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<body bgcolor="#CCCCCC" text="#CCCCCC" link="#0000FF" vlink="#6666FF"> <p align="center"><b><font face="Verdana, Arial, Helvetica, sans-serif" size="+2"><a name="top"></a><font size="+3"><i><font color="#0000FF">GEMINI Software Home Page</font></i></font></font></b></p> <p align="center"><b><font size="-3"><b><font face="Verdana, Arial, Helvetica, sans-serif" size="+2"><img src="Splash2.jpg" width="124" height="77" border="1" align="absmiddle"></font></b></font></b></p> <p align="center"> </p> <div align="right"> <p align="right"><font size="+2"><a href="ftp://pbil.univ-lyon1.fr/pub/logiciel/gemini/">Go to the ftp site !!!</a></font></p> <p align="right"> </p> </div> <p><b><a name="news"></a>New downloads!</b></p> <table width="100%" border="1" cellpadding="1"> <tr> <td width="25%"><font color="#FF0000"><blink>Lastest version</blink></font></td> <td width="19%">Update*</td> <td width="34%">Download complete package</td> </tr> <tr> <td width="25%"><b><font color="#FF0000">GEMINI v1.2.0 <img src="new_flash_anim.gif" width="32" height="20" align="texttop"></font></b></td> <td width="19%"><a href="ftp://pbil.univ-lyon1.fr/pub/logiciel/gemini/gemini.exe">Download</a> (.exe)</td> <td width="34%"><a href="ftp://pbil.univ-lyon1.fr/pub/logiciel/gemini/setup.zip">Download</a> (.zip)</td> </tr> </table> <p><font size="2" face="Arial, Helvetica, sans-serif"><i>*just replace the new .exe file in the GEMINI folder on you disk!</i></font></p> <p align="right"> </p> <p><b><a name="historic"></a>Update events</b></p> <table width="98%" border="1"> <tr> <td>Versions</td> <td>What new?</td> </tr> <tr> <td><i>v1.0.0 -> v1.1.0</i></td> <td> <p>bugs fixed</p> <p>Help file updated</p> </td> </tr> <tr> <td><i>v1.1.0 -> v1.2.0</i></td> <td> <p>Changment in organisation of the menus</p> <p>Adding of an option in the Batch menu (<i>Test Consensus Threshold</i>)</p> <p>bugs fixed</p> <p>Help file updated</p> </td> </tr> </table> <p align="right"><a href="#top">Top of the page</a></p> <p><b><a name="description"></a>What is GEMINI?</b></p> <p>Improvements in the determination of individual genotypes from samples with low DNA quantity and quality are of prime importance in molecular ecology and conservation for reliable genetic individual identification (molecular tagging using microsatllites loci). Thus, errors (e.g. allelic dropout and false allele) appearing during samples genotyping must be monitored and eliminated as far as possible. The multitubes approach is a very effective but money and time consuming solution. </p> <p><b><i><font color="#FF0000">GEMINI </font></i></b>(<font color="#FF0000"><b>G</b></font>enotyping <font color="#FF0000"><b>E</b></font>rror and <font color="#FF0000"><b>M</b></font>ultitube approach for the <font color="#FF0000"><b>IN</b></font>dividual <b><font color="#FF0000">I</font></b>dentification) is a program dedicated to researchers who want to test their future genetic analyses. <b><i><font color="#FF0000">GEMINI</font></i></b> allows computing simulations to test and evaluate the mutlitubes approach and optimising the number of genotyping repetitions (or other parameters) by varying several parameters such as locus polymorphism, sampling design, rates of errors.</p> <p><b><i><font color="#FF0000">GEMINI</font></i></b> conduct simulation of virtual population defined by the number of diploid individuals and their multilocus genotypes (at several microsatellites loci), simulation of he sampling and the genotyping (determination of the genotypes of each sample introducing errors). The identification of individuals is computed from the multilocus genotypes of samples (true genotypes) and genotyping results (with errors) to assess the impact of the genotyping errors on the genetic individual identification. To assess the reduction of the impact of the error using multiple tubes approach, <b><i><font color="#FF0000">GEMINI</font></i></b> simulates a multitube protocol, in which, for each sample and locus, several repetitions are done to construct a consensus genotype for each sample and locus. </p> <p align="right"><a href="#top">Top of the page</a></p> <p><b><a name="config"></a>Configuration</b></p> <p><b><i><font color="#FF0000">GEMINI</font></i></b> is a Windows based software compiled with Visual Basic. You can install <b><i><font color="#FF0000">GEMINI</font></i></b> on a PC using Windows 95 or later operating system, or on an computer with OS (Macintosh) or Linux operating system with emulation for Windows 98. As <b><i><font color="#FF0000">GEMINI</font></i></b> could implement simulations, computer must have enough power and memory. For saving memory and running time, we recommend to not use any programs when GEMINI Software runs. </p> <p>All input and output files are text files (ASCII, space or tabulation separator), which could be written and read with any text editor (such as Word, WordPad, NoteBook) or Excel. <b><i><font color="#FF0000">GEMINI</font></i></b> accepts all file extension. The user could choose all output names but the extension of the output file will be automatically ".txt" (then you read the output files using any text editor by just double-clicking on the file).</p> <p>Questions, comments and bugs reports should be directed to <a href="mailto:valiere@biomserv.univ-lyon1.fr">valiere@biomserv.univ-lyon1.fr</a>, indicating in the subject of your email "GEMINI comments" and will be redirect at any persons that has send his e-mail adress.</p> <p align="right"><a href="#top">Top of the page</a></p> <p><b><a name="install"></a>Installation</b></p> <p align="left">Package is composed by Setup.exe file and all dependancy files needed for the installation of <b><i><font color="#FF0000">GEMINI</font></i></b>, Help file and Data file (constituting a example of all the file used and output). For installing <b><i><font color="#FF0000">GEMINI</font></i></b> on your computer, double-click on Setup.exe (if you download Gemini.zip, open it using WINZIP available at <a href="http://www.winzip.com/">http://www.winzip.com/</a>) and follow the instructions. Installation requires about 7Mo space on your HDD. After installation, go on your Staring Menu/ Programs to launch <b><i><font color="#FF0000">GEMINI</font></i></b> (Gemini icon), or go in the Gemini folder and double click on the Gemini.exe to run <b><i><font color="#FF0000">GEMINI</font></i></b>. </p> <p align="right"><a href="#top">Top of the page</a></p> </body>