Under Construction

Detergent Structure in Membrane Protein Crystals

Analyse of two porin crystals
Penel S., Pebay-Peyroula E., Timmins P.,
Welte W., Hovers, J.,
 Rosenbusch J., Rummel G., Schirmer T.

  Despite the high importance of membrane proteins in biological function, the number of such structures which have been solved is very small, because of the difficulty in producing good quality crystals.  The detergent in which the protein has to be partially solubilised plays a crucial role particularly as it protects the hydrophobic transmembrane surface of the protein during the extraction of the protein from the membrane.

    A knowledge of the structure of detergents in protein crystals can yield a better understanding of the interactions between protein and membrane, the detergent playing the same role as the membrane, and of the effect of the detergent in the  crystallization process.  Using low resolution neutron crystallography  (DB21 diffractometer, Institut Laue-Langevin, Grenoble France) combined with  contrast variation [1], a well adapted technique for this study of disordered structures,  the structure of detergents in two membrane protein crystals, an E. coli porin (P321 crystal [2]) and a Rhodobacter capsulatus  porin (R3 crystal [3]), have been solved.

     In both studies, the detergent forms micellar structures around the transmembrane surface of the protein,  the micellar borders are limited by specific interactions with aromatic amino acids forming two rings playing a role in stabilizing the protein in the membrane.  Theses studies show that the  dimensions of the hydrophobic and  hydrophilic moieties of the detergent is a determinant factor in order to favour protein-protein contacts during the nucleation and the crystal growth.


Detergent Octyl-POE in P321 crystal
of porin OmpF of Rhodobacter  capsulatus [2]:
Detergent is pink, protein is yellow
View parallel to  c axis
View perpendicular to c axis
View perpendicular to c axis, aromatic amino acids are showed in green.
back to top of the page

Detergent C8E4  in R3 crystal
of porin form Escherichia coli.
Detergent is showed in white, protein in yellow
View parallel to  c axis
View perpendicular to c axis
View parallel to c axis, only the hydrophilic part of
the detergent (tails) are showed, in pink
back to top of the page

[1] Detergent structures studied by low resolution neutron crystallography