NOISO ---LGLSVSPRLAAR-----------EGQRFSLSLHS-ESKVLILFCNLVGSGQQAS--- PROCAGS_5529_PE2 NOISO ---XXXXXX-XX----------------XXXXXXXXX-XXXXXXXXXXXXXXXXXXXXXX PTEVAGS_2827_PE2 NOISO ---PGLSVSPRLAAR-----------ESQRLSLPLHS-ENKVLILFCNLVGSGQQAS--- RABIT19_11_PE2 NOISO ---PGLSISPRLAAR-----------EGQRPGLPLHS-ENKVLILFCNLVGSGQQAS--- RAT10_PE1005 ENSRNOG00000003785 ---PGLSVSPRLAVR-----------DSQRFSVPLHS-ENRVLILFCNLVGSGQQAS--- RAT10_PE1006 ISOFORMIN ---PGLSVSPRLAVR-----------DSQRFSVPLHS-ENRVLILFCNLVGSGQQASRNN RAT10_PE1007 ISOFORMIN ---PGLSVSPRLAVR-----------DSQRFSVPLHS-ENRVLILFCNLVGSGQQAS--- RAT10_PE1008 ISOFORMIN ---PGLSVSPRLAVR-----------DSQRFSVPLHS-ENRVLILFCNLVGSGQQAS--- SORARGS_4441_PE1 NOISO ---SGLPVSPSMVAC-----------EGQQLSMPIYN-KNKVLILFCNLVGSGQLAT--- TARSYGS_5169_PE1 NOISO ---SGLSVSPLLAAR-----------ESQRFSLSLHS-ENKVLILFCNLVGSGQQAS--- TETNGUNRD_PE1034 NOISO ---SVYSVRPQP------FLVFPIQDIYKFSPPIQTG-SPKVLLLVCNTAGSGQQAV--- TETNGUNRD_PE266 NOISO EIVMGLSFHH----LHTPRDQLLPSGTLSSEFLNQGV-PVKILLLVCNAAGAGQHAV--- TURTRGS_195_1_PE4 NOISO ---SGLPVSPRLAAC-----------EGQRLSLPVHS-ETTVLILFCNLVGSGQQAS--- XENTR255_2_PE8 NOISO ---TGYPRSLPSSPRYPDPEAQKFSGAGSKSSEFLAD-PDRILLLLCNTEGSGKQAN--- MATCH CONSENSUS ?????????????????????????????????????????VL?L?CN??GSGQQA????

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 ------RFGPPFLLREDRAISWAQLRECILSKV---RCVMKSEAPIQNPG-SLFSIRVVG AILMEGL192808_1_1_PE2 ISOFORMIN ------RFGPPFLLREDRAISWAQLRECILSKV---RCVMKSEAPIQNPG-SLFSIRVVG ANOCA79_5_PE1 NOISO ------RFGPPLLMREDRAMSWDQLQQCILGKM---RYLMRREAQVQATG-NLFQVRVLG BOVIN19_30_PE7 ENSBTAG00000000120 ------RFGPPFLVREDRAISWIQLQQCILSKV---RYLMKSEVPIQNLG-SLFSIRVVG BOVIN19_30_PE8 ISOFORMIN ------RFGPPFLVREDRAISWIQLQQCILSKV---RYLMKSEVPIQNLG-SLFSIRVVG BOVIN19_30_PE9 ISOFORMIN ------RFGPPFLVREDRAISWIQLQQCILSKV---RYLMKSEVPIQNLG-SLFSIRVVG CALJA5_74_PE21 ISOFORMIN ------RFGPPFLIREDRAISWAQLQQSILSKV---CHLMKSEAPVQNLG-SLFSIRVVG CALJA5_74_PE22 ENSCJAG00000012679 ------RFGPPFLIREDRAISWAQLQQSILSKV---CHLMKSEAPVQNLG-SLFSIRVVG CANFA5_38_PE5 NOISO ------RFGPPFLLREDRAITWVQLQQRILSNV---RCVMKSEVPIQNPG-SLFSVRVVG CHOHOGS_5925_PE2 NOISO XXXXXXXXXXXX---------XXXXXXXXXXXX---XXXXXXXXXXXNLG-SLFSIRVVG CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 -----KRFGRPLVFRASRDVLSTELQCMILNQM---SDQLKNGVKHERMG-SLFRLQVKD DANRE3_27_PE27 NOISO -----VRFGPPFLMREDRSVSWDQLQQSILSKL---YYLMINGSQAHNFR-VLFKIRVVG DANRE6_20_PE8 ENSDARG00000060632 -----TRFGPPFLMRVERTLSWDHLQQSVCNKL---YVFQKTAIIK--TT-ELFKVRVVG DANRE6_20_PE9 ISOFORMIN -----TRFGPPFLMRVERTLSWDHLQQSVCNKL---YVFQKTAIIKVSTT-ELFKVRVVG DASNOGS_5350_PE2 NOISO ------RFGPPFLIREDRGISWAQLQQRVLSKI---RYLMKSEPPLQNLG-SLFSIRVVG DIPORGS_4986_PE2 NOISO ------RFGPPFLIREDRTISWAQLQQCILSKV---CCLMRTEASAQNLG-SLFSIRVVG ERIEUGS_5458_PE1 NOISO ------RFGPPFLIREDRTVSWAQLQQCILNKV---HYLMKNEAPIQNSG-PLFSIRVVG FELCAGS_3581_PE2 NOISO ------RFGPPFLIREDRAISWVQLQQCILSKV---RYLMKSEAPLQNPG-SLFSIRVVG FUGRU26_1_PE135 NOISO ------RFGPPFLMHEDRNISWEQLQQSILSKL---YYLMLNGSQAQNAG-KLFRIRVVG FUGRU65_1_PE42 NOISO -----VRFGPPFLMREDRSISWDQLQQSILSKL---YYLMLSGTQAQNTG-VLFNIRVVG GASACGROUPIX_18_PE15 NOISO ------RFGPPFLMREDRSVSWDQLQQSLLSKL---YYLMLNGSQAQNAG-VLFKIRVVG GASACGROUPXI_4_PE54 ISOFORMIN -----RRFGPPFLMREDRSISWDQLQQSILISSTAVMYSMKMFFFFQNTR-VLFNVRVVG GASACGROUPXI_4_PE55 ENSGACG00000006879 ------RFGPPFLMREDRSISWDQLQQSILSKL---YYLMINGAQAQNTR-VLFNVRVVG GORGO17_11_PE4 NOISO ------RFGPPFLIREDRAVSWAQLQQSILSKV---RHLMKSEAPVQNLG-SLFSIRVVG HORSE11_55_PE3 NOISO ------RFGPPFLMREDRTISWVQLQQCILSKV---RYLMKSEAPIQNPG-SMFSIRVVG HS17_PE534 ENSG00000154914 ------RFGPPFLIREDRAVSWAQLQQSILSKV---RHLMKSEAPVQNLG-SLFSIRVVG LOXAF47_14_PE7 ENSLAFG00000014238 ------RFGPPFLVREDRGISWPQLQQCILSKV---CYLMKREAPLQNLG-VPFSIRVVG LOXAF47_14_PE8 ISOFORMIN ------RFGPPFLVREDRGISWPQLQQCILSKV---CYLMKREAPLQNLG-VPFSIRVVG MACEUGS_6207_PE1 NOISO ------RFGPPLLMREDRTISWDQLQQQVFNKM---RYLMKQEVPVQSLE--MFAIRVVG MACMU16_10_PE4 ENSMMUG00000014039 ------RFGPPFLIREDRAVSWAQLQQSILSKV---RHLMKSEAPVQNLG-SLFSIRVVG MICMUGS_287_PE4 NOISO ------RFGPPFLIREDRAISWPQLQQSILSKV---RYLMKNEASVQNLG-SLFSIRVVG MONDO2_262_PE1 NOISO ------RFGPPLLMREDRAISWDQFQQQVFSTI---QCLMKKEVSLQSLG-SLFAVRVVG MOUSE11_PE1552 ENSMUSG00000020905 ------RFGPPFLIREDRTISWAQLQQCILSKV---RCLMRSEVSAQDLG-TLFSIRVVG MOUSE11_PE1553 ISOFORMIN ------RFGPPFLIREDRTISWAQLQQCILSKV---RCLMRSEVSAQDLG-TLFSIRVVG MOUSE11_PE1554 ISOFORMIN ------RFGPPFLIREDRTISWAQLQQCILSKV---RCLMRSEVSAQDLG-TLFSIRVVG OCHPRGS_5271_PE1 NOISO ------RFGPPFLIMEDRTVSWAQLQQCVLSKV---RYLMRSEAPVQNLG-SLFSVRVVG ORYLA1_12_PE4 NOISO ------RFGPPFLMVEDRTVSWDQLTQSILSNL---YYLMANASQSQTAG-VLFKIRVVG PANTR17_49_PE8 NOISO ------RFGPPFLIREDRAVSWAQLQQSILSKV---RHLMKSEAPVQNLG-SLFSIRVVG PIG12_54_PE2 NOISO ------RFGPPFLIREDRAISWAQLQQSILNKV---RYLMKGEAPVQNPG-SLFSIRVVG PONPY17_11_PE3 NOISO ------RFGPPFLIREDRAVSWAQLQQSILSKV---RHLMKSEAPLQNLG-SLFSIRVVG PROCAGS_5529_PE2 NOISO XXXXXXXFGPPFLVREDRGISWPQLQQCILSRV---CCLMKNEAPMQXXX-XXXXXXXXX PTEVAGS_2827_PE2 NOISO ------RFGPPFLMREDRTISWVQLQQCILSKV---QYLMKNEAPIQNPG-SLFSIRVVG RABIT19_11_PE2 NOISO ------RFGPPFLIREDRSISWAQLQQCILSKV---RCLMRSEAPVQNLG-SLFSIRVVG RAT10_PE1005 ENSRNOG00000003785 ------RFGPPFLIREDRTISWAQLQQCILSKV---RCLMRNEVSAQDLG-TLFSIRVVG RAT10_PE1006 ISOFORMIN LFPWIFRFGPPFLIREDRTISWAQLQQCILSKV---RCLMRNEVSAQDLG-TLFSIRVVG RAT10_PE1007 ISOFORMIN ------RFGPPFLIREDRTISWAQLQQCILSKV---RCLMRNEVSAQDLG-TLFSIRVVG RAT10_PE1008 ISOFORMIN ------RFGPPFLIREDRTISWAQLQQCILSKV---RCLMRNEVSAQDLG-TLFSIRVVG SORARGS_4441_PE1 NOISO ------RFGPPFLVREDRAISWGQLQQCILSKV---HPLMKSKASVQNLG-SLFSIRVVG TARSYGS_5169_PE1 NOISO ------RFGPPFLIREDRAISWAQLQQSVLSKV---RYLMKSEAPVQXXX-XXXXXXXXX TETNGUNRD_PE1034 NOISO ------RFGPPFLMLEDRNISWEQLQQSILSKL---YYLMLNGSQAQDLL--LLKIRIIL TETNGUNRD_PE266 NOISO ------RFGPPFLMREDRSISWDQLQQSILSKM---YYLMVNGTQAQIILLVQYHISFIV TURTRGS_195_1_PE4 NOISO ------RFGPPFLIREDRAISWVQLHQCILSKV---RYLMKSEASVQNLG-SLFSIRVVG XENTR255_2_PE8 NOISO ------RFGPPLLVREDSTLSWEQLQQCILGKL---RYLLRNEALLQQNGTALFHIRVVG MATCH CONSENSUS ??????RFGPPFL?REDR??SW?QLQQ?ILSK??????LM??????Q??G??LF??RVVG

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 LSLAC-TYLSPQDSHPLCHWAVDRALYPRRPGGPPHIKLAVEWDSCAKERL--FGGLQRE AILMEGL192808_1_1_PE2 ISOFORMIN LSLAC-TYLSPQDSHPLCHWAVDRALYPRRPGGPPHIKLAVEWDSCAKERL--FGGLQRE ANOCA79_5_PE1 NOISO GSTLC-SYLSPQDSWPLYHPAVDRVLQFSGPGGPAHVKLVIEWDVSTKESL--FGNIQEE BOVIN19_30_PE7 ENSBTAG00000000120 LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPPHIKLAVEWDVDTKERL--FGSPQEE BOVIN19_30_PE8 ISOFORMIN LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPPHIKLAVEWDVDTKERL--FGSPQEE BOVIN19_30_PE9 ISOFORMIN LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPPHIKLAVEWDVDTKERL--FGSPQEE CALJA5_74_PE21 ISOFORMIN LSVAC-SYLSPKDSRPLCHWAVDRALHLRRPGGPPHVKLAVEWDGSVKERL--FGNLQEE CALJA5_74_PE22 ENSCJAG00000012679 LSVAC-SYLSPKDSRPLCHWAVDRALHLRRPGGPPHVKLAVEWDGSVKERL--FGNLQEE CANFA5_38_PE5 NOISO LSLAC-SYLSPHDSHPLCHWAVDRAMHLRRPGGPPHIKLAVEWESCAKERL--FGNLQEE CHOHOGS_5925_PE2 NOISO LSLIC-SYLSPQDSQPLCHWAVDRALHLRKPGGPPHVKLAVEWENSAVQRL--FGSPEEE CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 GIPNK-SYLSQQAQQPLRLPTVDKALENCPAGGPLHIKLVAEWDTDTKFFISTF------ DANRE3_27_PE27 NOISO GSASY-SYLNPQDGRPLFHPAVDRALKLCGSGGPPHVKLIVEWEHRIKDCL--FGNIQEE DANRE6_20_PE8 ENSDARG00000060632 GSASC-SYLSPHDSRPLCHPTVDRALKLCGPGGPLHVKLIIEWDNKIKECL--FGNIQEE DANRE6_20_PE9 ISOFORMIN GSASC-SYLSPHDSRPLCHPTVDRALKLCGPGGPLHVKLIIEWDNKIKECL--FGNIQEE DASNOGS_5350_PE2 NOISO FPLTC-SYLSPQDSRPLGHWAVDRALHLRRSGGLSHV-LAVEWDSGAKERL--FGSPPEE DIPORGS_4986_PE2 NOISO LSLAC-SYLSPQDSCPLCHWAVDRALHIRKPGGPPHIKLAVEWDSSIKERL--FGSLQEE ERIEUGS_5458_PE1 NOISO PSVPC-SYLSPQDSQPLCHWAVDRALHFRRPGGPPHVKLAVEWDSCVKERL--FGSLQEE FELCAGS_3581_PE2 NOISO LSLAC-SYLSPQDSHPLSHWAVDXXXXXXXXXXXXXXXXXXXXXXXXXXXX--XXXXXXX FUGRU26_1_PE135 NOISO GSAAY-SYLSPQDSRPLHHPAVDRALKLCGTGGPPHTKLVIEWENKTKECL--FGNIQEE FUGRU65_1_PE42 NOISO GLGFY-NYLSPEDKLPLYHPAVEGALKRCGPGGPPHVKLIIEWEHRIKDCL--FGNIQEE GASACGROUPIX_18_PE15 NOISO GSAAY-SYLSPHDSRPLSHPAVDRALKFCGPGGPAHVKIVIEWNNKTKECI--FS--KEE GASACGROUPXI_4_PE54 ISOFORMIN GSAFY-SYLSPQDGRPLFHPAVDRALKLCCSGGPPHVKLIIEWEHRIKDC-----NIQEE GASACGROUPXI_4_PE55 ENSGACG00000006879 GSAFY-SYLSPQDGRPLFHPAVDRALKLCCSGGPPHVKLIIEWEHRIKDCL--FGNIQEE GORGO17_11_PE4 NOISO LSVAC-SYLSPKDSRPLCHWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERL--FGSLQEE HORSE11_55_PE3 NOISO LSLAC-SYLSAQDGRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSGAKERL--FGSLQEE HS17_PE534 ENSG00000154914 LSVAC-SYLSPKDSRPLCHWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERL--FGSLQEE LOXAF47_14_PE7 ENSLAFG00000014238 LSLAC-SYLSPQDGRPLCHWVVDRALHLRRPGGPPHVKLAVEWDSCAKERL--FGSLQEE LOXAF47_14_PE8 ISOFORMIN LSLAC-SYLSPQDGRPLCHWVVDRALHLRRPGGPPHVKLAVEWDSCAKERL--FGSLQEE MACEUGS_6207_PE1 NOISO LSNIC-SY-SPQDSRPLFHWAVERSLKLSGPGGPPHIKLAVEWDRTTKELL--FGNIQEE MACMU16_10_PE4 ENSMMUG00000014039 LSVAC-SYLSPKDSRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVKERL--FGNLQEE MICMUGS_287_PE4 NOISO LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPPHIKLAVEWDSSAKERL--FGSLQEE MONDO2_262_PE1 NOISO LSNVC-SYLSPQDGRPLFHWAVERSLKLSRPGGPPHIKLAIEWDKTTKELF--FGNIQEE MOUSE11_PE1552 ENSMUSG00000020905 LSLAC-SYLSPKDNRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERL--FGSLQEE MOUSE11_PE1553 ISOFORMIN LSLAC-SYLSPKDNRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERL--FGSLQEE MOUSE11_PE1554 ISOFORMIN LSLAC-SYLSPKDNRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERL--FGSLQEE OCHPRGS_5271_PE1 NOISO LSLAC-SYLSPQDSRPLCHWEVDRALHLRRPGGPPHIKLAVEWDSSAKERL--FGNIPEE ORYLA1_12_PE4 NOISO SSAAY-SYLSPQDSRPLCHPAVDRALKLCGPGGPLHVKLVIEWERSTKEHL--FGSIQEE PANTR17_49_PE8 NOISO LSVAC-SYLSPKDSRPLCHWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERL--FGSLQEE PIG12_54_PE2 NOISO LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPSHVKLAVEWDSGTQERL--FGSVQEE PONPY17_11_PE3 NOISO LSVAC-SYLSPKDSRPLCHWAVDRVLHLRRPGGPPHVKLAVEWDSSVKERL--FGSLQEE