Prunier was tested on a simulated dataset available here. Prunier was designed to deal with real datasets, and was tested on maximum-likelihood (ML) gene trees. Prunier was compared to two programs, EEEP [4] and RIATA-HGT [5] that deal with the same biological problem, i.e. the reconciliation of a rooted reference tree with unrooted gene trees that are not necessary fully resolved. See Prunier's article for further details.
Prunier drives the Treefinder program to do ML tests (Slow version), to build ML gene trees (if not provided by the user) and to estimate branch lengths of the reference tree given the gene alignment (if needed for computations). Please install the Treefinder program before running Prunier in any directory of your PATH environment variable so that Prunier can run it. Then download below the appropriate binary of Prunier, and make it executable.
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