This phylogenetic tree is based on the alignment indicated below. CLUSTAL W(1.60) multiple sequence alignment gshs2fl ------------------------------------------------------------ O22494.TOMATO MGSGCSSPSISLTTIATSHFQSQESLSNSLNFYSPTRFLEPHLLKSSKIFIPKSPLKCAK O23732.BRAJU MVGGCSS----LSYSSSSTFIATTTLSSSLKLNPQSFIFHLNLRK------------RPP GSHB_ARATH ------------------------------------------------------------ Q21549.CAEEL ------------------------------------------------------------ GSHB_RAT ------------------------------------------------------------ GSHB_MOUSE ------------------------------------------------------------ GSHB_HUMAN ------------------------------------------------------------ GSHB_XENLA ------------------------------------------------------------ GSHB_SCHPO ------------------------------------------------------------ GSHB_YEAST ------------------------------------------------------------ gshs2fl ---------MTLPSFNDYHQIDDKVLENIVYDALVFATLNGLLVGDKSVERSGRVPGVGL O22494.TOMATO VPEMQTQLEDSAKPIVDPHDIDSKLVQKLANDALVWCPLRGLLVGDRNSERSGTIPGVDM O23732.BRAJU LRCLSSLTMESPKPILDFEKFDDGFVQKLVYDALVWSSLHGLVVGDKTHQRSGTVPGVGM GSHB_ARATH --------MESQKPIFDLEKLDDEFVQKLVYDALVWSSLHGLVVGDKSYQKSGNVPGVGL Q21549.CAEEL ---MAQKDDRILLLNAPRLPLEDDKLNELTADLHDWAHANGLVMRLSTDKLSS----EVC GSHB_RAT -----------MATSWGSILQDEKQLEELAQQAIDRALAEGVLLRSAKNPSSS----DVV GSHB_MOUSE -----------MATSWGSILQDEKQLEELAKQAIDRALAEGVLLRSAQHPSSS----DVV GSHB_HUMAN -----------MATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSS----EVV GSHB_XENLA -----------MADLWDDIYNDTKLLEELAPIAIDAALLQGVLMRTKESPNSS----DVV GSHB_SCHPO ---------------MEIEKYTPEQIEELGKGARDFAFAHGVVFTELSVSKEGR---NIA GSHB_YEAST ---------------MAHYPPSKDQLNELIQEVNQWAITNGLSMYPPKFEENP----SNA . . . *. gshs2fl VHLPFSLLPPPLPETHWKQACDLSPLFNELVDRVSLDGK----FLQQSLSKTKKAD-EFT O22494.TOMATO VHAPVALIPMSFPESHWKQACEVAPIFNELVDRVSQDGE----FLQQSLSRTRKAD-PFT O23732.BRAJU MHAPIALLPTPFPESYWNQACEVAPIFNELVDRISLDGK----FIQDSLSRTRKAD-VFT GSHB_ARATH MHAPIALLPTAFPEAYWKQACNVTPLFNELIDRVSLDGK----FLQDSLSRTKKVD-VFT Q21549.CAEEL QTTPLTLLPSPFPKNVFEEAVHIQNLFASLYHFIAYEFD----FLIDIHKNVVKTD-DFT GSHB_RAT TYAPFTLFPSPVPSTLLEQAYAVQMDFNILVDAVSQNSA----FLEQTLSSTIKKD-EYT GSHB_MOUSE TYAPFTLFPSPVPSALLEQAYAVQMDFNILVDAVSQNPA----FLEQTLSSTIKKD-DYT GSHB_HUMAN SYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAA----FLEQTLSSTIKQD-DFT GSHB_XENLA SFAPFALLPSPVPKALFEQAKCVQEDFNTLVDRISQDTS----FLEQVLSSTIKVD-DFI GSHB_SCHPO TQIPITLFPSVIPHGAFVEAVSVQKAYNKLYAKIANDYE----FLRLHLQSITKYD-EFM GSHB_YEAST SVSPVTIYPTPIPRKCFDEAVQIQPVFNELYARITQDMAQPDSYLHKTTEALALSDSEFT * .. * * .