PROCAGS_5529_PE2 NOISO XXXXX-XXXXXXXXXXXXXXXXXXALHLRKPGGPPHVKLAVEWDSCAKEXX--XXXXXXX PTEVAGS_2827_PE2 NOISO QSLAY-GYLPPQHSQPLCHWAIDRALHLKRPGGPPHVKLAIEWDSCVKESL--FGSLQEE RABIT19_11_PE2 NOISO LSLAC-SYLSPQDDRPLSQWAVDRALHLRKPGGPPHVKLAVEWDSSAKERL--FGSLQEE RAT10_PE1005 ENSRNOG00000003785 LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERL--FGSLQEE RAT10_PE1006 ISOFORMIN LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERL--FGSLQEE RAT10_PE1007 ISOFORMIN LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERL--FGSLQEE RAT10_PE1008 ISOFORMIN LSLAC-SYLSPQDSRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERL--FGSLQEE SORARGS_4441_PE1 NOISO LSVPC-SYLSPQDKRPLYHWAVDRVLNLRRPGGPPHIKLAVEWDSWAKECL--FGNLQEV TARSYGS_5169_PE1 NOISO XXXXX-XXXXXXXXXXXXXXXXXXALHLRKPGGPPHIKLAVEWDSSIKERL--FGSLQEE TETNGUNRD_PE1034 NOISO MALSM-LYLSEHFKNPLMSWTVFRALKLCGSGGPPHAKIVIEWENKIKECL--FGSIQEE TETNGUNRD_PE266 NOISO ILKQLPEHLSSC-ALFSLSTLLFRALKLCGSGGSPHVKLIIEWEHRMKDWSALFSS---- TURTRGS_195_1_PE4 NOISO LSLAC-SYLSPKDSQPLYHWTVDRALHLRRPGGPPHIKLAVEWDSGTKEHL--FGSIQEE XENTR255_2_PE8 NOISO GPASG-SYLSPKDPRPLQHPAIERALQLSGAGGPPHVKLAIEWEPKTKERL--FGNIQEE MATCH CONSENSUS ?S????SYLSP?D??PL?H??VDRAL?L??PGGPPH?KL??EW????KE?L??FG??QEE

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 RVQDADSVWRQQQAP-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCEALQRGMV-KLSL AILMEGL192808_1_1_PE2 ISOFORMIN RVQDADSVWRQQQAP-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCEALQRGMV-KLSL ANOCA79_5_PE1 NOISO VVEDAESVRMKQQLHLQQHSCTLDECFQLYTKEEQLAPDDAWRCPHCQVLQQGMV-KLSL BOVIN19_30_PE7 ENSBTAG00000000120 RVQDADSVRRQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWRCPHCQALQQGVV-KLSL BOVIN19_30_PE8 ISOFORMIN RVQDADSVRRQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWRCPHCQALQQGVV-KLSL BOVIN19_30_PE9 ISOFORMIN RVQDADSVRRQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWRCPHCQALQQGVV-KLSL CALJA5_74_PE21 ISOFORMIN RAQDADSVWQQQQAH-RQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMV-KLSL CALJA5_74_PE22 ENSCJAG00000012679 RAQDADSVWQQQQAH-RQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMV-KLSL CANFA5_38_PE5 NOISO RVQDADSVWRQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCEALQRGMV-KLSL CHOHOGS_5925_PE2 NOISO QVQEADSVWRQQQAH-QQPSCTLDECFQFYTKEGQ------------------------- CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 QVEVQDSVRLQKVLHQQPVTCTLEECFDLHTQVEELGADNAWLCPHCKKQQQGTVKKLSL DANRE3_27_PE27 NOISO VVKDSESVRAQQQHHVQQHSCTLDECFELYTKEEQLAPDDAWKCPHCKQLQQGTV-QMSL DANRE6_20_PE8 ENSDARG00000060632 VIEDAESVRIQQQNHVQQHSCTLDDCFQLYTKEEQLAPDDAWKCPHCKQMQQGMV-KMSL DANRE6_20_PE9 ISOFORMIN VIEDAESVRIQQQNHVQQHSCTLDDCFQLYTKEEQLAPDDAWKCPHCKQMQQGMV-KMSL DASNOGS_5350_PE2 NOISO RVEEAESVWRQRQAH-QQPSCTLDECFQFYTKEEQLA-DDAWKCPHCHVLQQGVV-KLSL DIPORGS_4986_PE2 NOISO RVQDADSVWRQQAAH-QQPSCTLDECFQSYTKEEQLAQDDAWKCPHCKVLQQGMV-KLSL ERIEUGS_5458_PE1 NOISO RVLDADSMSQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCKPLQQGVV-KSSL FELCAGS_3581_PE2 NOISO XXXXXXXXXXXXXXX-XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX-XXXX FUGRU26_1_PE135 NOISO VVKDAESVRNQQQQHLQQHSCTLDECFQLYTKEEQLAPDDAWKCPHCKQLQQGMV-KMSL FUGRU65_1_PE42 NOISO VVKDAENVRNQHQ-YIHQYSCTLDECFYLYTKEEQLAPDDAWKCPHCKQLEQGMV-KMSL GASACGROUPIX_18_PE15 NOISO