SequenceTools.cpp

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// SPDX-FileCopyrightText: The Bio++ Development Group
//
// SPDX-License-Identifier: CECILL-2.1
 
#include <Bpp/Numeric/Matrix/Matrix.h>
#include <Bpp/Numeric/VectorTools.h>
 
#include "Alphabet/AlphabetTools.h"
#include "SequenceTools.h"
#include "StringSequenceTools.h"
 
using namespace bpp;
 
// From the STL:
#include <ctype.h>
#include <cmath>
#include <list>
#include <iostream>
 
using namespace std;
 
shared_ptr<RNY> SequenceTools::RNY_(new RNY(AlphabetTools::DNA_ALPHABET));
NucleicAcidsReplication SequenceTools::DNARep_(AlphabetTools::DNA_ALPHABET, AlphabetTools::DNA_ALPHABET);
NucleicAcidsReplication SequenceTools::RNARep_(AlphabetTools::RNA_ALPHABET, AlphabetTools::RNA_ALPHABET);
NucleicAcidsReplication SequenceTools::transc_(AlphabetTools::DNA_ALPHABET, AlphabetTools::RNA_ALPHABET);
 
/******************************************************************************/
 
bool SequenceTools::areSequencesIdentical(const SequenceInterface& seq1, const SequenceInterface& seq2)
{
  // Site's size and content checking
  if (seq1.getAlphabet()->getAlphabetType() != seq2.getAlphabet()->getAlphabetType())
    return false;
  if (seq1.size() != seq2.size())
    return false;
  else
  {
    for (size_t i = 0; i < seq1.size(); i++)
    {
      if (seq1[i] != seq2[i])
        return false;
    }
    return true;
  }
}
 
/******************************************************************************/
 
void SequenceTools::complement(SequenceInterface& seq)
{
  // Alphabet type checking
  NucleicAcidsReplication* NAR;
  if (AlphabetTools::isDNAAlphabet(seq.alphabet()))
  {
    NAR = &DNARep_;
  }
  else if (AlphabetTools::isRNAAlphabet(seq.alphabet()))
  {
    NAR = &RNARep_;
  }
  else
  {
    throw AlphabetException("SequenceTools::complement: Sequence must be nucleic.", seq.getAlphabet());
  }
  for (size_t i = 0; i < seq.size(); ++i)
  {
    seq.setElement(i, NAR->translate(seq.getValue(i)));
  }
}
 
/******************************************************************************/
 
unique_ptr<Sequence> SequenceTools::getComplement(const SequenceInterface& sequence)
{
  // Alphabet type checking
  NucleicAcidsReplication* NAR;
  if (AlphabetTools::isDNAAlphabet(sequence.alphabet()))
  {
    NAR = &DNARep_;
  }
  else if (AlphabetTools::isRNAAlphabet(sequence.alphabet()))
  {
    NAR = &RNARep_;
  }
  else
  {
    throw AlphabetException ("SequenceTools::getComplement: Sequence must be nucleic.", sequence.getAlphabet());
  }
 
  return NAR->translate(sequence);
}
 
/******************************************************************************/
 
unique_ptr<Sequence> SequenceTools::transcript(const Sequence& sequence)
{
  // Alphabet type checking
  if (!AlphabetTools::isDNAAlphabet(sequence.alphabet()))
  {
    throw AlphabetException ("SequenceTools::transcript : Sequence must be DNA", sequence.getAlphabet());
  }
 
  return transc_.translate(sequence);
}
 
/******************************************************************************/
 
unique_ptr<Sequence> SequenceTools::reverseTranscript(const Sequence& sequence)
{
  // Alphabet type checking
  if (!AlphabetTools::isRNAAlphabet(sequence.alphabet()))
  {
    throw AlphabetException ("SequenceTools::reverseTranscript : Sequence must be RNA", sequence.getAlphabet());
  }
 
  return transc_.reverse(sequence);
}
 
/******************************************************************************/
 
void SequenceTools::invert(SequenceInterface& seq)
{
  size_t seq_size = seq.size(); // store seq size for efficiency
  int tmp_state = 0; // to store one state when swapping positions
  size_t j = seq_size; // symmetric position iterator from sequence end
  for (size_t i = 0; i < seq_size / 2; ++i)
  {
    j = seq_size - 1 - i;
    tmp_state = seq.getValue(i);
    seq.setElement(i, seq.getValue(j));
    seq.setElement(j, tmp_state);
  }
}
 
/******************************************************************************/
 
unique_ptr<SequenceInterface> SequenceTools::getInvert(const SequenceInterface& sequence)
{
  auto iSeq = unique_ptr<SequenceInterface>(sequence.clone());
  invert(*iSeq);
  return iSeq;
}
 