* . . * .. .. * . gshs2fl SRLLDIHAKM------LEMNKKEEIRMGLFRSDYMLDEKTK----SLLQIEMNTISTSFS O22494.TOMATO SRLLEIHSKM------LEINKLEEIRLGLHRSDYMLDEQTK----LLLQIELNTISSSFP O23732.BRAJU SRLLEIHSKM------LESNKREEIRLGLHRSDYMLDEETK----SLLQIEMNTISCSFP GSHB_ARATH SRLLDIHSKM------LERNKKEDIRLGLHRFDYMLDEETN----SLLQIEMNTISCSFP Q21549.CAEEL RNMVEILKKV------KAQGLKQPVTLAIQRSDYMCHKDQYSAEYGLKQIEINNIASSMG GSHB_RAT ARLFDIYKQV------LKEGIAQTVFLGLNRSDYMFQCSADGSK-ALKQIEINTISASFG GSHB_MOUSE ARLFDIYKQV------LKEGIAQTVFLGLNRSDYMFQCGADGSK-ALKQIEINTISASFG GSHB_HUMAN ARLFDIHKQV------LKEGIAQTVFLGLNRSDYMFQRSADGSP-ALKQIEINTISASFG GSHB_XENLA RRLFAIHKQV------QQEDCTQEVFLGINRSDYMFDCRDDGTP-ALKQIEINTIAASFG GSHB_SCHPO NKLWNLYQKHREAVAHLKENQFQPLSLGVFRSDYMVHQDDS--FIGCKQVEFNTISVSFG GSHB_YEAST GKLWSLYLAT----LKSAQYKKQNFRLGIFRSDYLIDKKKG--TEQIKQVEFNTVSVSFA . . . . . * **. *.* *... * gshs1 VTQLHRYILTCHGKLLGL---DSEKVPANNAVSQNAEALAKAWSEYN-------- gshs2fl GVGVVMTELHRNILSHYGKLLGL---DSERVPVNTADSRYAEALAKAWKEYN-------- O22494.TOMATO GLSCLVSELHRSLLQQYREDIAS---DPNRIPANNAVNQFAEALAKAWNEYG-------- O23732.BRAJU GFGRLVTELHQSLLRSHGDHLGL---DSKRVPRNGSTSQFADAMAKAWLEYN-------- GSHB_ARATH GLSRLVSQLHQSLLRSYGDQIGI---DSERVPINTSTIQFADALAKAWLEYS-------- Q21549.CAEEL AHALRLTEWHIRVLKALNISDDV---IQRAIPENKPIPMIAEALFKAWSHFS-------- GSHB_RAT GLASRTPAVHRHVLNVLNKTNE-----ASKILSNNPSKGLALGIAKAWELYG-------- GSHB_MOUSE GLASRTPAVHRHVLNVLNKTKE-----ASKILSNNPSKGLALGIAKAWELYG-------- GSHB_HUMAN GLASRTPAVHRHVLSVLSKTKE-----AGKILSNNPSKGLALGIAKAWELYG-------- GSHB_XENLA GLASRTPAVHQHVLKFLRKSEE-----SSSILTNDAVEGIGWGIAHAWALYG-------- GSHB_SCHPO GVSKAVSNLHAYCSQSGLYRKPL---TTNYLTVNTSVSGICTGISNAVDAYRDYVKNITS GSHB_YEAST GLSEKVDRLHSYLNRANKYDPKGPIYNDQNMVISDSGYLLSKALAKAVESYKS--QQSSS * . . * . gshs1 -------NPRAVIMTVVQAEERNMYDQHFLSAVLRDKHG--ITIVRKTMAEVDQEGEILP gshs2fl -------NPRAVIIVVVQGEERNMYDQHFVSAILREKHH--ITTIRKTLAEINQEGEILP O22494.TOMATO -------DPRAVIIFVVQAEERNMYDQHWLSASLRERHQ--VTTIRKTLAEIDALGELQQ O23732.BRAJU -------NPRAVVMVVVQPDERNMYDQHWLSSVLREKHN--IVTIRKSLAEVETEGRVHE