VIKDAESVRNQQQQHLQQHSCTLDECFQLYTKEEQLAPDDAWRCPHCKQLQQGMV-KMSL GASACGROUPXI_4_PE54 ISOFORMIN VVKDADSVRNQQQHHVQQYSCTLDECFQLYTKEEQLAPDDAWKCPHCKQLQQGMV-KMSL GASACGROUPXI_4_PE55 ENSGACG00000006879 VVKDADSVRNQQQHHVQQYSCTLDECFQLYTKEEQLAPDDAWKCPHCKQLQQGMV-KMSL GORGO17_11_PE4 NOISO RAQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMV-KLSL HORSE11_55_PE3 NOISO RVQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWRCPHCKALQQGMV-KLSL HS17_PE534 ENSG00000154914 RAQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMV-KLSL LOXAF47_14_PE7 ENSLAFG00000014238 RVQDAESVWQQQQAH-QQPSCTLDECFQFYTKEEQLTQDDAWKCPHCKVLQQGTV-KLSL LOXAF47_14_PE8 ISOFORMIN RVQDAESVWQQQQAH-QQPSCTLDECFQFYTKEEQLTQDDAWKCPHCKVLQQGTV-KLSL MACEUGS_6207_PE1 NOISO VVQDAPSVQLQQQAH-QQPSCTLNECFQLYTKEEQXXXXXXXXXXXXXXXXXXXX-XXXX MACMU16_10_PE4 ENSMMUG00000014039 RAQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMV-KLSL MICMUGS_287_PE4 NOISO RARDADSVWRQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCKVLQQGMV-KLSL MONDO2_262_PE1 NOISO VVQDAPSVQLQQQAH-LQPSCTLNECFQLYTKEEQLAPDDAWKCPHCKILQQGMV-KLSL MOUSE11_PE1552 ENSMUSG00000020905 RVQDADSVWRQQQAH-QQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVV-KLSL MOUSE11_PE1553 ISOFORMIN RVQDADSVWRQQQAH-QQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVV-KLSL MOUSE11_PE1554 ISOFORMIN RVQDADSVWRQQQAH-QQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVV-KLSL OCHPRGS_5271_PE1 NOISO RVQDADSVWRQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVPQQGTV-KLSL ORYLA1_12_PE4 NOISO VVQDAESVRNQQQQHLQQHSCTLDECFQLYTKEEQLAPDDAWKCPHCKQLQQGMV-KMNL PANTR17_49_PE8 NOISO RAQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMV-KLSL PIG12_54_PE2 NOISO RVQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWRCPHCQALQQGVV-KLSL PONPY17_11_PE3 NOISO RAQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGMV-KLSL PROCAGS_5529_PE2 NOISO XXXXXXXXXXXXXXX-XXXXXXXXXXXXXXXXXXXLTQDDAWKCPHCKVLQQGTV-KLSL PTEVAGS_2827_PE2 NOISO RVQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCSHCKVLQQGMV-KLSL RABIT19_11_PE2 NOISO RVQDADSVRRQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCQVLQQGTV-KLSL RAT10_PE1005 ENSRNOG00000003785 RVQDADSVWRQQQAH-QQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVV-KLSL RAT10_PE1006 ISOFORMIN RVQDADSVWRQQQAH-QQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVV-KLSL RAT10_PE1007 ISOFORMIN RVQDADSVWRQQQAH-QQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVV-KLSL RAT10_PE1008 ISOFORMIN RVQDADSVWRQQQAH-QQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVV-KLSL SORARGS_4441_PE1 NOISO QADS---V-QQQRAH-QQHSCTLDECFHLYTKEEQLAQDDAWKCPHCKVLQQGVM-KLSL TARSYGS_5169_PE1 NOISO RAQDADSVWQQQQAH-QQHSCTLDECFQFYTKEEQLAQDDAWKCPHCKVLQQGMV-KLSL TETNGUNRD_PE1034 NOISO VVKDAESVRNQQQQHLQQHSCTLDECFQLYTKEEQLAPDDAWKCPHCKQLQQGMV-KMSL TETNGUNRD_PE266 NOISO ----SVLHPRNQQQHIHQYSCTLDECFYLYTKEEQLAPDDAWKCPYCKQLQQGMV-KMSL TURTRGS_195_1_PE4 NOISO RVQDADSVWRQQQAH-QQQSCSLDECFQFYTKEEQLAQDDAWRCPHCQALQQGVV-KLSL XENTR255_2_PE8 NOISO VVHDDESVRAQQLEH-QQQSCTLDECFQLYTKEEQLAPDDAWRCPYCKVLQQGTV-KLSL MATCH CONSENSUS ???DA?SV??QQQ?H?QQ?SCTLDECFQ?YTKEEQLA?DDAW?CPHC??LQQG?V?K?SL