/******************************************************************************/
 
void SequenceTools::invertComplement(SequenceInterface& seq)
{
  // Alphabet type checking
  NucleicAcidsReplication* NAR;
  if (AlphabetTools::isDNAAlphabet(seq.alphabet()))
  {
    NAR = &DNARep_;
  }
  else if (AlphabetTools::isRNAAlphabet(seq.alphabet()))
  {
    NAR = &RNARep_;
  }
  else
  {
    throw AlphabetException("SequenceTools::invertComplement: Sequence must be nucleic.", seq.getAlphabet());
  }
  // for (size_t i = 0 ; i < seq.size() ; i++) {
  //  seq.setElement(i, NAR->translate(seq.getValue(i)));
  // }
  size_t seq_size = seq.size(); // store seq size for efficiency
  int tmp_state = 0; // to store one state when swapping positions
  size_t j = seq_size; // symmetric position iterator from sequence end
  for (size_t i = 0; i < seq_size / 2; ++i)
  {
    j = seq_size - 1 - i;
    tmp_state = seq.getValue(i);
    seq.setElement(i, NAR->translate(seq.getValue(j)));
    seq.setElement(j, NAR->translate(tmp_state));
  }
  if (seq_size % 2)   // treat the state in the middle of odd sequences
  {
    seq.setElement(seq_size / 2, NAR->translate(seq.getValue(seq_size / 2)));
  }
}
 
/******************************************************************************/
 
double SequenceTools::getPercentIdentity(
    const SequenceInterface& seq1,
    const SequenceInterface& seq2,
    bool ignoreGaps)
{
  if (seq1.alphabet().getAlphabetType() != seq2.alphabet().getAlphabetType())
    throw AlphabetMismatchException("SequenceTools::getPercentIdentity", seq1.getAlphabet(), seq2.getAlphabet());
  if (seq1.size() != seq2.size())
    throw SequenceNotAlignedException("SequenceTools::getPercentIdentity", &seq2);
  int gap = seq1.alphabet().getGapCharacterCode();
  size_t id = 0;
  size_t tot = 0;
  for (size_t i = 0; i < seq1.size(); ++i)
  {
    int x = seq1.getValue(i);
    int y = seq2.getValue(i);
    if (ignoreGaps)
    {
      if (x != gap && y != gap)
      {
        tot++;
        if (x == y)
          id++;
      }
    }
    else
    {
      tot++;
      if (x == y)
        id++;
    }
  }
  return static_cast<double>(id) / static_cast<double>(tot) * 100.;
}
 
/******************************************************************************/
 
size_t SequenceTools::getNumberOfSites(const SequenceInterface& seq)
{
  size_t count = 0;
  auto& alpha = seq.alphabet();
  for (size_t i = 0; i < seq.size(); ++i)
  {
    if (!alpha.isGap(seq[i]))
      count++;
  }
  return count;
}
 
/******************************************************************************/
 
size_t SequenceTools::getNumberOfCompleteSites(const SequenceInterface& seq)
{
  size_t count = 0;
  auto& alpha = seq.alphabet();
  for (size_t i = 0; i < seq.size(); ++i)
  {
    if (!alpha.isGap(seq[i]) && !alpha.isUnresolved(seq[i]))
      count++;
  }
  return count;
}
 
/******************************************************************************/
 
unique_ptr<SequenceInterface> SequenceTools::getSequenceWithCompleteSites(const SequenceInterface& seq)
{
  auto& alpha = seq.alphabet();
  vector<int> content;
  for (size_t i = 0; i < seq.size(); ++i)
  {
    if (!(alpha.isGap(seq[i]) || alpha.isUnresolved(seq[i])))
      content.push_back(seq[i]);
  }
  auto newSeq = unique_ptr<SequenceInterface>(seq.clone());
  newSeq->setContent(content);
  return newSeq;
}
 
/******************************************************************************/
 
size_t SequenceTools::getNumberOfUnresolvedSites(const SequenceInterface& seq)
{
  size_t count = 0;
  auto alphaPtr = seq.getAlphabet();
  for (size_t i = 0; i < seq.size(); ++i)
  {
    if (alphaPtr->isUnresolved(seq[i]))
      count++;
  }
  return count;
}
 
/******************************************************************************/
 
unique_ptr<SequenceInterface> SequenceTools::getSequenceWithoutGaps(const SequenceInterface& seq)
{
  auto alphaPtr = seq.getAlphabet();
  vector<int> content;
  for (size_t i = 0; i < seq.size(); ++i)
  {
    if (!alphaPtr->isGap(seq[i]))
      content.push_back(seq[i]);
  }
  auto newSeq = unique_ptr<SequenceInterface>(seq.clone());
  newSeq->setContent(content);
  return newSeq;
}
 