GSHB_ARATH -------NPRAVVMVIVQPEERNMYDQHLLSSILREKHN--IVVIRKTLAEVEKEGSVQE Q21549.CAEEL -------NPAAVVLVVVENVNQNQIDQRHVEYELEKLGVPMTCIIRRNLTQCYEQLSLND GSHB_RAT -------SANAVVLLIAQEKERNIFDQRAIENELLDRK---IHVIRRRFEDVSERGSLDQ GSHB_MOUSE -------SANAVVLLIAQEKERNIFDQRAVENELLDRK---IHVIRGRFEDVSERGSLDQ GSHB_HUMAN -------SPNALVLLIAQEKERNIFDQRAIENELLARN---IHVIRRTFEDISEKGSLDQ GSHB_XENLA -------SVDATVMFLVENEQRNILDQRFIEAELCKRN---VRVIRRRLADVFERGTLDE GSHB_SCHPO KMNIASDNTKPIVLFVVKGGERNITDQRTLEYELLNRFH--VISKRIDIAELNSLIHDKS GSHB_YEAST ------TTSDPIVAFIVQRNERNVFDQKVLELNLLEKFG--TKSVRLTFDDVNDKLFIDD . . .* **. . * * gshs1 D-GTLSVNG----QAVAVIYFRAGYTPVDYPSESEWRARLLMEQSSAVKCPSISYHLVGT gshs2fl D-GTLSVDG----QAVAIVYFRAGYTPVDYPSESEWRARLLIEQSSAIKCPNISYHLVGT O22494.TOMATO D-GTLVVDG----QAVAVIYFRAGYAPSDYHSESEWKARLLMEQSRAVKCPSISYHLAGS O23732.BRAJU D-GTLTVGG----QAVSVVYYRSGYTPRDYPSESEWNARLLIEQSSAVKCPSIAYHLAGT GSHB_ARATH D-ETLIVGG----QAVAVVYFRSGYTPNDHPSESEWNARLLIEESSAVKCPSIAYHLTGS Q21549.CAEEL R-SDLMIDG----RQVAIVYFRAGYSPDHYPSTKEWEARERMELSTAIKTPWIGLQVANT GSHB_RAT N-RRLFMED----QEVAVVYFRDGYMPSQYNAQN-WEARLLLERSCAAKCPDIATQLAGT GSHB_MOUSE N-RRLFMDD----QEVAVVYFRDGYMPSQYNSQN-WEARLMLERSRAAKCPDIAIQLAGT GSHB_HUMAN D-RRLFVDG----QEIAVVYFRDGYMPRQYSLQN-WEARLLLERSHAAKCPDIATQLAGT GSHB_XENLA E-RHLFIDG----YEVAVAYFRTGYVPQDYTEQD-WEARLMLERSRAVKCPDVPTQLVGT GSHB_SCHPO S-NKLYMKTSFTTYEVAVVYYRVGYALDDYPSQEAWDMRLTIENTLAIKCPSISTHLAGS GSHB_YEAST KTGKLFIRDT--EQEIAVVYYRTGYTTTDYTSEKDWEARLFLEKSFAIKAPDLLTQLSGS * . ... *.* ** * * .* . * *.* . .. .. gshs1 KKIQQELAKPGVLERFLENK-DDIAKMRECFAGLWSLDDSD-----IVKKAIERPELFVM gshs2fl KKIQQELARPNVLERFFENK-EDIAKLRKCFAGLWSLEDSD-----IVNEAIAKPELFVM O22494.TOMATO KKIQQELAKPNVLERFLENK-DDIAKLRKCFAGLWSLDESD-----IVKDAIERPELYVM O23732.BRAJU KKIQQELAKPGVLERFMDNK-DDIAKLRKCFAGLWSLDDPE-----IIKKAIEKPELFVM GSHB_ARATH KKIQQELAKPGVLERFLDNK-EDIAKLRKCFAGLWSLDDSE-----IVKQAIEKPGLFVM Q21549.CAEEL KKTQQVLSEDGVLERFI-GKPREARDIRASFAGMWALENTDEVTMKVVAGAQKHPEAFVL GSHB_RAT KKVQQELSRVGLLEALLPGQPEAVARLRATFAGLYSLDMGEEG-DQAVAEALAAPSHFVL GSHB_MOUSE KKVQQELSRVGLLEALLPGQPEAVARLRATFAGLYSLDMGEEG-DQAIAEALAAPSHFVL GSHB_HUMAN KKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEG-DQAIAEALAAPSRFVL GSHB_XENLA KKVQQELSRPQILEKFLPDKPEAVARIRETFTGLYSLDIGEEG-DEAVRVALANPDQFVL GSHB_SCHPO KKIQQVLAESNALERFLEGD--ELQAVRSTFADMYPLDDTPRG-KEGIKLAFEKPEDFVL GSHB_YEAST KKIQQLLTDEGVLGKYISDA-EKKSSLLKTFVKIYPLDDTKLG-REGKRLALSEPSKYVL ** ** *. * . .