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 WTLPDILIVHLKRFCQLGERRHKLSTLVKFPLSGLNMAPHVAQRSAG--PPPAMGPRP-P AILMEGL192808_1_1_PE2 ISOFORMIN WTLPDILIVHLKRFCQLGERRHKLSTLVKFPLSGLNMAPHVAQRSAG--PPPAMGPRP-P ANOCA79_5_PE1 NOISO WTLPDILIIHLKRFRQVGEQRHKLSTLVRFPLYGLDMAPHVAKRSRG-------SKWG-P BOVIN19_30_PE7 ENSBTAG00000000120 WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLAGLNMAPHVAQRSTS--PKAMPGAWPPS BOVIN19_30_PE8 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLAGLNMAPHVAQRSTS--PKAMPGAWPPS BOVIN19_30_PE9 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLAGLNMAPHVAQRSTS--PKAMPGAWPPS CALJA5_74_PE21 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS--PDAGLDPWP-S CALJA5_74_PE22 ENSCJAG00000012679 WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS--PDAGLDPWP-S CANFA5_38_PE5 NOISO WTLPDILIIHLKRFCQVGEKRNKLSTLVRFPLSGLDMAPHVAQRGAG--PPAATGPWP-S CHOHOGS_5925_PE2 NOISO ------------------ERRNKLSTLVEFPLVGLDMAPHVAQRSTG--PQPAPGPWP-S CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 LTLPDILVLHLKRFRVTDGRRTKLCTRVEFPVTGLNMSKHIFSGEMNGHIS-PQSPKSYN DANRE3_27_PE27 NOISO WTLPDILILHLKRFRQVGERRNKLSTLVHFPLTGLDMGRHMVQRTSCA---R--AP--PG DANRE6_20_PE8 ENSDARG00000060632 WTLPDILILHLKRFRQVGKRRNKLSTLIRFPVSALDMTPHVVKRSQSM---KNLAPLPST DANRE6_20_PE9 ISOFORMIN WTLPDILILHLKRFRQVGKRRNKLSTLIRFPVSALDMTPHVVKRSQSM---KNLAPLPST DASNOGS_5350_PE2 NOISO WTLPDILLIHLKRFCQGGERRNKLSTLVRFPXXXXXXXXXXXXXXXXXXXXXXXXXXX-- DIPORGS_4986_PE2 NOISO WTLPDILIIHLKRFCQVGEKRNKLSTLVKFPLSGLDMAPHVAQRSTS--SKPVPGPWP-S ERIEUGS_5458_PE1 NOISO WTLADILIIHLKRVCQGGERRNKLSTLVKFPLVGLNMAPSVAQKNPN----PGASPLGPX FELCAGS_3581_PE2 NOISO XXXXXXXXXXXXXXXXXXXXGNKLSTLVKFPLSGLNMAPHVAQRNAG--PKPATGPWP-- FUGRU26_1_PE135 NOISO WTLPDILILHLKRFRQVGERRNKLSTPVRFPMAGLDMAPHVVKRSQSV-KNVNTGAWPPS FUGRU65_1_PE42 NOISO WTLPDILILHLKRFMQDGEHRTKLTTFVHFPLNGLDMAPHMVNHNHGV---H--PPPLTS GASACGROUPIX_18_PE15 NOISO WTLPDILILHLKRFRQVGERRNKLSTLVRFPLTGLDMAPHVVKRSQSM-RNLSSGAWPPP GASACGROUPXI_4_PE54 ISOFORMIN WTLPDILILHLKRFRQVGERRTKLTTFVHFPLAGLDMAPHMVSRGP-H---Q--PPLQPG GASACGROUPXI_4_PE55 ENSGACG00000006879 WTLPDILILHLKRFRQVGERRTKLTTFVHFPLAGLDMAPHMVSRGP-H---Q--PPLQPG GORGO17_11_PE4 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS--PEAGLGPWP-S HORSE11_55_PE3 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVEFPLSGLDMAPHVAQRSTS--PKPEPGPWP-S HS17_PE534 ENSG00000154914 WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS--PEAGLGPWP-S LOXAF47_14_PE7 ENSLAFG00000014238 WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAPRSTG--PQPGSGPWP-S LOXAF47_14_PE8 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAPRSTG--PQPGSGPWP-S MACEUGS_6207_PE1 NOISO XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX---XX-XXXX-X MACMU16_10_PE4 ENSMMUG00000014039 WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS--PEAGLGPWP-S MICMUGS_287_PE4 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS--PTPGPGPWP-P MONDO2_262_PE1 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLFGLNMAPHVAKRSLG--QEHLMNSWP-S MOUSE11_PE1552 ENSMUSG00000020905 WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTN--SKAGPGPWS-S MOUSE11_PE1553 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTN--SKAGPGPWS-S MOUSE11_PE1554 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTN--SKAGPGPWS-S OCHPRGS_5271_PE1 NOISO WTLPDILIIHLKRFCQVGEKRNKLSTLVKFPLSGLNMAPHMAQSSSVGPTPPGPGPWP-S ORYLA1_12_PE4 NOISO