/******************************************************************************/
 
void SequenceTools::removeGaps(SequenceInterface& seq)
{
  auto alphaPtr = seq.getAlphabet();
  for (size_t i = seq.size(); i > 0; --i)
  {
    if (alphaPtr->isGap(seq[i - 1]))
      seq.deleteElement(i - 1);
  }
}
 
/******************************************************************************/
 
unique_ptr<SequenceInterface> SequenceTools::getSequenceWithoutStops(const SequenceInterface& seq, const GeneticCode& gCode)
{
  auto calpha = dynamic_pointer_cast<const CodonAlphabet>(seq.getAlphabet());
  if (!calpha)
    throw Exception("SequenceTools::getSequenceWithoutStops. Input sequence should have a codon alphabet.");
  vector<int> content;
  for (size_t i = 0; i < seq.size(); ++i)
  {
    if (!gCode.isStop(seq[i]))
      content.push_back(seq[i]);
  }
  auto newSeq = unique_ptr<SequenceInterface>(seq.clone());
  newSeq->setContent(content);
  return newSeq;
}
 
/******************************************************************************/
 
void SequenceTools::removeStops(SequenceInterface& seq, const GeneticCode& gCode)
{
  auto calpha = dynamic_pointer_cast<const CodonAlphabet>(seq.getAlphabet());
  if (!calpha)
    throw Exception("SequenceTools::removeStops. Input sequence should have a codon alphabet.");
  for (size_t i = seq.size(); i > 0; --i)
  {
    if (gCode.isStop(seq[i - 1]))
      seq.deleteElement(i - 1);
  }
}
 
/******************************************************************************/
 
void SequenceTools::replaceStopsWithGaps(SequenceInterface& seq, const GeneticCode& gCode)
{
  auto calpha = dynamic_pointer_cast<const CodonAlphabet>(seq.getAlphabet());
  if (!calpha)
    throw Exception("SequenceTools::replaceStopsWithGaps. Input sequence should have a codon alphabet.");
  int gap = calpha->getGapCharacterCode();
  for (size_t i = 0; i < seq.size(); ++i)
  {
    if (gCode.isStop(seq[i]))
      seq.setElement(i, gap);
  }
}
 
/******************************************************************************/
 
unique_ptr<BowkerTest> SequenceTools::bowkerTest(
    const SequenceInterface& seq1,
    const SequenceInterface& seq2)
{
  if (seq1.size() != seq2.size())
    throw SequenceNotAlignedException("SequenceTools::bowkerTest.", &seq2);
  size_t n = seq1.size();
  auto alphaPtr = seq1.getAlphabet();
  unsigned int r = alphaPtr->getSize();
 
  // Compute contingency table:
  RowMatrix<double> array(r, r);
  int x, y;
  for (size_t i = 0; i < n; ++i)
  {
    x = seq1[i];
    y = seq2[i];
    if (!alphaPtr->isGap(x) && !alphaPtr->isUnresolved(x)
        && !alphaPtr->isGap(y) && !alphaPtr->isUnresolved(y))
    {
      array(static_cast<size_t>(x), static_cast<size_t>(y))++;
    }
  }
 
  // Compute Bowker's statistic:
  double sb2 = 0, nij, nji;
  for (unsigned int i = 1; i < r; ++i)
  {
    for (unsigned int j = 0; j < i; ++j)
    {
      nij = array(i, j);
      nji = array(j, i);
      if (nij != 0 || nji != 0)
      {
        sb2 += pow(nij - nji, 2) / (nij + nji);
      }
      // Else: we should display a warning there.
    }
  }
 
  // Compute p-value:
  double pvalue = 1. - RandomTools::pChisq(sb2, (r - 1) * r / 2);
 
  // Return results:
  auto test = make_unique<BowkerTest>();
  test->setStatistic(sb2);
  test->setPValue(pvalue);
  return test;
}
 
