| Update* | Download complete package | |
| GEMINI v1.3.0 |
Download (.exe) 1,59 Mo | Download (.zip) 2,69 Mo |
*just replace the new .exe file in the GEMINI folder on you disk!
Download Gemini Software Guide (pdf, 235 Ko)
Download paper about Gemini published in Molecular Ecology notes (pdf, 284 Ko)
| Versions | What's new? |
|
v1.2.0 -> v1.3.0 July 2003 |
New approch is available for the construction of the consensus (probability method). Taberlet et al. 1996 method for multiple genotyping is now avalaible in simulations. Script.R file for analysing data for population size estimation has been modified. You can set a number of iterations for the estimation of population size via rarefaction curve method. |
| v1.1.0 -> v1.2.0 |
Changment in organisation of the menus An option is added in the Batch menu (Test Consensus Threshold) bugs fixed Help file updated |
| v1.0.0 -> v1.1.0 |
bugs fixed Help file updated |
Improvements in the determination of individual genotypes from samples with low DNA quantity and quality are of prime importance in molecular ecology and conservation for reliable genetic individual identification (molecular tagging using microsatllites loci). Thus, errors (e.g. allelic dropout and false allele) appearing during samples genotyping must be monitored and eliminated as far as possible. The multitubes approach, consisting on the replication of the PCR amplification per locus for confirming the genotype of a sample, is a very effective but money and time consuming solution.
GEMINI (Genotyping Error and Multitube approach for the INdividual Identification) is a program dedicated to researchers who want to test their future genetic analyses. GEMINI allows computing simulations to test and evaluate the mutlitubes approach and optimising the number of genotyping repetitions (or other parameters) by varying several parameters such as locus polymorphism, sampling design, rates of errors.
GEMINI conduct simulation of virtual population defined by the number of diploid individuals and their multilocus genotypes (at several microsatellites loci), simulation of he sampling and the genotyping (determination of the genotypes of each sample introducing errors). The identification of individuals is computed from the multilocus genotypes of samples (true genotypes) and genotyping results (with errors) to assess the impact of the genotyping errors on the genetic individual identification. To assess the reduction of the impact of the error using multiple tubes approach, GEMINI simulates a multitube protocol, in which, for each sample and locus, several repetitions are done to construct a consensus genotype for each sample and locus.
GEMINI is a Windows based software compiled with Visual Basic. You can install GEMINI on any Windows® compatible operating system. The installation of GEMINI required about 5 Mo on hard disk (about 2 Mo in the folder GEMINI and 3 Mo for system files). For simulations, CPU and memory must be sufficient (no test conducted). Files produced during the simulations could required large disk space.
All input and output files are text files (ASCII, space and tabulation separator), which could be written and read with any text editor (such as Word, WordPad, NoteBook) or Excel. GEMINI accepts all file extension. The user could choose all output names but, for convenience, the extension of the output file will be automatically ".txt".
Questions, comments and bugs reports should be directed to valiere@biomserv.univ-lyon1.fr, indicating in the subject of your email "GEMINI comments". All messages and responses will be redirect to any registered persons.
Package Setup.zip is composed by Setup.exe file and all dependancy files needed for the installation of GEMINI, Help file and Data file (constituting a example of all the file used and output). For installing GEMINI on your computer, double-click on Setup.exe (open Setup.zip using WINZIP available at http://www.winzip.com/) and follow the instructions. Installation requires about 5Mo disk space (about 2 Mo in the folder GEMINI and 3 Mo for system files). After installation, go on your Staring Menu/ Programs to launch GEMINI (Gemini icon), or go in the Gemini folder and double click on the Gemini.exe to run GEMINI.
If you want to download only the update, get Gemini.exe and place this file in the GEMINI folder already installed on your computer. Use this file to launch GEMINI.
Software and home page by Nathaniel Valière
at the Laboratoire de Biométrie et de Biologie Evolutive
Updated January 26, 2003