Gn.Ex : gene number, exon number (for reference) Type : Init = Initial exon Intr = Internal exon Term = Terminal exon Sngl = Single-exon gene Prom = Promoter PlyA = poly-A signal S : DNA strand (+ = input strand; - = opposite strand) Begin : beginning of exon or signal (numbered on input strand) End : end point of exon or signal (numbered on input strand) Len : length of exon or signal (bp) Fr : reading frame (a codon ending at x is in frame f = x mod 3) Ph : net phase of exon (length mod 3) I/Ac : initiation signal or acceptor splice site score (x 10) Do/T : donor splice site or termination signal score (x 10) CodRg : coding region score (x 10) P : probability of exon (sum over all parses containing exon) Tscr : exon score (depends on length, I/Ac, Do/T and CodRg scores)
The PROBABILITY of a predicted exon is the estimated probability under GENSCAN's model of genomic sequence structure that the exon is correct. This probability depends in general on global as well as local sequence properties. This information can be used to assess the reliability of the predicted exon, e.g., it would be better to design PCR primers based on a predicted exon with probability > 0.95 than one with lower probability.