/******************************************************************************/
 
void SequenceTools::getPutativeHaplotypes(
    const SequenceInterface& seq,
    std::vector<unique_ptr<SequenceInterface>>& hap,
    unsigned int level)
{
  vector< vector< int >> states(seq.size());
  list< unique_ptr<Sequence>> t_hap;
  auto alphaPtr = seq.getAlphabet();
  unsigned int hap_count = 1;
  // Vector of available states at each position
  for (size_t i = 0; i < seq.size(); ++i)
  {
    vector<int> st = alphaPtr->getAlias(seq[i]);
    if (!st.size())
    {
      st.push_back(alphaPtr->getGapCharacterCode());
    }
    if (st.size() <= level)
    {
      states[i] = st;
    }
    else
    {
      states[i] = vector<int>(1, seq[i]);
    }
  }
  // Combinatorial haplotypes building (the use of tree may be more accurate)
  t_hap.push_back(make_unique<Sequence>(seq.getName() + "_hap" + TextTools::toString(hap_count++), "", alphaPtr));
  for (size_t i = 0; i < states.size(); ++i)
  {
    for (auto it = t_hap.begin(); it != t_hap.end(); it++)
    {
      for (size_t j = 0; j < states[i].size(); ++j)
      {
        auto tmp_seq = unique_ptr<Sequence>((**it).clone());
        tmp_seq->setName(seq.getName() + "_hap");
        if (j < states[i].size() - 1)
        {
          tmp_seq->setName(tmp_seq->getName() + TextTools::toString(hap_count++));
          tmp_seq->addElement(states[i][j]);
          t_hap.insert(it, std::move(tmp_seq));
        }
        else
        {
          (**it).addElement(states[i][j]);
        }
      }
    }
  }
  for (auto it = t_hap.rbegin(); it != t_hap.rend(); it++)
  {
    hap.push_back(std::move(*it));
  }
}
 
/******************************************************************************/
 
unique_ptr<Sequence> SequenceTools::combineSequences(
    const SequenceInterface& s1,
    const SequenceInterface& s2)
{
  if (s1.alphabet().getAlphabetType() != s2.alphabet().getAlphabetType())
  {
    throw AlphabetMismatchException("SequenceTools::combineSequences(const Sequence& s1, const Sequence& s2): s1 and s2 don't have same Alphabet.", s1.getAlphabet(), s2.getAlphabet());
  }
  auto alphaPtr = s1.getAlphabet();
  vector<int> st;
  vector<int> seq;
  size_t length = NumTools::max(s1.size(), s2.size());
  for (size_t i = 0; i < length; ++i)
  {
    if (i < s1.size())
      st.push_back(s1.getValue(i));
    if (i < s2.size())
      st.push_back(s2.getValue(i));
    seq.push_back(alphaPtr->getGeneric(st));
    st.clear();
  }
  auto s = make_unique<Sequence>(s1.getName() + "+" + s2.getName(), seq, alphaPtr);
  return s;
}
 
/******************************************************************************/
 
unique_ptr<Sequence> SequenceTools::subtractHaplotype(
    const SequenceInterface& s,
    const SequenceInterface& h,
    string name,
    unsigned int level)
{
  auto alphaPtr = s.getAlphabet();
  if (name.size() == 0)
    name = s.getName() + "_haplotype";
  string seq;
  if (s.size() != h.size())
    throw SequenceNotAlignedException("SequenceTools::subtractHaplotype: haplotype must be aligned with the sequence.", &h);
  for (unsigned int i = 0; i < s.size(); ++i)
  {
    string c;
    vector<int> nucs = alphaPtr->getAlias(s.getValue(i));
    if (nucs.size() > 1)
    {
      std::ignore = remove(nucs.begin(), nucs.end(), h.getValue(i));
      nucs = vector<int>(nucs.begin(), nucs.end() - 1);
    }
    else
    {
      nucs = vector<int>(nucs.begin(), nucs.end());
    }
    c = alphaPtr->intToChar(alphaPtr->getGeneric(nucs));
    if (level <= nucs.size() && (alphaPtr->isUnresolved(s.getValue(i)) || alphaPtr->isUnresolved(h.getValue(i))))
    {
      c = alphaPtr->intToChar(alphaPtr->getUnknownCharacterCode());
    }
    seq += c;
  }
  auto hap = make_unique<Sequence>(name, seq, alphaPtr);
  return hap;
}
 
/******************************************************************************/
 
unique_ptr<Sequence> SequenceTools::RNYslice(const SequenceInterface& seq, int ph)
{
  // Alphabet type checking
  if (AlphabetTools::isDNAAlphabet(seq.alphabet()))
  {
    throw AlphabetException ("SequenceTools::transcript : Sequence must be DNA", seq.getAlphabet());
  }
 
  if ((ph < 1) || (ph > 3))
    throw Exception("Bad phase for RNYSlice: " + TextTools::toString(ph) + ". Should be between 1 and 3.");
 
  size_t s = seq.size();
  size_t n = (s - static_cast<size_t>(ph) + 3) / 3;
 
  vector<int> content(n);
 
  int tir = seq.getAlphabet()->getGapCharacterCode();
  size_t j;
 
  for (size_t i = 0; i < n; i++)
  {
    j = i * 3 + static_cast<size_t>(ph) - 1;
 
    if (j == 0)
      content[i] = RNY_->getRNY(tir, seq[0], seq[1], *seq.getAlphabet());
    else
    {
      if (j == s - 1)
        content[i] = RNY_->getRNY(seq[j - 1], seq[j], tir, *seq.getAlphabet());
      else
        content[i] = RNY_->getRNY(seq[j - 1], seq[j], seq[j + 1], *seq.getAlphabet());
    }
  }
 