WTLPDILILHLKRFRQVGERRNKLSTLVGFPLTGLDMAPHVVKRSQSM-KNLHQGSSQTN PANTR17_49_PE8 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS--PEAGLGPWP-S PIG12_54_PE2 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLDMAPHVAQRSTG--PKGAPGPWP-S PONPY17_11_PE3 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS--PEAGLGPWP-S PROCAGS_5529_PE2 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLAGLNMAPHVAPRSIG--PQPSPGPWA-S PTEVAGS_2827_PE2 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLCGLNMAPHVAQRSAG--PKLEPGSWT-S RABIT19_11_PE2 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHMAPRGSS--ATTGPGPWP-S RAT10_PE1005 ENSRNOG00000003785 WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTN--SKAGPGPWS-S RAT10_PE1006 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTN--SKAGPGPWS-S RAT10_PE1007 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTN--SKAGPGPWS-S RAT10_PE1008 ISOFORMIN WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTN--SKAGPGPWS-S SORARGS_4441_PE1 NOISO WTLPDILIVHLKRFCQVRERRNKLSTLVKFPLSGLNMAPHVAKRSAS--ARSVRGPLP-S TARSYGS_5169_PE1 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLSGLNMAPHVAQRSTS----PKPGPWP-S TETNGUNRD_PE1034 NOISO WTLPDILILHLKRFRQVGERRNKLSDPVRFPMAGLDMAPHVVKRSQST-KNVNRGAWPQS TETNGUNRD_PE266 NOISO WTLPDILILHLKRFTHAGEHRNKLTTFVHFPLSGLDMAPHMVNHSHVA----HPPPL-TS TURTRGS_195_1_PE4 NOISO WTLPDILIIHLKRFCQVGERRNKLSTLVKFPLAGLDMAPHVAQRSTG--PKASPGPWPPS XENTR255_2_PE8 NOISO WTLPDILIIHLKRFRQVGGRRNKLSTLVRFPLAGLDMAPHVVKRSQG--GKGMLGQWA-S MATCH CONSENSUS WTLPDILI?HLKRF?QVGERRNKLSTLV?FPL?GL?MAPHV??R????????????????

AILMEGL192808_1_1_PE1 ENSAMEG00000001973 WKQPAGL-----PPGYPLDFL--YDLYAVCNHHGTLQGGHYTAYCRNSLDGQWYSYDDST AILMEGL192808_1_1_PE2 ISOFORMIN WKQPAGL-----PPGYPLDFL--YDLYAVCNHHGTLQGGHYTAYCRNSLDGQWYSYDDST ANOCA79_5_PE1 NOISO WKQPT-------PESNQCNFL--YDLYAVCNHHGSMQGGHYTAYCRNALDGHWYSYDDST BOVIN19_30_PE7 ENSBTAG00000000120 WKQPACL-----PTSYPPDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDSM BOVIN19_30_PE8 ISOFORMIN WKQPACL-----PTSYPPDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDSM BOVIN19_30_PE9 ISOFORMIN WKQPACL-----PTSYPPDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDSM CALJA5_74_PE21 ISOFORMIN WKRPGCL-----PTSYPLDFL--YDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDST CALJA5_74_PE22 ENSCJAG00000012679 WKRPGCL-----PTSYPLDFL--YDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDST CANFA5_38_PE5 NOISO WKQPACL-----PAGYPLDSL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST CHOHOGS_5925_PE2 NOISO WKQPACL-----PHSCPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST CIOSAREFTIG_1_6_PE37 ENSCSAVG00000011633 WNPAFA--SHLPEMVGPEDHL--YDLYAVCNHFGNLNSGHYTACCSNPLNGKW------- DANRE3_27_PE27 NOISO WNKQAHR--RPEASRYATDVL--YDLYAVCNHHGGMHGGHYTAYCRNSVDGQWYTYDDSS DANRE6_20_PE8 ENSDARG00000060632 WKH------------GETDFL--YDLYAVCNHHGGMHGGHYTAYCRNSVDGHWYSYDDSS DANRE6_20_PE9 ISOFORMIN WKH------------GETDFL--YDLYAVCNHHGGMHGGHYTAYCRNSVDGHWYSYDDSS DASNOGS_5350_PE2 NOISO ---XXXX-----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYCRNSLDGQWYSYDDST DIPORGS_4986_PE2 NOISO WQQPTHL-----PTAYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST ERIEUGS_5458_PE1 NOISO XXXXXXX-----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYSYDDST FELCAGS_3581_PE2 NOISO