Annotation strategy and methods
Protein genes (CoDing Sequences):
- Search for start codons preceded by Ribosome Binding Sites (RBS) signals
- GeneMark method (periodic Markov chains)
- BLASTX and BLASTP sequence database scanning
- Emphasis on small genes and start codon assessment
- Development of specialized procedures for detecting putative sequencing errors
RNA genes:
- rRNA operons previously identified
- tRNAScan program for tRNA genes
Prediction of transcription terminators using Petrin
Integration of methods and results using Imagene