  // New sequence creating, and sense reversing
  auto alphaPtr = dynamic_pointer_cast<const Alphabet>(RNY_);
  auto seqPtr = make_unique<Sequence>(seq.getName(), content, seq.getComments(), alphaPtr);
 
  // Send result
  return seqPtr;
}
 
unique_ptr<Sequence> SequenceTools::RNYslice(const SequenceInterface& seq)
{
  // Alphabet type checking
  if (AlphabetTools::isDNAAlphabet(seq.alphabet()))
  {
    throw AlphabetException ("SequenceTools::transcript : Sequence must be DNA", seq.getAlphabet());
  }
 
  size_t n = seq.size();
 
  vector<int> content(n);
 
  int tir = seq.getAlphabet()->getGapCharacterCode();
 
  if (seq.size() >= 2)
  {
    content[0] = RNY_->getRNY(tir, seq[0], seq[1], *seq.getAlphabet());
 
    for (unsigned int i = 1; i < n - 1; i++)
    {
      content[i] = RNY_->getRNY(seq[i - 1], seq[i], seq[i + 1],
            *seq.getAlphabet());
    }
 
    content[n - 1] = RNY_->getRNY(seq[n - 2], seq[n - 1], tir, *seq.getAlphabet());
  }
 
  // New sequence creating, and sense reversing
  auto alphaPtr = dynamic_pointer_cast<const Alphabet>(RNY_);
  auto seqPtr = make_unique<Sequence>(seq.getName(), content, seq.getComments(), alphaPtr);
 
  // Send result
  return seqPtr;
}
 
/******************************************************************************/
 
 
void SequenceTools::getCDS(
    SequenceInterface& sequence,
    const GeneticCode& gCode,
    bool checkInit,
    bool checkStop,
    bool includeInit,
    bool includeStop)
{
  auto alphabet = dynamic_pointer_cast<const CodonAlphabet>(sequence.getAlphabet());
  if (!alphabet)
    throw AlphabetException("SequenceTools::getCDS. Sequence is not a codon sequence.", sequence.getAlphabet());
  if (checkInit)
  {
    size_t i;
    for (i = 0; i < sequence.size() && !gCode.isStart(sequence[i]); ++i)
    {}
    for (size_t j = 0; includeInit ? j < i : j <= i; ++j)
    {
      sequence.deleteElement(j);
    }
  }
  if (checkStop)
  {
    size_t i;
    for (i = 0; i < sequence.size() && !gCode.isStop(sequence[i]); ++i)
    {}
    for (size_t j = includeStop ? i + 1 : i; j < sequence.size(); ++j)
    {
      sequence.deleteElement(j);
    }
  }
}
 
/******************************************************************************/
 
size_t SequenceTools::findFirstOf(
    const SequenceInterface& seq,
    const SequenceInterface& motif,
    bool strict)
{
  if (motif.size() > seq.size())
    return seq.size();
  for (size_t seqi = 0; seqi < seq.size() - motif.size() + 1; seqi++)
  {
    bool match = false;
    for (size_t moti = 0; moti < motif.size(); moti++)
    {
      if (strict)
      {
        match = seq.getValue(seqi + moti) == motif.getValue(moti);
      }
      else
      {
        match = AlphabetTools::match(seq.getAlphabet(), seq.getValue(seqi + moti), motif.getValue(moti));
      }
      if (!match)
      {
        break;
      }
    }
    if (match)
    {
      return seqi;
    }
  }
  return seq.size();
}
 
/******************************************************************************/
 
unique_ptr<Sequence> SequenceTools::getRandomSequence(shared_ptr<const Alphabet>& alphabet, size_t length)
{
  int s = static_cast<int>(alphabet->getSize());
  vector<int> content(length);
  for (size_t i = 0; i < length; ++i)
  {
    content[i] = RandomTools::giveIntRandomNumberBetweenZeroAndEntry(s);
  }
  return make_unique<Sequence>("random", content, alphabet);
}
 
/******************************************************************************/