* . *. * * .*. gshs1 KPQREGGGNNIYGDAVRDTLIKLQKTGSQEDAAYILMQRIFPNISAA-VLMRNGG-CHKD gshs2fl KPQREGGGNNIYGDDLRETLKRLQKPGSQEDAAYILMQRIFPANTAS-ILMRNGF-LHKD O22494.TOMATO KPQREGGGNNIYGEDVRGALLKLQKEGTGSDAAYILMQRIFPKISHS-ILMREGI-SHKE O23732.BRAJU KPQREGGGNNIYGDDVRENLLRLQREGEEENAAYILMQRIFPKVSNV-FLLRDGV-YHKD GSHB_ARATH KPQREGGGNNIYGDDVRENLLRLQKEGEEGNAAYILMQRIFPKVSNM-FLVREGV-YHKH Q21549.CAEEL KPQTEGGAALHTGDEMVQMLRELPE---EERGAFILMEKLKPMIIEN-YLVLAKKPITFA GSHB_RAT KPQREGGGNNFYGEEMVHALEQLKD--SEERASYILMEKIEPEPFRN-CLLRPGSPAQVV GSHB_MOUSE KPQREGGGNNLYGEEMVQALEQLKD--SEERASYILMEKIEPEPFRN-CLLRPGSPAQVV GSHB_HUMAN KPQREGGGNNLYGEEMVQALKQLKD--SEERASYILMEKIEPEPFEN-CLLRPGSPARVV GSHB_XENLA KPQREGGGNNLYGEELKEKLQECKD--SEERTSYILMDKINPKPLKN-CLLRAGGRVQIS GSHB_SCHPO KPQREGGGNNTYGKDIPGLLSKMPQ---EEWDSYILMRYINAVPSQN-YILKGER-PEKF GSHB_YEAST KPQREGGGNNVYKENIPNFLKGIEE---RHWDAYILMELIEPELNENNIILRDNK-SYNE *** *** . * ..*** . .. gshs1 HAISELGIFGTYLR--NKDRVVMNNQSGYLMRTKISSSDEGGVAAGFAVIDSVYLT--- gshs2fl HVISEFGIFSTYLR--DKDKVIVNNESGYMVRTKPSLFDEGGVLPGFGVIDSVYLT--- O22494.TOMATO ETISELGIYGTYLR--NKTEVLINQQAGYLMRTKVSSSDEGGVAAGFAVLDSIYLV--- O23732.BRAJU QAISELGVYGAYLR--NKERVIINEHSGYLMRTKVSSSDEGGVAAGYAVLDSIYLN--- GSHB_ARATH QAISELGVYGAYLR--SKDEVIVNEQSGYLMRTKIASSDEGGVAAGFGVLDSIYLI--- Q21549.CAEEL KAVSELGVYGYAFG--RKD-APELKTAGHLLRTKPESTAMGGVAAGHAVVDTPFLYEFI GSHB_RAT QCISELGIFGVYVR--QGTTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV--- GSHB_MOUSE QCISELGIFGVYVR--QGTTLVMNKHVGHLLRTKAVEHADGGVAAGVAVLDNPYPV--- GSHB_HUMAN QCISELGIFGVYVR--QEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV--- GSHB_XENLA ECISELGMFGVYVR--HRDQMIYYDQVGHLLRTKAIEHSDGGVAAGVAVLDNPYLV--- GSHB_SCHPO DVVDEIGILGTIVWNIKTDEVVQNGQSGFICRTKPKKTNEGGVATGYASLSSIELSE-- GSHB_YEAST PIISELGIYGCVLF--NDEQVLSNEFSGSLLRSKFNTSNEGGVAAGFGCLDSIILY--- . * *. * . *.* *** * .
Laurent Duret
Laboratoire de Biométrie, Génétique et Biologie des Populations.
UMR CNRS 5558
Université Claude Bernard - Lyon 1
43, bd. du 11 Novembre 1918
69622 Villeurbanne Cedex
France
Tel: +33 (0) 4 72 44 62 97
Fax: +33 (0) 4 78 89 27 19
E-mail:duret@biomserv.univ-lyon1.fr
January 7, 1999
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