Detecting sequencing errors:BLASTX strategy

.

5'

3'

nucleic sequence

TRANSLATION

amino acid sequences

protein databank

+

{

SEARCHING FOR SIMILARITIES

“hits”

3'

+1

+2

+3

1

2

3

'Sequential' frameshift

'Overlapping' frameshift

from1

to1

to2

to3

to1 < from2

to2 > from3

5'

from2

from3

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