SEQPACL-----TASCPWDFL--YDLYAVCNHHGGLQGGHYTAYCRNSLDGQWYSFDDST FUGRU26_1_PE135 NOISO WKQSSGKQQQPADLILPRDYL--YDLYAVCNHHGGMHGGHYTAYCRNSVDGQWYSYDDSS FUGRU65_1_PE42 NOISO WKQCQ----RRE--LIPPEFL--YDLYAVCNHHGGMHGGHYTAFCRNSVDGQWYSYDDSS GASACGROUPIX_18_PE15 NOISO CKLPSGHHPQAASAVLPHDYL--YDLYAVCNHHGGMHGGHYTAYCRNSVDGQWYSYDDSS GASACGROUPXI_4_PE54 ISOFORMIN WTQPR----RPD--LAPPDFL--YDLYAVCNHHGGMHGGHYTAFCRNSVDGQWYGFDDSS GASACGROUPXI_4_PE55 ENSGACG00000006879 WTQPR----RPD--LAPPDFL--YDLYAVCNHHGGMHGGHYTAFCRNSVDGQWYGFDDSS GORGO17_11_PE4 NOISO WKQPDCL-----PTSYPLDFL--YDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDST HORSE11_55_PE3 NOISO WRQPACL-----PTSYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST HS17_PE534 ENSG00000154914 WKQPDCL-----PTSYPLDFL--YDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDST LOXAF47_14_PE7 ENSLAFG00000014238 WKQPTCL-----PNGYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGRWYSYDDST LOXAF47_14_PE8 ISOFORMIN WKQPTCL-----PNGYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGRWYSYDDST MACEUGS_6207_PE1 NOISO XXXXXXX-------XXXXXXX--XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX MACMU16_10_PE4 ENSMMUG00000014039 WKPPGCL-----PTSCPLDFL--YDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDST MICMUGS_287_PE4 NOISO WKEPGCL-----PTAYPPDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST MONDO2_262_PE1 NOISO WEQASCL-----PNNCSFDFL--YDLYAVCNHHGSMQGGHYTAYCRNSLDGQWYSYDDST MOUSE11_PE1552 ENSMUSG00000020905 WKQPICL-----PTTYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST MOUSE11_PE1553 ISOFORMIN WKQPICL-----PTTYPLDFL--YDLYAVCNHHGSLQ-----AYCRNSLDGQWYSYDDST MOUSE11_PE1554 ISOFORMIN WKQPICL-----PTTYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST OCHPRGS_5271_PE1 NOISO WKQPTCL-----PTTYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGRWYNYDDST ORYLA1_12_PE4 NOISO RRPSSG-QHQRSDIMLARDYL--YDLYAVCNHHGGMHGGHYTAYCRNSVDALWYSYDDSS PANTR17_49_PE8 NOISO WKQPDCL-----PTSYPLDFL--YDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDST PIG12_54_PE2 NOISO WKQPACL-----PTSYPPDFL--YDLYAVCNHQGSLQGGHYTAYCRNSLDGQWYSYDDST PONPY17_11_PE3 NOISO WKQPDCL-----PTSYPLDFL--YDLYAVCNHHGNLQGGHYTAYCRNSLDGQWYSYDDST PROCAGS_5529_PE2 NOISO WKQSACL-----PNGYPPDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST PTEVAGS_2827_PE2 NOISO WKQPACL-----PTSYPLDFQ--YDLYAVCNHHGSLQGGHYTXXXXXXXXXXXXXXXXXX RABIT19_11_PE2 NOISO WQQPACL-----PTTYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGHWYSYDDST RAT10_PE1005 ENSRNOG00000003785 WKQPICL-----PTTYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST RAT10_PE1006 ISOFORMIN WKQPICL-----PTTYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST RAT10_PE1007 ISOFORMIN WKQPICL-----PTTYPLDFL--YDLYAVCNHHGSLQ-----AYCRNSLDGQWYSYDDST RAT10_PE1008 ISOFORMIN WKQPICL-----PTTYPLDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDDST SORARGS_4441_PE1 NOISO WKQPACL-----PTGCPLDFL--YDLYAVCNHHGSLQSGHYTAYCRNSLDGQWYNYDDSI TARSYGS_5169_PE1 NOISO WKQPTCL-----PTACPLDFL--YDLYAVCNHHGSLQGGHYTXXXXXXXXXXXXXXXXXX TETNGUNRD_PE1034 NOISO WRQSSGKQPQPADWILPHDYL--YDLYAVCNHHGGMHGGHYTAYCRNSVDGQWYSYDDSS TETNGUNRD_PE266 NOISO WNQCPR------RELIPPDFL--YDLYAVCNHHGGMHGGHYTAFCRNSVDGQWYSYDDSS TURTRGS_195_1_PE4 NOISO WKQPACL-----PTSYPPDFL--YDLYAVCNHHGSLQGGHYTAYCRNSLDGQWYSYDD